TY - JOUR

T1 - The effects of chemical etching and ultra-fine grain structure of titanium on mg-63 cells response

AU - Nazarov, Denis

AU - Zemtsova, Elena

AU - Smirnov, Vladimir

AU - Mitrofanov, Ilya

AU - Maximov, Maxim

AU - Yudintceva, Natalia

AU - Shevtsov, Maxim

N1 - Publisher Copyright: © 2021 by the authors. Licensee MDPI, Basel, Switzerland.

PY - 2021/3/19

Y1 - 2021/3/19

N2 - In this work, we study the influence of the surface properties of ultrafine grained (UFG) and coarse grained (CG) titanium on the morphology, viability, proliferation and differentiation of osteoblast-like MG-63 cells. Wet chemical etching in H2SO4/H2O2 and NH4OH/H2O2 solutions was used for producing surfaces with varying morphology, topography, composition and wettability. The topography and morphology have been studied by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The composition was determined by time of flight mass-spectrometry (TOF-SIMS) and X-ray photoelectron spectroscopy (XPS). The results showed that it is possible to obtain samples with different compositions, hydrophilicity, topography and nanoscale or/and microscale structures by changing the etching time and the type of etching solution. It was found that developed topography and morphology can improve spreading and proliferation rate of MG-63 cells. A significant advantage of the samples of the UFG series in comparison with CG in adhesion, proliferation at later stages of cultivation (7 days), higher alkaline phosphatase (ALP) activity and faster achievement of its maximum values was found. However, there is no clear benefit of the UFG series on osteopontin (OPN) expression. All studied samples showed no cytotoxicity towards MG-63 cells and promoted their osteogenic differentiation.

AB - In this work, we study the influence of the surface properties of ultrafine grained (UFG) and coarse grained (CG) titanium on the morphology, viability, proliferation and differentiation of osteoblast-like MG-63 cells. Wet chemical etching in H2SO4/H2O2 and NH4OH/H2O2 solutions was used for producing surfaces with varying morphology, topography, composition and wettability. The topography and morphology have been studied by scanning electron microscopy (SEM) and atomic force microscopy (AFM). The composition was determined by time of flight mass-spectrometry (TOF-SIMS) and X-ray photoelectron spectroscopy (XPS). The results showed that it is possible to obtain samples with different compositions, hydrophilicity, topography and nanoscale or/and microscale structures by changing the etching time and the type of etching solution. It was found that developed topography and morphology can improve spreading and proliferation rate of MG-63 cells. A significant advantage of the samples of the UFG series in comparison with CG in adhesion, proliferation at later stages of cultivation (7 days), higher alkaline phosphatase (ALP) activity and faster achievement of its maximum values was found. However, there is no clear benefit of the UFG series on osteopontin (OPN) expression. All studied samples showed no cytotoxicity towards MG-63 cells and promoted their osteogenic differentiation.

KW - titanium

KW - Ultrafine grained materials

KW - chemical etching

KW - surface science

KW - osteoblast response

KW - Osteoblast response

KW - Titanium

KW - Surface science

KW - Chemical etching

UR - http://www.scopus.com/inward/record.url?scp=85102691422&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/641eb933-f56e-3359-be13-20c5ea7ec754/

U2 - 10.3390/met11030510

DO - 10.3390/met11030510

M3 - Article

VL - 11

SP - 1

EP - 20

JO - Metals

JF - Metals

SN - 2075-4701

IS - 3

M1 - 510

ER -