TY - JOUR

T1 - Thecochaos is not a myth: study of the genus Thecochaos (Amoebozoa, Discosea) – a rediscovered group of lobose amoeba, with short SSU gene

AU - Мезенцев, Елисей Сергеевич

AU - Бондаренко, Наталья Ивановна

AU - Камышацкая, Оксана Геннадьевна

AU - Насонова, Елена Станиславовна

AU - Глотова, Анна Андреевна

AU - Лойко, Сергей

AU - Истигечев, Георгий

AU - КУЛЕМЗИНА, Анстасия

AU - Абакумов, Евгений Васильевич

AU - Райко, Михаил Петрович

AU - Лапидус, Алла Львовна

AU - Смирнов, Алексей Валерьевич

N1 - Publisher Copyright: © 2022, Gesellschaft für Biologische Systematik.

PY - 2022/8/17

Y1 - 2022/8/17

N2 - Two multinucleate amoebae species, nowadays belonging to the genus Thecochaos, were never seen alive since 1913. Stained preparations deposited with the British Museum of Natural History (London) by E. Penard remain the only material proof of the existence of these organisms. We recently found an amoeba, identified as Thecochaos fibrillosum, in the soil sample originating from Chernevaya taiga soil in South Siberia (Russia). In total, we have got 15 cells of this species; however, using this amount of cells, we performed light- and electron-microscopic study, made permanent stained preparations, obtained SSU rRNA gene sequence using single-cell DNA isolation technique, and performed NGS sequencing to obtain genomic data for multigene analysis. Its SSU gene is unusually short (1586 bp), has significant losses in many laps, and, to the moment, is the shortest one known among Amoebozoa. The SSU sequence of this species robustly groups within the family Thecamoebidae as a sister to Thecamoeba/Sappinia clade. Multigene analysis either supported this position of Thecochaos or suggested its grouping with Sappinia. This study demonstrates that modern single-cell techniques allow correct and complete study and description of rare and almost non-cultivable amoebae species.

AB - Two multinucleate amoebae species, nowadays belonging to the genus Thecochaos, were never seen alive since 1913. Stained preparations deposited with the British Museum of Natural History (London) by E. Penard remain the only material proof of the existence of these organisms. We recently found an amoeba, identified as Thecochaos fibrillosum, in the soil sample originating from Chernevaya taiga soil in South Siberia (Russia). In total, we have got 15 cells of this species; however, using this amount of cells, we performed light- and electron-microscopic study, made permanent stained preparations, obtained SSU rRNA gene sequence using single-cell DNA isolation technique, and performed NGS sequencing to obtain genomic data for multigene analysis. Its SSU gene is unusually short (1586 bp), has significant losses in many laps, and, to the moment, is the shortest one known among Amoebozoa. The SSU sequence of this species robustly groups within the family Thecamoebidae as a sister to Thecamoeba/Sappinia clade. Multigene analysis either supported this position of Thecochaos or suggested its grouping with Sappinia. This study demonstrates that modern single-cell techniques allow correct and complete study and description of rare and almost non-cultivable amoebae species.

KW - evolution

KW - molecular phylogeny

KW - morphological systematics

KW - phylogenetic position

KW - species diversity

KW - Species diversity

KW - Molecular phylogeny

KW - Taxonomy

KW - Evolution

KW - Morphological systematics

KW - Phylogenetic position

UR - https://www.mendeley.com/catalogue/9f3ba4b1-6c98-3dd9-a591-e635d9b9a5d4/

UR - http://www.scopus.com/inward/record.url?scp=85136165178&partnerID=8YFLogxK

U2 - 10.1007/s13127-022-00581-9

DO - 10.1007/s13127-022-00581-9

M3 - Article

JO - Organisms Diversity and Evolution

JF - Organisms Diversity and Evolution

SN - 1439-6092

ER -