Research output: Contribution to journal › Article › peer-review
The shifts in the structure of the prokaryotic community of mountain-grassland soil under the influence of artificial larch plantations. / Ivanova, Ekaterina; Abakumov, Evgeny; Vasilyeva, Nadezda; Zverev, Alexei; Vladimirov, Artem; Ksenofontova, Natalia; Andronov, Evgeny; Kostenko, Igor.
In: PLoS ONE, Vol. 17, No. 2, e0263135, 18.02.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The shifts in the structure of the prokaryotic community of mountain-grassland soil under the influence of artificial larch plantations
AU - Ivanova, Ekaterina
AU - Abakumov, Evgeny
AU - Vasilyeva, Nadezda
AU - Zverev, Alexei
AU - Vladimirov, Artem
AU - Ksenofontova, Natalia
AU - Andronov, Evgeny
AU - Kostenko, Igor
N1 - Publisher Copyright: © 2022 Ivanova et al. This is an open access article distributed under the terms of the Creative Commons Attribution License, which permits unrestricted use, distribution, and reproduction in any medium, provided the original author and source are credited.
PY - 2022/2/18
Y1 - 2022/2/18
N2 - Creation of artificial forest plantations on a global scale is one of the ways to mitigate the negative effects of climate change on ecosystems, at the same time providing soil protection from erosion, regulation of the hydrological regime and carbon sequestration in soils of different natural and climatic zones. However, the change of the dominant plant community cause significant ecosystem changes, reflecting at the structure and functioning of the soil microbial complex as well. The shifts in prokaryotic community of the meadow soil resulting from the conversion of the native meadow (further grassland) phytocenosis to the artificial forest plantations was investigated with the use of NGS sequencing technology and metabarcoding approach–amplicon sequencing of V4 region of 16 S rRNA (performed on Illumina Miseq platform). The identified shifts in taxonomic structure and diversity may be the result of changes in the physic-chemical conditions of soils and, on the other hand, may serve as indicators of such changes. Cultivation of larch led to an increase in the diversity of the prokaryotic community and its stratification by depth. The acidifying effect of larch manifested itself in an increase in the proportion and diversity of acidobacteria, in the abundance of oligotrophic microorganisms of phyla Chloroflexi, Firmicutes, and a simultaneous comparative decrease in the bacteria of Verrucomicrobia phylum, alphaproteobacteria of or. Rhizobiales and Burkholderiales. The absence of clearly expressed dominants in the prokaryotic community, as well as a significant increase in alpha-diversity indices, compared with the control plot of native mountain-meadow soil under grassland vegetation, suggests a transitional nature of the soil ecosystem of artificial forest plantations.
AB - Creation of artificial forest plantations on a global scale is one of the ways to mitigate the negative effects of climate change on ecosystems, at the same time providing soil protection from erosion, regulation of the hydrological regime and carbon sequestration in soils of different natural and climatic zones. However, the change of the dominant plant community cause significant ecosystem changes, reflecting at the structure and functioning of the soil microbial complex as well. The shifts in prokaryotic community of the meadow soil resulting from the conversion of the native meadow (further grassland) phytocenosis to the artificial forest plantations was investigated with the use of NGS sequencing technology and metabarcoding approach–amplicon sequencing of V4 region of 16 S rRNA (performed on Illumina Miseq platform). The identified shifts in taxonomic structure and diversity may be the result of changes in the physic-chemical conditions of soils and, on the other hand, may serve as indicators of such changes. Cultivation of larch led to an increase in the diversity of the prokaryotic community and its stratification by depth. The acidifying effect of larch manifested itself in an increase in the proportion and diversity of acidobacteria, in the abundance of oligotrophic microorganisms of phyla Chloroflexi, Firmicutes, and a simultaneous comparative decrease in the bacteria of Verrucomicrobia phylum, alphaproteobacteria of or. Rhizobiales and Burkholderiales. The absence of clearly expressed dominants in the prokaryotic community, as well as a significant increase in alpha-diversity indices, compared with the control plot of native mountain-meadow soil under grassland vegetation, suggests a transitional nature of the soil ecosystem of artificial forest plantations.
KW - Bacteria/classification
KW - Forests
KW - Grassland
KW - High-Throughput Nucleotide Sequencing/methods
KW - Larix/growth & development
KW - Phylogeny
KW - RNA, Ribosomal, 16S/genetics
KW - Soil/chemistry
KW - Soil Microbiology
UR - http://www.scopus.com/inward/record.url?scp=85124924517&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/c112a390-b2d6-33b3-9a7f-bea5b80c784a/
U2 - 10.1371/journal.pone.0263135
DO - 10.1371/journal.pone.0263135
M3 - Article
C2 - 35180237
AN - SCOPUS:85124924517
VL - 17
JO - PLoS ONE
JF - PLoS ONE
SN - 1932-6203
IS - 2
M1 - e0263135
ER -
ID: 93165810