Telomere-binding TRF2/MTBP localization during mouse spermatogenesis and cell cycle of the mouse cells L929. / Dolnik, AV; Kuznetsova, IS; Voronin, AP; Podgornaya, OI.
In: Journal of Anti-Aging Medicine, Vol. 6, No. 2, 2003, p. 107-121.Research output: Contribution to journal › Article
}
TY - JOUR
T1 - Telomere-binding TRF2/MTBP localization during mouse spermatogenesis and cell cycle of the mouse cells L929.
AU - Dolnik, AV
AU - Kuznetsova, IS
AU - Voronin, AP
AU - Podgornaya, OI.
PY - 2003
Y1 - 2003
N2 - Observations of the organization and distribution of telomeres (Tel) in somatic tissues still remain controversial. The Tel topography revealed by modern microscopy shows them to be associated with the nuclear envelope (NE) in a wide variety of eukaryotic cells, although not at the Rabl orientation (peripheral position at one pole of the nucleus at prophase). We used two cell types that have different nuclear architectures. The cell line L929 shows lack of any rigid Tel architecture in the nucleus. In contrast, spermatozoa have a precise architecture established during spermiogenesis. We observed Tel and membrane Tel binding protein (MTBP/TRF2) position by immunoFISH in L929 cells and by immunofluorescence and immunogold electron microscopy, using antibodies against Membrane Tel Binding Protein (MTBP/TRF2), during different stages of spermiogenesis. At all stages of the L929 cell cycle, MTBP/TRF2 is co-localized with Tel. The only Tel order found in this cell type is similar to the Rabl-orientation, probably
AB - Observations of the organization and distribution of telomeres (Tel) in somatic tissues still remain controversial. The Tel topography revealed by modern microscopy shows them to be associated with the nuclear envelope (NE) in a wide variety of eukaryotic cells, although not at the Rabl orientation (peripheral position at one pole of the nucleus at prophase). We used two cell types that have different nuclear architectures. The cell line L929 shows lack of any rigid Tel architecture in the nucleus. In contrast, spermatozoa have a precise architecture established during spermiogenesis. We observed Tel and membrane Tel binding protein (MTBP/TRF2) position by immunoFISH in L929 cells and by immunofluorescence and immunogold electron microscopy, using antibodies against Membrane Tel Binding Protein (MTBP/TRF2), during different stages of spermiogenesis. At all stages of the L929 cell cycle, MTBP/TRF2 is co-localized with Tel. The only Tel order found in this cell type is similar to the Rabl-orientation, probably
U2 - 10.1089/109454503769684784.
DO - 10.1089/109454503769684784.
M3 - статья
VL - 6
SP - 107
EP - 121
JO - Rejuvenation Research
JF - Rejuvenation Research
SN - 1549-1684
IS - 2
ER -
ID: 5444651