Research output: Contribution to journal › Article › peer-review
The role of trophic conditions in the regulation of physiology and metabolism of Chlamydomonas reinhardtii during batch culturing. / Puzanskiy, Roman; Shavarda, Alexey; Romanyuk, Daria; Shishova, Maria.
In: Journal of Applied Phycology, Vol. 33, No. 5, 10.2021, p. 2897-2908.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The role of trophic conditions in the regulation of physiology and metabolism of Chlamydomonas reinhardtii during batch culturing
AU - Puzanskiy, Roman
AU - Shavarda, Alexey
AU - Romanyuk, Daria
AU - Shishova, Maria
N1 - Puzanskiy, R., Shavarda, A., Romanyuk, D. et al. The role of trophic conditions in the regulation of physiology and metabolism of Chlamydomonas reinhardtii during batch culturing. J Appl Phycol 33, 2897–2908 (2021). https://doi.org/10.1007/s10811-021-02510-3
PY - 2021/10
Y1 - 2021/10
N2 - Chlamydomonas and other microalgae are capable of assimilating exogenous organic compounds. Additional carbon and energy sources affect different aspects of cell physiology. Simultaneous comparison of an auto- and mixotrophic batch of Chlamydomonas cultures indicates that acetate strongly stimulates the growth rate as well as the activity of respiration and photosynthesis in the exponential growth phase. In the middle of exponential growth, mixotrophy was characterized by a decrease in chlorophyll content that was followed by its elevation in the stationary phase. Chlamydomonas metabolomic profiles detected with GS-MS differed significantly as a result of trophic conditions. The maximum distinction was also observed in the middle of the exponential phase. The main feature of autotrophic cells is a higher carbohydrate content. Under the presence of acetate, cells demonstrated an intensive accumulation of fatty acids. This phenomenon is possibly related to the stimulation of lipid synthesis in the presence of additional sources of acetyl groups and by a higher content of carboxylates that were the result of elevated respiration. In the late stationary phase, mixotrophic cells exhibited a metabolomic shift towards autotrophy, probably due to acetate exhaustion. The importance of autotrophy at later stages of batch culture development correlated with chlorophyll accumulation in the early stationary phase. Thus, complicated trophic-induced metabolic and physiological alterations were revealed in Chlamydomonas cells during growth.
AB - Chlamydomonas and other microalgae are capable of assimilating exogenous organic compounds. Additional carbon and energy sources affect different aspects of cell physiology. Simultaneous comparison of an auto- and mixotrophic batch of Chlamydomonas cultures indicates that acetate strongly stimulates the growth rate as well as the activity of respiration and photosynthesis in the exponential growth phase. In the middle of exponential growth, mixotrophy was characterized by a decrease in chlorophyll content that was followed by its elevation in the stationary phase. Chlamydomonas metabolomic profiles detected with GS-MS differed significantly as a result of trophic conditions. The maximum distinction was also observed in the middle of the exponential phase. The main feature of autotrophic cells is a higher carbohydrate content. Under the presence of acetate, cells demonstrated an intensive accumulation of fatty acids. This phenomenon is possibly related to the stimulation of lipid synthesis in the presence of additional sources of acetyl groups and by a higher content of carboxylates that were the result of elevated respiration. In the late stationary phase, mixotrophic cells exhibited a metabolomic shift towards autotrophy, probably due to acetate exhaustion. The importance of autotrophy at later stages of batch culture development correlated with chlorophyll accumulation in the early stationary phase. Thus, complicated trophic-induced metabolic and physiological alterations were revealed in Chlamydomonas cells during growth.
KW - Autotrophy
KW - Batch culture
KW - Chlamydomonas
KW - Metabolomics
KW - Mixotrophy
KW - CARBON METABOLISM
KW - IRON-DEFICIENCY
KW - CHLORELLA-VULGARIS
KW - CHLOROPHYCEAE
KW - MIXOTROPHIC GROWTH
KW - ORTHOGONAL PROJECTIONS
KW - ORGANIC-CARBON
KW - ACETATE
KW - EXPRESSION
KW - ELECTRON-TRANSPORT
UR - http://www.scopus.com/inward/record.url?scp=85110464411&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/2e3564d6-fae9-39b7-94c3-daa71a44e147/
U2 - 10.1007/s10811-021-02510-3
DO - 10.1007/s10811-021-02510-3
M3 - Article
AN - SCOPUS:85110464411
VL - 33
SP - 2897
EP - 2908
JO - Journal of Applied Phycology
JF - Journal of Applied Phycology
SN - 0921-8971
IS - 5
ER -
ID: 86198450