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The Melanin Biosynthesis Gene from the CA15-1 Strain of Alfalfa Nodule Bacteria : Molecular Analysis and Phylogeny. / Chizhevskaya, E. P.; Naidenova, E. A.; Onishchuk, O. P.; Andronov, E. E.; Simarov, B. V.

In: Russian Journal of Genetics, Vol. 54, No. 8, 01.08.2018, p. 925-932.

Research output: Contribution to journal › Article › peer-review

Harvard

Chizhevskaya, EP, Naidenova, EA, Onishchuk, OP, Andronov, EE & Simarov, BV 2018, 'The Melanin Biosynthesis Gene from the CA15-1 Strain of Alfalfa Nodule Bacteria: Molecular Analysis and Phylogeny', Russian Journal of Genetics, vol. 54, no. 8, pp. 925-932. https://doi.org/10.1134/S1022795418080045

APA

Chizhevskaya, E. P., Naidenova, E. A., Onishchuk, O. P., Andronov, E. E., & Simarov, B. V. (2018). The Melanin Biosynthesis Gene from the CA15-1 Strain of Alfalfa Nodule Bacteria: Molecular Analysis and Phylogeny. Russian Journal of Genetics, 54(8), 925-932. https://doi.org/10.1134/S1022795418080045

Vancouver

Chizhevskaya EP, Naidenova EA, Onishchuk OP, Andronov EE, Simarov BV. The Melanin Biosynthesis Gene from the CA15-1 Strain of Alfalfa Nodule Bacteria: Molecular Analysis and Phylogeny. Russian Journal of Genetics. 2018 Aug 1;54(8):925-932. https://doi.org/10.1134/S1022795418080045

Author

Chizhevskaya, E. P. ; Naidenova, E. A. ; Onishchuk, O. P. ; Andronov, E. E. ; Simarov, B. V. / The Melanin Biosynthesis Gene from the CA15-1 Strain of Alfalfa Nodule Bacteria : Molecular Analysis and Phylogeny. In: Russian Journal of Genetics. 2018 ; Vol. 54, No. 8. pp. 925-932.

BibTeX

@article{cb1496dc847c4b93b528ebf3942b742f,
title = "The Melanin Biosynthesis Gene from the CA15-1 Strain of Alfalfa Nodule Bacteria: Molecular Analysis and Phylogeny",
abstract = "Identification and analysis of the melanin biosynthesis gene in the Sinorhizobium meliloti CA15-1 strain were carried out. Tn5 mutants, which lost the ability to synthesize melanin, were obtained. Molecular biological analysis of the mepA gene encoding tyrosinase was performed. In contrast to other members of the Rhizobiacea group, the identical structure of the mepA locus was revealed for all strains of nodule bacteria of the Sinorhizobium genus. Phylogenetic analysis indicated that horizontal transfer of the mepA gene occurred in the course of evolution of bacterial tyrosinases. At the same time, the closely related members of nodule bacteria “acquired” this gene from different sources. Analysis of symbiotic properties of the Mep– mutants of the CA15-1 strain showed that melanin did not affect the ability to go into an efficient symbiosis with alfalfa. Most probably, it is important only at the stages of adaptation of the free-living cells in the environment.",
keywords = "horizontal gene transfer, legume-rhizobium symbiosis, melanin biosynthesis, nodule bacteria, tyrosinase",
author = "Chizhevskaya, {E. P.} and Naidenova, {E. A.} and Onishchuk, {O. P.} and Andronov, {E. E.} and Simarov, {B. V.}",
year = "2018",
month = aug,
day = "1",
doi = "10.1134/S1022795418080045",
language = "English",
volume = "54",
pages = "925--932",
journal = "Russian Journal of Genetics",
issn = "1022-7954",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "8",

}

RIS

TY - JOUR

T1 - The Melanin Biosynthesis Gene from the CA15-1 Strain of Alfalfa Nodule Bacteria

T2 - Molecular Analysis and Phylogeny

AU - Chizhevskaya, E. P.

AU - Naidenova, E. A.

AU - Onishchuk, O. P.

AU - Andronov, E. E.

AU - Simarov, B. V.

PY - 2018/8/1

Y1 - 2018/8/1

N2 - Identification and analysis of the melanin biosynthesis gene in the Sinorhizobium meliloti CA15-1 strain were carried out. Tn5 mutants, which lost the ability to synthesize melanin, were obtained. Molecular biological analysis of the mepA gene encoding tyrosinase was performed. In contrast to other members of the Rhizobiacea group, the identical structure of the mepA locus was revealed for all strains of nodule bacteria of the Sinorhizobium genus. Phylogenetic analysis indicated that horizontal transfer of the mepA gene occurred in the course of evolution of bacterial tyrosinases. At the same time, the closely related members of nodule bacteria “acquired” this gene from different sources. Analysis of symbiotic properties of the Mep– mutants of the CA15-1 strain showed that melanin did not affect the ability to go into an efficient symbiosis with alfalfa. Most probably, it is important only at the stages of adaptation of the free-living cells in the environment.

AB - Identification and analysis of the melanin biosynthesis gene in the Sinorhizobium meliloti CA15-1 strain were carried out. Tn5 mutants, which lost the ability to synthesize melanin, were obtained. Molecular biological analysis of the mepA gene encoding tyrosinase was performed. In contrast to other members of the Rhizobiacea group, the identical structure of the mepA locus was revealed for all strains of nodule bacteria of the Sinorhizobium genus. Phylogenetic analysis indicated that horizontal transfer of the mepA gene occurred in the course of evolution of bacterial tyrosinases. At the same time, the closely related members of nodule bacteria “acquired” this gene from different sources. Analysis of symbiotic properties of the Mep– mutants of the CA15-1 strain showed that melanin did not affect the ability to go into an efficient symbiosis with alfalfa. Most probably, it is important only at the stages of adaptation of the free-living cells in the environment.

KW - horizontal gene transfer

KW - legume-rhizobium symbiosis

KW - melanin biosynthesis

KW - nodule bacteria

KW - tyrosinase

UR - http://www.scopus.com/inward/record.url?scp=85051865695&partnerID=8YFLogxK

UR - http://link.springer.com/10.1134/S1022795418080045

UR - http://www.mendeley.com/research/melanin-biosynthesis-gene-ca151-strain-alfalfa-nodule-bacteria-molecular-analysis-phylogeny

U2 - 10.1134/S1022795418080045

DO - 10.1134/S1022795418080045

M3 - Article

AN - SCOPUS:85051865695

VL - 54

SP - 925

EP - 932

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 8

ER -

ID: 36778271