Standard

The Genome of Paramecium caudatum: Analysis by Means of Pulsed Field Gel Electrophoresis. / Potekhin, A. A.; Rautian, M. S.; Brigge, T.

In: Russian Journal of Genetics, Vol. 35, No. 12, 01.12.1999, p. 1413-1420.

Research output: Contribution to journal › Article › peer-review

Harvard

Potekhin, AA, Rautian, MS & Brigge, T 1999, 'The Genome of Paramecium caudatum: Analysis by Means of Pulsed Field Gel Electrophoresis', Russian Journal of Genetics, vol. 35, no. 12, pp. 1413-1420.

APA

Potekhin, A. A., Rautian, M. S., & Brigge, T. (1999). The Genome of Paramecium caudatum: Analysis by Means of Pulsed Field Gel Electrophoresis. Russian Journal of Genetics, 35(12), 1413-1420.

Vancouver

Potekhin AA, Rautian MS, Brigge T. The Genome of Paramecium caudatum: Analysis by Means of Pulsed Field Gel Electrophoresis. Russian Journal of Genetics. 1999 Dec 1;35(12):1413-1420.

Author

Potekhin, A. A. ; Rautian, M. S. ; Brigge, T. / The Genome of Paramecium caudatum: Analysis by Means of Pulsed Field Gel Electrophoresis. In: Russian Journal of Genetics. 1999 ; Vol. 35, No. 12. pp. 1413-1420.

BibTeX

@article{43d61f1bccea46a4929bed0fe2e830a5,
title = "The Genome of Paramecium caudatum: Analysis by Means of Pulsed Field Gel Electrophoresis",
abstract = "Electrokaryotypes of eleven clones of the ciliated protozoan Paramecium caudatum were obtained by means of pulsed field gel electrophoresis (PFGE) and analyzed using the densitometry and Southern blot hybridization techniques. The main characteristics of the spectra of macronuclear DNA fragments from different clones were identical. Southern hybridization with the sequences complementary to the telomerase RNA component and the calmodulin gene showed that these genes were located in macronuclear DNA fragments of similar size in all clones studied. The interclone polymorphism was demonstrated only for the number and position of the bands corresponding to hyperamplified molecules. Quantitative analysis of the macronuclear DNA spectra from different P. caudatum clones was conducted. The total number of DNA molecules per macronuclear subgenome was estimated at 230 ± 40. The kinetic complexity of a macronuclear genome is discussed.",
author = "Potekhin, {A. A.} and Rautian, {M. S.} and T. Brigge",
year = "1999",
month = dec,
day = "1",
language = "English",
volume = "35",
pages = "1413--1420",
journal = "Russian Journal of Genetics",
issn = "1022-7954",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "12",

}

RIS

TY - JOUR

T1 - The Genome of Paramecium caudatum: Analysis by Means of Pulsed Field Gel Electrophoresis

AU - Potekhin, A. A.

AU - Rautian, M. S.

AU - Brigge, T.

PY - 1999/12/1

Y1 - 1999/12/1

N2 - Electrokaryotypes of eleven clones of the ciliated protozoan Paramecium caudatum were obtained by means of pulsed field gel electrophoresis (PFGE) and analyzed using the densitometry and Southern blot hybridization techniques. The main characteristics of the spectra of macronuclear DNA fragments from different clones were identical. Southern hybridization with the sequences complementary to the telomerase RNA component and the calmodulin gene showed that these genes were located in macronuclear DNA fragments of similar size in all clones studied. The interclone polymorphism was demonstrated only for the number and position of the bands corresponding to hyperamplified molecules. Quantitative analysis of the macronuclear DNA spectra from different P. caudatum clones was conducted. The total number of DNA molecules per macronuclear subgenome was estimated at 230 ± 40. The kinetic complexity of a macronuclear genome is discussed.

AB - Electrokaryotypes of eleven clones of the ciliated protozoan Paramecium caudatum were obtained by means of pulsed field gel electrophoresis (PFGE) and analyzed using the densitometry and Southern blot hybridization techniques. The main characteristics of the spectra of macronuclear DNA fragments from different clones were identical. Southern hybridization with the sequences complementary to the telomerase RNA component and the calmodulin gene showed that these genes were located in macronuclear DNA fragments of similar size in all clones studied. The interclone polymorphism was demonstrated only for the number and position of the bands corresponding to hyperamplified molecules. Quantitative analysis of the macronuclear DNA spectra from different P. caudatum clones was conducted. The total number of DNA molecules per macronuclear subgenome was estimated at 230 ± 40. The kinetic complexity of a macronuclear genome is discussed.

UR - http://www.scopus.com/inward/record.url?scp=0005598962&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0005598962

VL - 35

SP - 1413

EP - 1420

JO - Russian Journal of Genetics

JF - Russian Journal of Genetics

SN - 1022-7954

IS - 12

ER -

ID: 41518753