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Substrate Feeding “DNA-Cassette” Improves the Background of Binary (Split) DNAzyme Sensor. / Kostyuk, Y. S.; Zemerova, T.P.; Rubel, Maria S.; Rubel, Aleksandr A.

In: Cell and Tissue Biology, Vol. 19, No. Suppl. 1, 19.08.2025, p. S65-S70.

Research output: Contribution to journal › Article › peer-review

Harvard

Kostyuk, YS, Zemerova, TP, Rubel, MS & Rubel, AA 2025, 'Substrate Feeding “DNA-Cassette” Improves the Background of Binary (Split) DNAzyme Sensor', Cell and Tissue Biology, vol. 19, no. Suppl. 1, pp. S65-S70. https://doi.org/10.1134/S1990519X25600152

APA

Kostyuk, Y. S., Zemerova, T. P., Rubel, M. S., & Rubel, A. A. (2025). Substrate Feeding “DNA-Cassette” Improves the Background of Binary (Split) DNAzyme Sensor. Cell and Tissue Biology, 19(Suppl. 1), S65-S70. https://doi.org/10.1134/S1990519X25600152

Vancouver

Kostyuk YS, Zemerova TP, Rubel MS, Rubel AA. Substrate Feeding “DNA-Cassette” Improves the Background of Binary (Split) DNAzyme Sensor. Cell and Tissue Biology. 2025 Aug 19;19(Suppl. 1):S65-S70. https://doi.org/10.1134/S1990519X25600152

Author

Kostyuk, Y. S. ; Zemerova, T.P. ; Rubel, Maria S. ; Rubel, Aleksandr A. / Substrate Feeding “DNA-Cassette” Improves the Background of Binary (Split) DNAzyme Sensor. In: Cell and Tissue Biology. 2025 ; Vol. 19, No. Suppl. 1. pp. S65-S70.

BibTeX

@article{363b8bd572c24885a54ac719adbdf069,
title = "Substrate Feeding “DNA-Cassette” Improves the Background of Binary (Split) DNAzyme Sensor",
abstract = "Abstract: Objective: The enhanced performance of molecular sensors for diagnostics by utilizing a DNA-cassette nanostructure can improve the delivery of fluorogenic substrates to the catalytic core of a binary (split) DNAzyme (BiDz), resulting in reduced background noise compared to traditional designs. Methods: In vitro methods of fluorescence measurement and gel shifts to assess secondary structures, sensitivity and selectivity of the designs were used. Results and Discussion: The modified sensor had reduced background than DNA-cassette-free BiDz sensor of traditional design. Conclusions: This innovation aims to increase the sensitivity and reliability of nucleic acid-based sensors in future in vivo diagnostic applications.",
keywords = "DNAzyme, Sensor, DNA-Cassette, DNA-nanomachine, RNA-cleaving DNAzyme (Dz), binary DNAzyme (BiDz), selectivity factor (SF)",
author = "Kostyuk, {Y. S.} and T.P. Zemerova and Rubel, {Maria S.} and Rubel, {Aleksandr A.}",
note = "ostyuk, Y.S., Rubel, M.S., Zemerova, T.P. et al. Substrate Feeding “DNA-Cassette” Improves the Background of Binary (Split) DNAzyme Sensor. Cell Tiss. Biol. 19 (Suppl 1), S65–S70 (2025). https://doi.org/10.1134/S1990519X25600152",
year = "2025",
month = aug,
day = "19",
doi = "10.1134/S1990519X25600152",
language = "English",
volume = "19",
pages = "S65--S70",
journal = "Cell and Tissue Biology",
issn = "1990-519X",
publisher = "МАИК {"}Наука/Интерпериодика{"}",
number = "Suppl. 1",

}

RIS

TY - JOUR

T1 - Substrate Feeding “DNA-Cassette” Improves the Background of Binary (Split) DNAzyme Sensor

AU - Kostyuk, Y. S.

AU - Zemerova, T.P.

AU - Rubel, Maria S.

AU - Rubel, Aleksandr A.

N1 - ostyuk, Y.S., Rubel, M.S., Zemerova, T.P. et al. Substrate Feeding “DNA-Cassette” Improves the Background of Binary (Split) DNAzyme Sensor. Cell Tiss. Biol. 19 (Suppl 1), S65–S70 (2025). https://doi.org/10.1134/S1990519X25600152

PY - 2025/8/19

Y1 - 2025/8/19

N2 - Abstract: Objective: The enhanced performance of molecular sensors for diagnostics by utilizing a DNA-cassette nanostructure can improve the delivery of fluorogenic substrates to the catalytic core of a binary (split) DNAzyme (BiDz), resulting in reduced background noise compared to traditional designs. Methods: In vitro methods of fluorescence measurement and gel shifts to assess secondary structures, sensitivity and selectivity of the designs were used. Results and Discussion: The modified sensor had reduced background than DNA-cassette-free BiDz sensor of traditional design. Conclusions: This innovation aims to increase the sensitivity and reliability of nucleic acid-based sensors in future in vivo diagnostic applications.

AB - Abstract: Objective: The enhanced performance of molecular sensors for diagnostics by utilizing a DNA-cassette nanostructure can improve the delivery of fluorogenic substrates to the catalytic core of a binary (split) DNAzyme (BiDz), resulting in reduced background noise compared to traditional designs. Methods: In vitro methods of fluorescence measurement and gel shifts to assess secondary structures, sensitivity and selectivity of the designs were used. Results and Discussion: The modified sensor had reduced background than DNA-cassette-free BiDz sensor of traditional design. Conclusions: This innovation aims to increase the sensitivity and reliability of nucleic acid-based sensors in future in vivo diagnostic applications.

KW - DNAzyme

KW - Sensor

KW - DNA-Cassette

KW - DNA-nanomachine

KW - RNA-cleaving DNAzyme (Dz)

KW - binary DNAzyme (BiDz)

KW - selectivity factor (SF)

UR - https://www.mendeley.com/catalogue/7eaac28d-3926-32b7-a1b6-3731c2d59a09/

U2 - 10.1134/S1990519X25600152

DO - 10.1134/S1990519X25600152

M3 - Article

VL - 19

SP - S65-S70

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - Suppl. 1

ER -

ID: 140234198