The karyotype of meadow fescue Festuca pratensis Huds. (2 n=14) has been studied by means of fluorescence banding techniques. At-enriched heterochromatic bands were revealed in the metaphase chromosomes 1-6. A single GC-rich band was associated with the chromosome 2 secondary constriction. Delay of condensation of the heterochromatic regions was discovered. The heterochromatic bands reported are markers which allow identification of all meadow fescue chromosomes.