Research output: Contribution to journal › Article › peer-review
The uteroplacental contact zone cytokine influence on NK cell cytotoxicity to trophoblasts. / Bazhenov, Dmitriy; Mikhailova, Valentina; Nikolaenkov, Igor; Markova, Kseniya; Salloum, Zeina; Kogan, Igor; Gzgzyan, Aleksandr; Selkov, Sergey; Sokolov, Dmitriy.
In: Gynecological Endocrinology, Vol. 36, No. S1, 2020, p. 1-6.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - The uteroplacental contact zone cytokine influence on NK cell cytotoxicity to trophoblasts
AU - Bazhenov, Dmitriy
AU - Mikhailova, Valentina
AU - Nikolaenkov, Igor
AU - Markova, Kseniya
AU - Salloum, Zeina
AU - Kogan, Igor
AU - Gzgzyan, Aleksandr
AU - Selkov, Sergey
AU - Sokolov, Dmitriy
N1 - Funding Information: The study was supported by RFBR under grant No. 20-015-00014 (estimation of cytotoxic activity by flow cytometry); the government program No. ????-?19-119021290116-1 (cell culture) and No. ????-?20-120041390033-4 (method approbation). The participation of D.O.B. was funded by a Presidential Scholarship SP-2836.2018.4. Publisher Copyright: © 2020 The Author(s). Published by Informa UK Limited, trading as Taylor & Francis Group on behalf of the UR MED GRUPP (LLC). Copyright: Copyright 2020 Elsevier B.V., All rights reserved.
PY - 2020
Y1 - 2020
N2 - Objective: The present study was to estimate the role of cytokines for trophoblast death in NK cells presence. Methods: This study involves assessment of NK-92 line NK cell cytotoxic activity against JEG-3 line cells, in presence of cytokines. We also assessed the effect of secretory placenta products on NK cell cytotoxic activity toward JEG-3 line cells. Results: Uteroplacental contact zone cytokines are able to enhance trophoblast mortality both by themselves in case of IL-1β, IL-6, IFNγ, IL-4, TGFβ, bFGF, and also through increasing the cytotoxic potential of NK cells in case of IL-1β, IFNγ, IL-8, TGFβ, and GM-CSF. PLGF decreases NK cell cytotoxicity for trophoblasts. Secretory products of first trimester placenta enhance NK cell cytotoxic potential for trophoblasts. Conclusions: Cytokines of the uteroplacental contact zone can appear a mechanism ensuring trophoblast mortality dynamics throughout pregnancy.
AB - Objective: The present study was to estimate the role of cytokines for trophoblast death in NK cells presence. Methods: This study involves assessment of NK-92 line NK cell cytotoxic activity against JEG-3 line cells, in presence of cytokines. We also assessed the effect of secretory placenta products on NK cell cytotoxic activity toward JEG-3 line cells. Results: Uteroplacental contact zone cytokines are able to enhance trophoblast mortality both by themselves in case of IL-1β, IL-6, IFNγ, IL-4, TGFβ, bFGF, and also through increasing the cytotoxic potential of NK cells in case of IL-1β, IFNγ, IL-8, TGFβ, and GM-CSF. PLGF decreases NK cell cytotoxicity for trophoblasts. Secretory products of first trimester placenta enhance NK cell cytotoxic potential for trophoblasts. Conclusions: Cytokines of the uteroplacental contact zone can appear a mechanism ensuring trophoblast mortality dynamics throughout pregnancy.
KW - cytokines
KW - cytotoxicity
KW - JEG-3
KW - K562
KW - natural killer cells
KW - placenta
KW - Trophoblast
UR - http://www.scopus.com/inward/record.url?scp=85097493910&partnerID=8YFLogxK
U2 - 10.1080/09513590.2020.1816715
DO - 10.1080/09513590.2020.1816715
M3 - Article
C2 - 33305669
AN - SCOPUS:85097493910
VL - 36
SP - 1
EP - 6
JO - Gynecological Endocrinology
JF - Gynecological Endocrinology
SN - 0951-3590
IS - S1
ER -
ID: 74990382