Research output: Contribution to journal › Article
The size of DNA molecules and chromatin organization in the macronucleus of the ciliate Didinium nasutum (ciliophora). / Popenko, V.I.; Potekhin, A.A.; Karajan, B.P.; Skarlato, S.O.; Leonova, O.G.
In: Journal of Eukaryotic Microbiology, Vol. 62, No. 2, 2015, p. 260-264.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - The size of DNA molecules and chromatin organization in the macronucleus of the ciliate Didinium nasutum (ciliophora)
AU - Popenko, V.I.
AU - Potekhin, A.A.
AU - Karajan, B.P.
AU - Skarlato, S.O.
AU - Leonova, O.G.
PY - 2015
Y1 - 2015
N2 - © 2014 The Author(s) Journal of Eukaryotic Microbiology © 2014 International Society of Protistologists.Pulsed-field gel electrophoresis (PFGE) was applied to analyze the molecular karyotype of the ciliate Didinium nasutum. The data obtained indicate that D. nasutum belongs to the ciliate species with subchromosomal macronuclear genome organization. No short "gene-sized" DNA molecules were detected. Macronuclear DNAs formed a continuous spectrum from 50 kbp to approximately 1,000 kbp in size with a peak plateau between 250 and 400 kbp. The macronuclear DNA molecules were packed into chromatin bodies of 80-265 nm in size. Comparison of the PFGE and electron microscopic data shows that most if not all chromatin bodies contain more than one DNA molecule.
AB - © 2014 The Author(s) Journal of Eukaryotic Microbiology © 2014 International Society of Protistologists.Pulsed-field gel electrophoresis (PFGE) was applied to analyze the molecular karyotype of the ciliate Didinium nasutum. The data obtained indicate that D. nasutum belongs to the ciliate species with subchromosomal macronuclear genome organization. No short "gene-sized" DNA molecules were detected. Macronuclear DNAs formed a continuous spectrum from 50 kbp to approximately 1,000 kbp in size with a peak plateau between 250 and 400 kbp. The macronuclear DNA molecules were packed into chromatin bodies of 80-265 nm in size. Comparison of the PFGE and electron microscopic data shows that most if not all chromatin bodies contain more than one DNA molecule.
U2 - 10.1111/jeu.12161
DO - 10.1111/jeu.12161
M3 - Article
VL - 62
SP - 260
EP - 264
JO - Journal of Eukaryotic Microbiology
JF - Journal of Eukaryotic Microbiology
SN - 1066-5234
IS - 2
ER -
ID: 4012688