Research output: Contribution to journal › Article › peer-review
Synthesis and Study of the Biologically Active Lysozyme–Silver Nanoparticles–Montmorillonite K10 Complexes. / Golubeva, O. Yu.; Shamova, O. V.; Yakovlev, A. V.; Zharkova, M. S.
In: Glass Physics and Chemistry, Vol. 42, No. 1, 2016, p. 87-94.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Synthesis and Study of the Biologically Active Lysozyme–Silver Nanoparticles–Montmorillonite K10 Complexes
AU - Golubeva, O. Yu.
AU - Shamova, O. V.
AU - Yakovlev, A. V.
AU - Zharkova, M. S.
PY - 2016
Y1 - 2016
N2 - Bioinorganic complexes based on silver nanoparticles coated with lysozyme shell (bioconjugates) and aluminosilicate matrices have been synthesizeed. Layered aluminosilicates with the structure of montmorillonite of grade K10 were used as matrices. Complexes with the silver mass fraction 0.3% (from the chemical analysis data) were obtained through fivefold treatment of the aluminosilicate matrix by a sol of bioconjugates with an average particle size of 18 nm and a thickness of the biological cell of similar to 4 nm. The produced biocomplexes were investigated by the methods of X-ray diffraction, scanning electron microscopy, and UV spectroscopy. The samples' antibacterial activity against Gram-negative (E. coli ML-35p, P. aeruginosa ATCC 27853) and Gram-positive (MRSA ATCC 33591, L. monocytogenes EGD) bacteria has been studied. The presence of the biocomplex activity toward antibiotic-resistant strains E. coli ML-35p and MRSA has been revealed.
AB - Bioinorganic complexes based on silver nanoparticles coated with lysozyme shell (bioconjugates) and aluminosilicate matrices have been synthesizeed. Layered aluminosilicates with the structure of montmorillonite of grade K10 were used as matrices. Complexes with the silver mass fraction 0.3% (from the chemical analysis data) were obtained through fivefold treatment of the aluminosilicate matrix by a sol of bioconjugates with an average particle size of 18 nm and a thickness of the biological cell of similar to 4 nm. The produced biocomplexes were investigated by the methods of X-ray diffraction, scanning electron microscopy, and UV spectroscopy. The samples' antibacterial activity against Gram-negative (E. coli ML-35p, P. aeruginosa ATCC 27853) and Gram-positive (MRSA ATCC 33591, L. monocytogenes EGD) bacteria has been studied. The presence of the biocomplex activity toward antibiotic-resistant strains E. coli ML-35p and MRSA has been revealed.
KW - montmorillonite
KW - K10
KW - nanoparticles
KW - lysozyme
KW - bioconjugates
KW - biocomplexes
KW - antimicrobial activity
KW - antibiotics
KW - antibiotic resistance
KW - NANOCOMPOSITES
KW - ADSORPTION
U2 - 10.1134/S1087659616010041
DO - 10.1134/S1087659616010041
M3 - Article
VL - 42
SP - 87
EP - 94
JO - Glass Physics and Chemistry
JF - Glass Physics and Chemistry
SN - 1087-6596
IS - 1
ER -
ID: 7619518