TY - JOUR

T1 - The Wound-Healing Effect of a Novel Fibroblasts-Impregnated Hydroxyethylcellulose Gel in a Rat Full-Thickness Burn Model: A Preclinical Study

AU - Novosad, Y.A.

AU - Shabunin, A.S.

AU - Enukashvily, N.I.

AU - Supilnikova, O.V.

AU - Konkina, A.I.

AU - Semenova, N.Y.

AU - Yatsemirsky, G.S.

AU - Zinoviev, E.V.

AU - Rodionova, K.N.

AU - Kryshen, K.L.

AU - Borodina, A.Y.

AU - Makarov, A.Y.

AU - Fedyuk, A.M.

AU - Nilov, A.D.

AU - Chikulaeva, E.V.

AU - Konkova, L.S.

AU - Chustrak, I.S.

AU - Traxova, V.V.

AU - Safonov, P.A.

AU - Vissarionov, S.V.

AU - Prikhodko, E.M.

AU - Yurkevich, Y.V.

N1 - Export Date: 10 November 2024 Сведения о финансировании: Ministry of Education and Science of the Russian Federation, Minobrnauka, 075-15-2021-1063/10 Сведения о финансировании: Ministry of Education and Science of the Russian Federation, Minobrnauka Текст о финансировании 1: The research was partially supported by the Ministry of Science and Higher Education of the Russian Federation under the Agreement No. 075-15-2021-1063/10, which provided funding for the expansion of fibroblasts and the quality control of the resulting cells used in the study.

PY - 2024/9/28

Y1 - 2024/9/28

N2 - Background/Objectives: The objective of this study was to assess the efficacy of a cell-containing wound dressing based on fibroblasts in hydroxyethylcellulose (HEC) gel for the local treatment of deep partial-thickness and/or full-thickness skin burns in an animal model. Methods: The rats (male Wistar, n = 100) were subjected to a full-thickness thermal burn (16 cm2). Radical necrectomy was performed one day after the burn. Three days later, the rats were randomly assigned to one of four groups: group 1 (no treatment), group 2 (chloramphenicol and methyluracil ointment, a routine clinical treatment), group 3 (a gel without cells, mock treatment), and group 4 (a dermal fibroblast-impregnated HEC gel). The treatment lasted for five days. The wound-healing process was evaluated by planimetric, cytologic, histologic, and immunohistochemical methods. Results: The differences in the rate of wound healing and the characteristics of wound cytology were identified. In the group 4, a regenerative type of cytogram was revealed, characterized by a significantly increased number of fibroblastic cells in comparison to samples from non-treated and mock-treated animals. Biopsy samples of burn wounds from animals in the group 4l demonstrated the presence of mature granulation tissue and a large number of microvessels. The repair process was stimulated, as evidenced by the increased thickness of newly formed granulation tissue and epidermis in the wound zone, elevated cellularity, and enhanced re-epithelialization activity. The number of Ki-67-positive proliferating cells was significantly higher in group 4 than in the control groups). A small number of non-proliferating donor fibroblasts was observed in the wound area 3 days after the end of treatment. Conclusions: The cell product is an effective agent for promoting wound healing during the regenerative phase. The experiments demonstrated that a gel populated by dermal fibroblasts can stimulate reparative regeneration processes in deep partial- and full-thickness burn wounds. © 2024 by the authors.

AB - Background/Objectives: The objective of this study was to assess the efficacy of a cell-containing wound dressing based on fibroblasts in hydroxyethylcellulose (HEC) gel for the local treatment of deep partial-thickness and/or full-thickness skin burns in an animal model. Methods: The rats (male Wistar, n = 100) were subjected to a full-thickness thermal burn (16 cm2). Radical necrectomy was performed one day after the burn. Three days later, the rats were randomly assigned to one of four groups: group 1 (no treatment), group 2 (chloramphenicol and methyluracil ointment, a routine clinical treatment), group 3 (a gel without cells, mock treatment), and group 4 (a dermal fibroblast-impregnated HEC gel). The treatment lasted for five days. The wound-healing process was evaluated by planimetric, cytologic, histologic, and immunohistochemical methods. Results: The differences in the rate of wound healing and the characteristics of wound cytology were identified. In the group 4, a regenerative type of cytogram was revealed, characterized by a significantly increased number of fibroblastic cells in comparison to samples from non-treated and mock-treated animals. Biopsy samples of burn wounds from animals in the group 4l demonstrated the presence of mature granulation tissue and a large number of microvessels. The repair process was stimulated, as evidenced by the increased thickness of newly formed granulation tissue and epidermis in the wound zone, elevated cellularity, and enhanced re-epithelialization activity. The number of Ki-67-positive proliferating cells was significantly higher in group 4 than in the control groups). A small number of non-proliferating donor fibroblasts was observed in the wound area 3 days after the end of treatment. Conclusions: The cell product is an effective agent for promoting wound healing during the regenerative phase. The experiments demonstrated that a gel populated by dermal fibroblasts can stimulate reparative regeneration processes in deep partial- and full-thickness burn wounds. © 2024 by the authors.

KW - burn wound

KW - deep partial-thickness burn

KW - fibroblasts

KW - full-thickness burn

KW - hydrogels

KW - regeneration

KW - regenerative medicine

KW - wound dressing

UR - https://www.mendeley.com/catalogue/d55945ab-b956-3d29-a503-7d7d142f649f/

U2 - 10.3390/biomedicines12102215

DO - 10.3390/biomedicines12102215

M3 - статья

C2 - 39457528

VL - 12

JO - Biomedicines

JF - Biomedicines

SN - 2227-9059

IS - 10

M1 - 2215

ER -