The first case of microsporidiosis in Paramecium

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The first case of microsporidiosis in Paramecium. / Yakovleva, Yulia ; Nassonova, Elena ; Lebedeva, Natalia ; Lanzoni, Olivia; Petroni, G.; Potekhin, Alexey ; Sabaneyeva, Elena .

In: Parasitology, Vol. 147, No. 9, 01.08.2020, p. 957-971.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{73da9173b10f46c383982d3fbffb73d7,

title = "The first case of microsporidiosis in Paramecium",

abstract = "A new microsporidian species,Globosporidium parameciigen. nov., sp. nov., fromParamecium primaureliais described on the basis of morphology, fine structure, and SSU rRNA gene sequence. This is the first case of microsporidiosis inParameciumreported so far. All observed stages of the life cycle are monokaryotic. The parasites develop in the cytoplasm, at least some part of the population in endoplasmic reticulum and its derivates. Meronts divide by binary fission. Sporogonial plasmodium divides by rosette-like budding. Early sporoblasts demonstrate a well-developed exospore forming blister-like structures. Spores with distinctive spherical shape are dimorphic in size (3.7 +/- 0.2 and 1.9 +/- 0.2 mu m). Both types of spores are characterized by a thin endospore, a short isofilar polar tube making one incomplete coil, a bipartite polaroplast, and a large posterior vacuole. Experimental infection was successful for 5 of 10 tested strains of theParamecium aureliaspecies complex. All susceptible strains belong to closely relatedP. primaureliaandP. pentaureliaspecies. Phylogenetic analysis placed the new species in the Clade 4 of Microsporidia and revealed its close relationship toEuplotespora binucleata(a microsporidium from the ciliateEuplotes woodruffi), toHelmichia lacustrisandMrazekia macrocyclopis, microsporidia from aquatic invertebrates.",

keywords = "микроспоридии, инфузории, симбиоз, Ciliate, Globosporidium, Paramecium, host specificity, intracellular parasitism, microsporidia, phylogeny, BIODIVERSITY, INFECTIONS, PARAMECIUM, MOLECULAR PHYLOGENY, CILIOPHORA, TRANSMISSION, SEQUENCE ALIGNMENT, ULTRASTRUCTURE, SP N. MICROSPORIDIA, PARASITE",

author = "Yulia Yakovleva and Elena Nassonova and Natalia Lebedeva and Olivia Lanzoni and G. Petroni and Alexey Potekhin and Elena Sabaneyeva",

note = "Funding Information: This work was supported by the Russian Foundation for Basic Research grant 18-04-00562a to ES (light, fluorescent and electron microscopy, experimental infections), Russian Science Foundation grant 19-74-20136 to EN (SSU sequencing and molecular phylogeny) and Russian Science Foundation grant 16-14-10157 to AP (identification of the host species). This study utilized equipment of the Saint Petersburg University Science Park (Resource Centre {\textquoteleft}Culture Collection of Microorganisms{\textquoteright} (RC CCM), and Core Facility Centers {\textquoteleft}Development of Molecular and Cell technologies{\textquoteright} and{\textquoteleft}Microscopy and Microanalysis{\textquoteright}).",

year = "2020",

month = aug,

day = "1",

doi = "10.1017/S0031182020000633",

language = "English",

volume = "147",

pages = "957--971",

journal = "Parasitology",

issn = "0031-1820",

publisher = "Cambridge University Press",

number = "9",

}

RIS

TY - JOUR

T1 - The first case of microsporidiosis in Paramecium

AU - Yakovleva, Yulia

AU - Nassonova, Elena

AU - Lebedeva, Natalia

AU - Lanzoni, Olivia

AU - Petroni, G.

