Standard

The genome of paramecium caudatum : Analysis by means of pulsed field gel electrophoresis. / Potekhin, A. A.; Rautian, M. S.; Brigge, T.

In: Genetika, Vol. 35, No. 12, 01.12.1999, p. 1643-1650.

Research output: Contribution to journal › Article › peer-review

Harvard

Potekhin, AA, Rautian, MS & Brigge, T 1999, 'The genome of paramecium caudatum: Analysis by means of pulsed field gel electrophoresis', Genetika, vol. 35, no. 12, pp. 1643-1650.

APA

Potekhin, A. A., Rautian, M. S., & Brigge, T. (1999). The genome of paramecium caudatum: Analysis by means of pulsed field gel electrophoresis. Genetika, 35(12), 1643-1650.

Vancouver

Potekhin AA, Rautian MS, Brigge T. The genome of paramecium caudatum: Analysis by means of pulsed field gel electrophoresis. Genetika. 1999 Dec 1;35(12):1643-1650.

Author

Potekhin, A. A. ; Rautian, M. S. ; Brigge, T. / The genome of paramecium caudatum : Analysis by means of pulsed field gel electrophoresis. In: Genetika. 1999 ; Vol. 35, No. 12. pp. 1643-1650.

BibTeX

@article{1bc0a261861c40a39ea47b083cf70de6,
title = "The genome of paramecium caudatum: Analysis by means of pulsed field gel electrophoresis",
abstract = "Electrokaryotypes of eleven clones of the ciliated protozoan Paramecium caudatum were obtained by means of pulsed field gel electrophoresis (PFGE) and analyzed using the densitometry and Southern blot hybridization techniques. The main characteristics of the spectra of macronuclear DN A fragments from different clones were identical. Southern hybridization with the sequences complementary to the telomerase RNA component and calmodulin genes showed that these genes were located in macronuclear DNA fragments of similar size in all clones studied. The interclone polymorphism was demonstrated only for the number and position of the bands corresponding to hyperamplified molecules. Quantitative analysis of the macronuclear DNA spectra from different P. caudatum clones was conducted. The total number of DNA molecules per macronuclear subgenome was estimated at 230 ±40. The kinetic complexity of a macronuclear genome is discussed.",
author = "Potekhin, {A. A.} and Rautian, {M. S.} and T. Brigge",
year = "1999",
month = dec,
day = "1",
language = "русский",
volume = "35",
pages = "1643--1650",
journal = "ГЕНЕТИКА",
issn = "0016-6758",
publisher = "Международная книга",
number = "12",

}

RIS

TY - JOUR

T1 - The genome of paramecium caudatum

T2 - Analysis by means of pulsed field gel electrophoresis

AU - Potekhin, A. A.

AU - Rautian, M. S.

AU - Brigge, T.

PY - 1999/12/1

Y1 - 1999/12/1

N2 - Electrokaryotypes of eleven clones of the ciliated protozoan Paramecium caudatum were obtained by means of pulsed field gel electrophoresis (PFGE) and analyzed using the densitometry and Southern blot hybridization techniques. The main characteristics of the spectra of macronuclear DN A fragments from different clones were identical. Southern hybridization with the sequences complementary to the telomerase RNA component and calmodulin genes showed that these genes were located in macronuclear DNA fragments of similar size in all clones studied. The interclone polymorphism was demonstrated only for the number and position of the bands corresponding to hyperamplified molecules. Quantitative analysis of the macronuclear DNA spectra from different P. caudatum clones was conducted. The total number of DNA molecules per macronuclear subgenome was estimated at 230 ±40. The kinetic complexity of a macronuclear genome is discussed.

AB - Electrokaryotypes of eleven clones of the ciliated protozoan Paramecium caudatum were obtained by means of pulsed field gel electrophoresis (PFGE) and analyzed using the densitometry and Southern blot hybridization techniques. The main characteristics of the spectra of macronuclear DN A fragments from different clones were identical. Southern hybridization with the sequences complementary to the telomerase RNA component and calmodulin genes showed that these genes were located in macronuclear DNA fragments of similar size in all clones studied. The interclone polymorphism was demonstrated only for the number and position of the bands corresponding to hyperamplified molecules. Quantitative analysis of the macronuclear DNA spectra from different P. caudatum clones was conducted. The total number of DNA molecules per macronuclear subgenome was estimated at 230 ±40. The kinetic complexity of a macronuclear genome is discussed.

UR - http://www.scopus.com/inward/record.url?scp=33746140613&partnerID=8YFLogxK

M3 - статья

AN - SCOPUS:33746140613

VL - 35

SP - 1643

EP - 1650

JO - ГЕНЕТИКА

JF - ГЕНЕТИКА

SN - 0016-6758

IS - 12

ER -

ID: 39839514