Research output: Contribution to journal › Article › peer-review
The role of LMNA mutations in myogenic differentiation of C2C12 and primary satellite cells. / Perepelina, K. I.; Smolina, N. A.; Zabirnik, A. S.; Dmitrieva, R. I.; Malashicheva, A. B.; Kostareva, A. A.
In: Cell and Tissue Biology, Vol. 11, No. 3, 2017, p. 213-219.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The role of LMNA mutations in myogenic differentiation of C2C12 and primary satellite cells
AU - Perepelina, K. I.
AU - Smolina, N. A.
AU - Zabirnik, A. S.
AU - Dmitrieva, R. I.
AU - Malashicheva, A. B.
AU - Kostareva, A. A.
N1 - Perepelina, K.I., Smolina, N.A., Zabirnik, A.S. et al. The role of LMNA mutations in myogenic differentiation of C2C12 and primary satellite cells. Cell Tiss. Biol. 11, 213–219 (2017). https://doi.org/10.1134/S1990519X17030087
PY - 2017
Y1 - 2017
N2 - Nuclear lamins form nuclear lamina located under the inner nuclear membrane. It was believed that the nuclear lamina plays predominantly a structural role. Recently, its involvement in regulatory processes have been described, e.g., chromatin organization and gene transcription. It is known that mutations in the LMNA gene lead to development of laminopathies, primarily affecting tissues of mesenchymal origin. Today, the mechanisms of the lamina regulation of cell differentiation are largely unknown. In the present work, we studied the effect of LMNA gene mutations on the process of muscle differentiation of primary satellite cells and in С2С12 cell line. The genome of satellite and С2С12 cells was modified by cell transduction via lentiviral constructs encoding LMNA G232E associated with the development of Emery–Dreyfus muscular dystrophy and LMNA R571S associated with the development of dilated cardiomyopathy. Cell morphology was assessed with immunofluorescence, and expression of myogenic genes was analyzed by qPCR. We showed that the analyzed mutations reduced the cell ability to differentiate (to fuse and to form myotubes). We proposed that these mutations enhanced expression of early and reduced expression of late markers of myogenesis. Thus, mutations in nuclear lamins can modify the process of muscle differentiation.
AB - Nuclear lamins form nuclear lamina located under the inner nuclear membrane. It was believed that the nuclear lamina plays predominantly a structural role. Recently, its involvement in regulatory processes have been described, e.g., chromatin organization and gene transcription. It is known that mutations in the LMNA gene lead to development of laminopathies, primarily affecting tissues of mesenchymal origin. Today, the mechanisms of the lamina regulation of cell differentiation are largely unknown. In the present work, we studied the effect of LMNA gene mutations on the process of muscle differentiation of primary satellite cells and in С2С12 cell line. The genome of satellite and С2С12 cells was modified by cell transduction via lentiviral constructs encoding LMNA G232E associated with the development of Emery–Dreyfus muscular dystrophy and LMNA R571S associated with the development of dilated cardiomyopathy. Cell morphology was assessed with immunofluorescence, and expression of myogenic genes was analyzed by qPCR. We showed that the analyzed mutations reduced the cell ability to differentiate (to fuse and to form myotubes). We proposed that these mutations enhanced expression of early and reduced expression of late markers of myogenesis. Thus, mutations in nuclear lamins can modify the process of muscle differentiation.
KW - laminopathies
KW - muscle differentiation
KW - nuclear lamins
KW - primary satellite cells
KW - С2С12 cell line
UR - http://www.scopus.com/inward/record.url?scp=85020471567&partnerID=8YFLogxK
U2 - 10.1134/S1990519X17030087
DO - 10.1134/S1990519X17030087
M3 - Article
AN - SCOPUS:85020471567
VL - 11
SP - 213
EP - 219
JO - Cell and Tissue Biology
JF - Cell and Tissue Biology
SN - 1990-519X
IS - 3
ER -
ID: 35806557