Research output: Contribution to journal › Article › peer-review
The core microbiome of sessile ciliate Stentor coeruleus is not shaped by the environment. / Lanzoni, Olivia; Plotnikov, Andrey; Khlopko, Yuri; Munz, Giulio; Petroni, Giulio; Potekhin, Alexey.
In: Scientific Reports, Vol. 9, No. 1, 11356, 01.12.2019.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - The core microbiome of sessile ciliate Stentor coeruleus is not shaped by the environment
AU - Lanzoni, Olivia
AU - Plotnikov, Andrey
AU - Khlopko, Yuri
AU - Munz, Giulio
AU - Petroni, Giulio
AU - Potekhin, Alexey
N1 - Publisher Copyright: © 2019, The Author(s).
PY - 2019/12/1
Y1 - 2019/12/1
N2 - Microbiomes of multicellular organisms are one of the hottest topics in microbiology and physiology, while only few studies addressed bacterial communities associated with protists. Protists are widespread in all environments and can be colonized by plethora of different bacteria, including also human pathogens. The aim of this study was to characterize the prokaryotic community associated with the sessile ciliate Stentor coeruleus. 16S rRNA gene metabarcoding was performed on single cells of S. coeruleus and on their environment, water from the sewage stream. Our results showed that the prokaryotic community composition differed significantly between Stentor cells and their environment. The core microbiome common for all ciliate specimens analyzed could be defined, and it was composed mainly by representatives of bacterial genera which include also potential human pathogens and commensals, such as Neisseria, Streptococcus, Capnocytophaga, Porphyromonas. Numerous 16S rRNA gene contigs belonged to endosymbiont “Candidatus Megaira polyxenophila”. Our data suggest that each ciliate cell can be considered as an ecological microniche harboring diverse prokaryotic organisms. Possible benefits for persistence and transmission in nature for bacteria associated with protists are discussed. Our results support the hypothesis that ciliates attract potentially pathogenic bacteria and play the role of natural reservoirs for them.
AB - Microbiomes of multicellular organisms are one of the hottest topics in microbiology and physiology, while only few studies addressed bacterial communities associated with protists. Protists are widespread in all environments and can be colonized by plethora of different bacteria, including also human pathogens. The aim of this study was to characterize the prokaryotic community associated with the sessile ciliate Stentor coeruleus. 16S rRNA gene metabarcoding was performed on single cells of S. coeruleus and on their environment, water from the sewage stream. Our results showed that the prokaryotic community composition differed significantly between Stentor cells and their environment. The core microbiome common for all ciliate specimens analyzed could be defined, and it was composed mainly by representatives of bacterial genera which include also potential human pathogens and commensals, such as Neisseria, Streptococcus, Capnocytophaga, Porphyromonas. Numerous 16S rRNA gene contigs belonged to endosymbiont “Candidatus Megaira polyxenophila”. Our data suggest that each ciliate cell can be considered as an ecological microniche harboring diverse prokaryotic organisms. Possible benefits for persistence and transmission in nature for bacteria associated with protists are discussed. Our results support the hypothesis that ciliates attract potentially pathogenic bacteria and play the role of natural reservoirs for them.
UR - https://www.nature.com/articles/s41598-019-47701-8
UR - http://www.scopus.com/inward/record.url?scp=85070300001&partnerID=8YFLogxK
U2 - 10.1038/s41598-019-47701-8
DO - 10.1038/s41598-019-47701-8
M3 - Article
VL - 9
JO - Scientific Reports
JF - Scientific Reports
SN - 2045-2322
IS - 1
M1 - 11356
ER -
ID: 44461968