Research output: Contribution to journal › Article › peer-review
The Effects of Severe Hypobaric Hypoxia and Inhibition of Hypoxia-Inducible Factor-1 (HIF-1) on Biomarkers of Cardiac and Skeletal Muscle Injury in Rats. / Fedorov, D. A. ; Frolova, M. Yu. ; Krasovskaya, I. E. ; Kuleva, N. V. .
In: Biophysics (Russian Federation), Vol. 64, No. 5, 2019, p. 808-811.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The Effects of Severe Hypobaric Hypoxia and Inhibition of Hypoxia-Inducible Factor-1 (HIF-1) on Biomarkers of Cardiac and Skeletal Muscle Injury in Rats
AU - Fedorov, D. A.
AU - Frolova, M. Yu.
AU - Krasovskaya, I. E.
AU - Kuleva, N. V.
N1 - Fedorov, D.A., Frolova, M.Y., Krasovskaya, I.E. et al. The Effects of Severe Hypobaric Hypoxia and Inhibition of Hypoxia-Inducible Factor-1 (HIF-1) on Biomarkers of Cardiac and Skeletal Muscle Injury in Rats. BIOPHYSICS 64, 808–811 (2019) doi:10.1134/S000635091905004X
PY - 2019
Y1 - 2019
N2 - The goal of the present study was to investigate the molecular mechanisms that underlie heart and skeletal muscle damage in male Wistar rats weighing 200–250 g in response to a 3-h exposure to 180 mm Hg (5% O2) in the model of severe hypobaric hypoxia. It has been demonstrated that the level of the cardiac biomarker troponin I in the blood plasma of rats exposed to severe hypobaric hypoxia for 3 h increased significantly compared to the control group, indicating myocardial injury. At the same time, the administration of the HIF-1α transcription factor inhibitor did not affect the plasma level of troponin I. In contrast, the release of the non-specific biomarker myoglobin into the bloodstream did not increase in response to hypoxia compared to the control animals. In addition, 24 h after the exposure to severe hypobaric hypoxia the serum myoglobin level was significantly lower in animals administered with the HIF-1α inhibitor topotecan than in rats that did not receive topotecan. Therefore, it may be assumed that the inhibition of the HIF-1α transcription factor 10 min before exposure to severe hypobaric hypoxia reduces skeletal muscle damage. The mechanisms that affect the adaptation of heart and skeletal muscles to hypoxia are discussed.
AB - The goal of the present study was to investigate the molecular mechanisms that underlie heart and skeletal muscle damage in male Wistar rats weighing 200–250 g in response to a 3-h exposure to 180 mm Hg (5% O2) in the model of severe hypobaric hypoxia. It has been demonstrated that the level of the cardiac biomarker troponin I in the blood plasma of rats exposed to severe hypobaric hypoxia for 3 h increased significantly compared to the control group, indicating myocardial injury. At the same time, the administration of the HIF-1α transcription factor inhibitor did not affect the plasma level of troponin I. In contrast, the release of the non-specific biomarker myoglobin into the bloodstream did not increase in response to hypoxia compared to the control animals. In addition, 24 h after the exposure to severe hypobaric hypoxia the serum myoglobin level was significantly lower in animals administered with the HIF-1α inhibitor topotecan than in rats that did not receive topotecan. Therefore, it may be assumed that the inhibition of the HIF-1α transcription factor 10 min before exposure to severe hypobaric hypoxia reduces skeletal muscle damage. The mechanisms that affect the adaptation of heart and skeletal muscles to hypoxia are discussed.
KW - hypoxia, myoglobin, troponin I, topotecan,hypoxia-inducible factor 1-alpha (HIF-1α), myocardium
KW - myoglobin
UR - http://link.springer.com/article/10.1134/S000635091905004X
M3 - Article
VL - 64
SP - 808
EP - 811
JO - Biophysics (Russian Federation)
JF - Biophysics (Russian Federation)
SN - 0006-3509
IS - 5
ER -
ID: 49229330