The Enhancement of Metal-Binding Properties in Hemoglobin : The Role of Mild Damaging Factors. / Novikova, N. N.; Kovalchuk, M. V.; Yurieva, E. A.; Konovalov, O. V.; Stepina, N. D.; Rogachev, A. V.; Yalovega, G. E.; Kosmachevskaya, O. V.; Topunov, A. F.; Yakunin, S. N.
In: Journal of Physical Chemistry B, Vol. 123, No. 40, 10.10.2019, p. 8370-8377.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The Enhancement of Metal-Binding Properties in Hemoglobin
T2 - The Role of Mild Damaging Factors
AU - Novikova, N. N.
AU - Kovalchuk, M. V.
AU - Yurieva, E. A.
AU - Konovalov, O. V.
AU - Stepina, N. D.
AU - Rogachev, A. V.
AU - Yalovega, G. E.
AU - Kosmachevskaya, O. V.
AU - Topunov, A. F.
AU - Yakunin, S. N.
N1 - Publisher Copyright: © 2019 American Chemical Society.
PY - 2019/10/10
Y1 - 2019/10/10
N2 - X-ray studies revealed the considerable enhancement of metal-binding properties in human hemoglobin under exposure to mild damaging factors (in the presence of 0.09 M urea or upon heating for 30 min at 50 °C). Changes in the element composition of the hemoglobin monolayer, formed on the water subphase in the Langmuir trough, have been monitored in real time by the total external reflection X-ray fluorescence measurements. X-ray absorption spectroscopy has been applied to study the local environment of zinc ions bound on hemoglobin molecules. According to these data, each zinc ion is coordinated by four ligands, two of which are cysteine and histidine. The oxidative stress has been found to accelerate extensively the enhancement of metal-binding ability in protein. A two-stage mechanism has been proposed as a possible explanation of the observed phenomenon: First, in the presence of the mild damaging agents, protein molecules can undergo a transition from the native conformation to a more labile intermediate state that increases the accessibility of amino acid residues (in particular cysteine). At the second stage, oxidation of cysteine and the subsequent activation of cysteine SH groups can affect markedly the protein-metal interaction. The presented investigations provide a deeper insight into the pathogenesis of metabolic disorders that excessive concentrations of the endogenic toxicants might trigger in an organism.
AB - X-ray studies revealed the considerable enhancement of metal-binding properties in human hemoglobin under exposure to mild damaging factors (in the presence of 0.09 M urea or upon heating for 30 min at 50 °C). Changes in the element composition of the hemoglobin monolayer, formed on the water subphase in the Langmuir trough, have been monitored in real time by the total external reflection X-ray fluorescence measurements. X-ray absorption spectroscopy has been applied to study the local environment of zinc ions bound on hemoglobin molecules. According to these data, each zinc ion is coordinated by four ligands, two of which are cysteine and histidine. The oxidative stress has been found to accelerate extensively the enhancement of metal-binding ability in protein. A two-stage mechanism has been proposed as a possible explanation of the observed phenomenon: First, in the presence of the mild damaging agents, protein molecules can undergo a transition from the native conformation to a more labile intermediate state that increases the accessibility of amino acid residues (in particular cysteine). At the second stage, oxidation of cysteine and the subsequent activation of cysteine SH groups can affect markedly the protein-metal interaction. The presented investigations provide a deeper insight into the pathogenesis of metabolic disorders that excessive concentrations of the endogenic toxicants might trigger in an organism.
UR - http://www.scopus.com/inward/record.url?scp=85073124361&partnerID=8YFLogxK
U2 - 10.1021/acs.jpcb.9b06571
DO - 10.1021/acs.jpcb.9b06571
M3 - Article
C2 - 31513409
AN - SCOPUS:85073124361
VL - 123
SP - 8370
EP - 8377
JO - Journal of Physical Chemistry B
JF - Journal of Physical Chemistry B
SN - 1520-6106
IS - 40
ER -
ID: 88198625