AU - Potekhin, Alexey

AU - Sabaneyeva, Elena

N1 - Funding Information: This work was supported by the Russian Foundation for Basic Research grant 18-04-00562a to ES (light, fluorescent and electron microscopy, experimental infections), Russian Science Foundation grant 19-74-20136 to EN (SSU sequencing and molecular phylogeny) and Russian Science Foundation grant 16-14-10157 to AP (identification of the host species). This study utilized equipment of the Saint Petersburg University Science Park (Resource Centre ‘Culture Collection of Microorganisms’ (RC CCM), and Core Facility Centers ‘Development of Molecular and Cell technologies’ and‘Microscopy and Microanalysis’).

PY - 2020/8/1

Y1 - 2020/8/1

N2 - A new microsporidian species,Globosporidium parameciigen. nov., sp. nov., fromParamecium primaureliais described on the basis of morphology, fine structure, and SSU rRNA gene sequence. This is the first case of microsporidiosis inParameciumreported so far. All observed stages of the life cycle are monokaryotic. The parasites develop in the cytoplasm, at least some part of the population in endoplasmic reticulum and its derivates. Meronts divide by binary fission. Sporogonial plasmodium divides by rosette-like budding. Early sporoblasts demonstrate a well-developed exospore forming blister-like structures. Spores with distinctive spherical shape are dimorphic in size (3.7 +/- 0.2 and 1.9 +/- 0.2 mu m). Both types of spores are characterized by a thin endospore, a short isofilar polar tube making one incomplete coil, a bipartite polaroplast, and a large posterior vacuole. Experimental infection was successful for 5 of 10 tested strains of theParamecium aureliaspecies complex. All susceptible strains belong to closely relatedP. primaureliaandP. pentaureliaspecies. Phylogenetic analysis placed the new species in the Clade 4 of Microsporidia and revealed its close relationship toEuplotespora binucleata(a microsporidium from the ciliateEuplotes woodruffi), toHelmichia lacustrisandMrazekia macrocyclopis, microsporidia from aquatic invertebrates.

AB - A new microsporidian species,Globosporidium parameciigen. nov., sp. nov., fromParamecium primaureliais described on the basis of morphology, fine structure, and SSU rRNA gene sequence. This is the first case of microsporidiosis inParameciumreported so far. All observed stages of the life cycle are monokaryotic. The parasites develop in the cytoplasm, at least some part of the population in endoplasmic reticulum and its derivates. Meronts divide by binary fission. Sporogonial plasmodium divides by rosette-like budding. Early sporoblasts demonstrate a well-developed exospore forming blister-like structures. Spores with distinctive spherical shape are dimorphic in size (3.7 +/- 0.2 and 1.9 +/- 0.2 mu m). Both types of spores are characterized by a thin endospore, a short isofilar polar tube making one incomplete coil, a bipartite polaroplast, and a large posterior vacuole. Experimental infection was successful for 5 of 10 tested strains of theParamecium aureliaspecies complex. All susceptible strains belong to closely relatedP. primaureliaandP. pentaureliaspecies. Phylogenetic analysis placed the new species in the Clade 4 of Microsporidia and revealed its close relationship toEuplotespora binucleata(a microsporidium from the ciliateEuplotes woodruffi), toHelmichia lacustrisandMrazekia macrocyclopis, microsporidia from aquatic invertebrates.

KW - микроспоридии, инфузории, симбиоз

KW - Ciliate

KW - Globosporidium

KW - Paramecium

KW - host specificity

KW - intracellular parasitism

KW - microsporidia

KW - phylogeny

KW - BIODIVERSITY

KW - INFECTIONS

KW - PARAMECIUM

KW - MOLECULAR PHYLOGENY

KW - CILIOPHORA

KW - TRANSMISSION

KW - SEQUENCE ALIGNMENT

KW - ULTRASTRUCTURE

KW - SP N. MICROSPORIDIA

KW - PARASITE

UR - http://www.scopus.com/inward/record.url?scp=85084646866&partnerID=8YFLogxK

U2 - 10.1017/S0031182020000633

DO - 10.1017/S0031182020000633

M3 - Article

VL - 147

SP - 957

EP - 971

JO - Parasitology

JF - Parasitology

SN - 0031-1820

IS - 9

ER -

ID: 53522830