Research output: Contribution to journal › Article › peer-review
The effect of buffer concentration on the dynamic/equilibrium surface tension of bovine serum albumin solution. / Lin, Shi-Yow; Hussain, Siam; Hung, Sheng-Hsiang ; Tseng, Wen-Chi; Носков, Борис Анатольевич; Tsay, Ruey-Yug .
In: Journal of Molecular Liquids, Vol. 381, 121837, 01.07.2023.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The effect of buffer concentration on the dynamic/equilibrium surface tension of bovine serum albumin solution
AU - Lin, Shi-Yow
AU - Hussain, Siam
AU - Hung, Sheng-Hsiang
AU - Tseng, Wen-Chi
AU - Носков, Борис Анатольевич
AU - Tsay, Ruey-Yug
PY - 2023/7/1
Y1 - 2023/7/1
N2 - The effect of phosphate buffer (Na2HPO4 and NaH2PO4) concentration on the dynamic and equilibrium surface tension (ST) of bovine serum albumin (BSA) solutions was examined in this study. A pendant bubble tensiometer was utilized for measuring the ST relaxation of BSA solutions in varying buffer concentration (0, 0.01–300 mM). The data showed: (i) an initial increase in buffer concentration (Cbuff < 0.3 mM) resulted in a slower ST relaxation and a notable rise in the equilibrium ST; (ii) a further rise in buffer concentration (Cbuff > 0.3 mM) resulted in a faster ST relaxation and a progressive decrease in the equilibrium ST; and (iii) despite an increase in BSA concentration from 2 to 15 (10−10 mol/cm3), the dynamic ST of BSA solutions always relaxed the slowest at Cbuff = 0.3 mM. The equilibrium ST of BSA solutions in 10 and 44 mM buffer [C = 0.1–15 (10−10 mol/cm3)] was also obtained and the data confirmed the near-constant equilibrium ST of purely aqueous BSA solutions [Le et al., J.Mol.Liq (2022) 118305] to not be an anomalous behavior but, observable irrespective of the solvent formulation.
AB - The effect of phosphate buffer (Na2HPO4 and NaH2PO4) concentration on the dynamic and equilibrium surface tension (ST) of bovine serum albumin (BSA) solutions was examined in this study. A pendant bubble tensiometer was utilized for measuring the ST relaxation of BSA solutions in varying buffer concentration (0, 0.01–300 mM). The data showed: (i) an initial increase in buffer concentration (Cbuff < 0.3 mM) resulted in a slower ST relaxation and a notable rise in the equilibrium ST; (ii) a further rise in buffer concentration (Cbuff > 0.3 mM) resulted in a faster ST relaxation and a progressive decrease in the equilibrium ST; and (iii) despite an increase in BSA concentration from 2 to 15 (10−10 mol/cm3), the dynamic ST of BSA solutions always relaxed the slowest at Cbuff = 0.3 mM. The equilibrium ST of BSA solutions in 10 and 44 mM buffer [C = 0.1–15 (10−10 mol/cm3)] was also obtained and the data confirmed the near-constant equilibrium ST of purely aqueous BSA solutions [Le et al., J.Mol.Liq (2022) 118305] to not be an anomalous behavior but, observable irrespective of the solvent formulation.
KW - Bovine serum albumin
KW - Buffer concentration
KW - Dynamic surface tension
KW - Equilibrium surface tension
KW - Pendant bubble
KW - Phosphate buffer
UR - https://www.mendeley.com/catalogue/806359fd-2649-3f4e-b184-124b0e532e1a/
U2 - 10.1016/j.molliq.2023.121837
DO - 10.1016/j.molliq.2023.121837
M3 - Article
VL - 381
JO - Journal of Molecular Liquids
JF - Journal of Molecular Liquids
SN - 0167-7322
M1 - 121837
ER -
ID: 108682454