Research output: Contribution to journal › Article › peer-review
The effect of hypercaloric diet and Quercetin on the full-transcriptome liver tissue profile of Zucker-LEPR fa rats. / Trusov, Nikita V.; Apryatin, Sergey A.; Gorbachev, Aleksey Yu; Naumov, Vladimir A.; Mzhelskaya, Kristina V.; Gmoshinski, Ivan V.
In: Problemy Endokrinologii, Vol. 64, No. 6, 01.01.2018, p. 371-382.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The effect of hypercaloric diet and Quercetin on the full-transcriptome liver tissue profile of Zucker-LEPR fa rats
AU - Trusov, Nikita V.
AU - Apryatin, Sergey A.
AU - Gorbachev, Aleksey Yu
AU - Naumov, Vladimir A.
AU - Mzhelskaya, Kristina V.
AU - Gmoshinski, Ivan V.
PY - 2018/1/1
Y1 - 2018/1/1
N2 - BACKGROUND: The peptidic hormone leptin (Lep) occupies a central place in the control of energy homeostasis and body weight in mammals. A convenient model for studying the role of impaired Lep reception is the Zucker-LEPR fa rats, which carry a mutation in the homozygote of the LEPR gene. Quercetin (Q; 3.3 ‘, 4', 5.7-pentahydroxyflavone) is currently being considered as one of the promising biologically active substances, which allows to correct metabolic disorders in obesity and metabolic syndrome. AIM: to study changes in the expression of genes in liver tissue of rats with impaired receptivity of Lep under the influence of high-fat and high-carbohydrate diet (HFCR) or/and Q supplementation. MATERIAL AND METHODS. 4 groups of six male Zucker-LEPR fa male rats were used in experiment. Within 61 days the animals of the 1st group (control) received a balanced semi-synthetic diet, the second group received the same diet with the addition of quercetin in a dose of 50 mg/kg of body weight, the third group — the HFCR (30% fat by dry weight and 20% fructose instead of water), the 4th group is the same diet and supplement of quercetin. Full transcriptional profiling of liver tissue was performed on microchips from the Gene Expression Hybridization Kit (Agilent Technologies), a real-time polymerase chain reaction combined with reverse transcription (RT-PCR) was performed for liver transcripts of Crot, FTO, NpY, Prdx1, Prom1, Ugt2b37 and GAPDH genes contained in liver tissue. RESULTS. It was shown that feeding of Zucker-LEPR fa rats with Q and/or HFCR led to significant changes in the level of transcription of 1604 genes in liver tissue, from which the effect of quercetin proper was manifested for 1396 genes. Changes were more pronounced in the transcriptome of liver tissue caused by HFCR, than caused by the addition of Q against the background of a standard diet. Q influenced the expression of genes responsible for xenobiotic detoxification processes (UGT2b37), redox homeostasis (Prdx1), beta-oxidation of fatty acids (Crot), and central mechanisms affecting hunger and satiety (NpY), and potentiated, or abolished the effects of HFCR against a number of other functionally important genes. Bioinformatic analysis revealed the influence of HFCR and/or Q on 23 metabolic pathways (KEGGS), of which 7 (the metabolism of steroids, arachidonic and linoleic acids, retinoids, drugs and xenobiotics (due to cytochrome P-450), bile secretion) were affected in all experimental groups. CONCLUSIONS. Changes in the transcriptome of the liver of Zucker-LEPR fa rats, caused by consumption of HFCR and/or Q, were consistent with experimental data on changes in short-term memory, anxiety and mineral metabolism in these animals.
AB - BACKGROUND: The peptidic hormone leptin (Lep) occupies a central place in the control of energy homeostasis and body weight in mammals. A convenient model for studying the role of impaired Lep reception is the Zucker-LEPR fa rats, which carry a mutation in the homozygote of the LEPR gene. Quercetin (Q; 3.3 ‘, 4', 5.7-pentahydroxyflavone) is currently being considered as one of the promising biologically active substances, which allows to correct metabolic disorders in obesity and metabolic syndrome. AIM: to study changes in the expression of genes in liver tissue of rats with impaired receptivity of Lep under the influence of high-fat and high-carbohydrate diet (HFCR) or/and Q supplementation. MATERIAL AND METHODS. 4 groups of six male Zucker-LEPR fa male rats were used in experiment. Within 61 days the animals of the 1st group (control) received a balanced semi-synthetic diet, the second group received the same diet with the addition of quercetin in a dose of 50 mg/kg of body weight, the third group — the HFCR (30% fat by dry weight and 20% fructose instead of water), the 4th group is the same diet and supplement of quercetin. Full transcriptional profiling of liver tissue was performed on microchips from the Gene Expression Hybridization Kit (Agilent Technologies), a real-time polymerase chain reaction combined with reverse transcription (RT-PCR) was performed for liver transcripts of Crot, FTO, NpY, Prdx1, Prom1, Ugt2b37 and GAPDH genes contained in liver tissue. RESULTS. It was shown that feeding of Zucker-LEPR fa rats with Q and/or HFCR led to significant changes in the level of transcription of 1604 genes in liver tissue, from which the effect of quercetin proper was manifested for 1396 genes. Changes were more pronounced in the transcriptome of liver tissue caused by HFCR, than caused by the addition of Q against the background of a standard diet. Q influenced the expression of genes responsible for xenobiotic detoxification processes (UGT2b37), redox homeostasis (Prdx1), beta-oxidation of fatty acids (Crot), and central mechanisms affecting hunger and satiety (NpY), and potentiated, or abolished the effects of HFCR against a number of other functionally important genes. Bioinformatic analysis revealed the influence of HFCR and/or Q on 23 metabolic pathways (KEGGS), of which 7 (the metabolism of steroids, arachidonic and linoleic acids, retinoids, drugs and xenobiotics (due to cytochrome P-450), bile secretion) were affected in all experimental groups. CONCLUSIONS. Changes in the transcriptome of the liver of Zucker-LEPR fa rats, caused by consumption of HFCR and/or Q, were consistent with experimental data on changes in short-term memory, anxiety and mineral metabolism in these animals.
KW - Leptin
KW - Liver
KW - Metabolic networks
KW - Obesity
KW - Pathways
KW - Rats
KW - Transcriptome
UR - http://www.scopus.com/inward/record.url?scp=85063898811&partnerID=8YFLogxK
U2 - 10.14341/probl9936
DO - 10.14341/probl9936
M3 - Article
AN - SCOPUS:85063898811
VL - 64
SP - 371
EP - 382
JO - ПРОБЛЕМЫ ЭНДОКРИНОЛОГИИ
JF - ПРОБЛЕМЫ ЭНДОКРИНОЛОГИИ
SN - 0375-9660
IS - 6
ER -
ID: 115017140