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The upregulation of Ulk1-dependent autophagy does not require the p53 activity in mouse embryonic stem cells. / Vorobev, Mikhail L.; Alhasan, Bashar; Suvorova, Irina I.

In: Biochemical and Biophysical Research Communications, Vol. 552, 07.05.2021, p. 78-83.

Research output: Contribution to journal › Article › peer-review

Harvard

Vorobev, ML, Alhasan, B & Suvorova, II 2021, 'The upregulation of Ulk1-dependent autophagy does not require the p53 activity in mouse embryonic stem cells', Biochemical and Biophysical Research Communications, vol. 552, pp. 78-83. https://doi.org/10.1016/j.bbrc.2021.03.034

APA

Vorobev, M. L., Alhasan, B., & Suvorova, I. I. (2021). The upregulation of Ulk1-dependent autophagy does not require the p53 activity in mouse embryonic stem cells. Biochemical and Biophysical Research Communications, 552, 78-83. https://doi.org/10.1016/j.bbrc.2021.03.034

Vancouver

Vorobev ML, Alhasan B, Suvorova II. The upregulation of Ulk1-dependent autophagy does not require the p53 activity in mouse embryonic stem cells. Biochemical and Biophysical Research Communications. 2021 May 7;552:78-83. https://doi.org/10.1016/j.bbrc.2021.03.034

Author

Vorobev, Mikhail L. ; Alhasan, Bashar ; Suvorova, Irina I. / The upregulation of Ulk1-dependent autophagy does not require the p53 activity in mouse embryonic stem cells. In: Biochemical and Biophysical Research Communications. 2021 ; Vol. 552. pp. 78-83.

BibTeX

@article{bd10b375c57843f48645cb2edc5cbabf,
title = "The upregulation of Ulk1-dependent autophagy does not require the p53 activity in mouse embryonic stem cells",
abstract = "Autophagy is known to play a critical role in the early stages of embryogenesis including the formation of blastocyst. The existence of p53 protein-deficient mice may identify that p53 is not indispensable for the activation of autophagy in pluripotent cells derived from the inner cell mass of the blastocyst. We utilized a p53-knockout (KO) mouse embryonic stem cell (mESC) line to investigate the contribution of p53 in autophagy. We showed that lack of p53 has no effect on cell pluripotency but significantly hinders the differentiation process induced by retinoic acid. Using MRT68921, we revealed that Ulk1-dependent autophagy is activated in response to serum deprivation despite the deletion of p53 in mESCs. However, under retinoic acid-induced differentiation, the accumulation of autophagosomes and lysosomes is impaired in p53 KO mESCs, indicating a critical role of p53 in the regulation of autophagy upon differentiation.",
keywords = "Autophagy, Differentiation, Embryonic stem cells, Pluripotency, Ulk1, p53, p53(-/-)",
author = "Vorobev, {Mikhail L.} and Bashar Alhasan and Suvorova, {Irina I.}",
note = "Publisher Copyright: {\textcopyright} 2021 Elsevier Inc.",
year = "2021",
month = may,
day = "7",
doi = "10.1016/j.bbrc.2021.03.034",
language = "English",
volume = "552",
pages = "78--83",
journal = "Biochemical and Biophysical Research Communications",
issn = "0006-291X",
publisher = "Elsevier",

}

RIS

TY - JOUR

T1 - The upregulation of Ulk1-dependent autophagy does not require the p53 activity in mouse embryonic stem cells

AU - Vorobev, Mikhail L.

AU - Alhasan, Bashar

AU - Suvorova, Irina I.

N1 - Publisher Copyright: © 2021 Elsevier Inc.

PY - 2021/5/7

Y1 - 2021/5/7

N2 - Autophagy is known to play a critical role in the early stages of embryogenesis including the formation of blastocyst. The existence of p53 protein-deficient mice may identify that p53 is not indispensable for the activation of autophagy in pluripotent cells derived from the inner cell mass of the blastocyst. We utilized a p53-knockout (KO) mouse embryonic stem cell (mESC) line to investigate the contribution of p53 in autophagy. We showed that lack of p53 has no effect on cell pluripotency but significantly hinders the differentiation process induced by retinoic acid. Using MRT68921, we revealed that Ulk1-dependent autophagy is activated in response to serum deprivation despite the deletion of p53 in mESCs. However, under retinoic acid-induced differentiation, the accumulation of autophagosomes and lysosomes is impaired in p53 KO mESCs, indicating a critical role of p53 in the regulation of autophagy upon differentiation.

AB - Autophagy is known to play a critical role in the early stages of embryogenesis including the formation of blastocyst. The existence of p53 protein-deficient mice may identify that p53 is not indispensable for the activation of autophagy in pluripotent cells derived from the inner cell mass of the blastocyst. We utilized a p53-knockout (KO) mouse embryonic stem cell (mESC) line to investigate the contribution of p53 in autophagy. We showed that lack of p53 has no effect on cell pluripotency but significantly hinders the differentiation process induced by retinoic acid. Using MRT68921, we revealed that Ulk1-dependent autophagy is activated in response to serum deprivation despite the deletion of p53 in mESCs. However, under retinoic acid-induced differentiation, the accumulation of autophagosomes and lysosomes is impaired in p53 KO mESCs, indicating a critical role of p53 in the regulation of autophagy upon differentiation.

KW - Autophagy

KW - Differentiation

KW - Embryonic stem cells

KW - Pluripotency

KW - Ulk1

KW - p53

KW - p53(-/-)

UR - https://www.sciencedirect.com/science/article/pii/S0006291X21004174?via%3Dihub

UR - http://www.scopus.com/inward/record.url?scp=85102557664&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/2cc8f25f-be18-394e-bc33-18e1954d8d55/

U2 - 10.1016/j.bbrc.2021.03.034

DO - 10.1016/j.bbrc.2021.03.034

M3 - Article

VL - 552

SP - 78

EP - 83

JO - Biochemical and Biophysical Research Communications

JF - Biochemical and Biophysical Research Communications

SN - 0006-291X

ER -

ID: 76690691