In this work, a homogeneous liquid-liquid microextraction approach based on temperature-responsive deep eutectic solvent for pretreatment of beverages was developed for the first time. The procedure assumed the heating of the homogeneous solution, consisting of sample, precursors of temperature-responsive deep eutectic solvent (amphiphilic amine and carboxylic acid) and salting-out agent in a disposable polyethylene pipette. The heating of extraction system induced the change of acid-base properties of the precursors, and thus it attributed to in situ formation of microdroplets of deep eutectic solvent in beverage sample. The significant difference in densities of aqueous sample phase and the extraction solvent allowed to obtain spontaneous phase separation, while the presence of a thin capillary on top of the pipette facilitated microvolume sampling of the resulting extract. The approach was utilized for the determination of bisphenols (bisphenol A, bisphenol C, bisphenol E and bisphenol F) in beverages by high-performance liquid chromatography with fluorometric detection. The temperature-responsive deep eutectic solvent based on lidocaine and heptanoic acid provided the highest preconcentration of analytes (enrichment factor – 22). Under optimal conditions, the RSD in terms of inter-day precision was < 11 %, limits of detection for bisphenols were 0.01–0.03 μg L−1.