TY - JOUR

T1 - Sub–1 min separation in sequential injection chromatography for determination of synthetic water-soluble dyes in pharmaceutical formulation

AU - Davletbaeva, Polina

AU - Chocholouš, Petr

AU - Bulatov, Andrey

AU - Šatínský, Dalibor

AU - Solich, Petr

N1 - Publisher Copyright: © 2017

PY - 2017/9/5

Y1 - 2017/9/5

N2 - Sequential Injection Chromatography (SIC) evolved from fast and automated non-separation Sequential Injection Analysis (SIA) into chromatographic separation method for multi-element analysis. However, the speed of the measurement (sample throughput) is due to chromatography significantly reduced. In this paper, a sub–1 min separation using medium polar cyano monolithic column (5 mm × 4.6 mm) resulted in fast and green separation with sample throughput comparable with non-separation flow methods The separation of three synthetic water-soluble dyes (sunset yellow FCF, carmoisine and green S) was in a gradient elution mode (0.02% ammonium acetate, pH 6.7 – water) with flow rate of 3.0 mL min−1 corresponding with sample throughput of 30 h−1. Spectrophotometric detection wavelengths were set to 480, 516 and 630 nm and 10 Hz data collection rate. The performance of the separation was described and discussed (peak capacities 3.48–7.67, peak symmetries 1.72–1.84 and resolutions 1.42–1.88). The method was represented by validation parameters: LODs of 0.15-0.35 mg L−1, LOQs of 0.50–1.25 mg L−1, calibration ranges 0.50-150.00 mg L−1 (r > 0.998) and repeatability at 10.0 mg L−1 of RSD ≤ 0.98% (n = 6). The method was used for determination of the dyes in “forest berries” colored pharmaceutical cough-cold formulation. The sample matrix – pharmaceuticals and excipients were not interfering with vis determination because of no retention in the separation column and colorless nature. The results proved the concept of fast and green chromatography approach using very short medium polar monolithic column in SIC.

AB - Sequential Injection Chromatography (SIC) evolved from fast and automated non-separation Sequential Injection Analysis (SIA) into chromatographic separation method for multi-element analysis. However, the speed of the measurement (sample throughput) is due to chromatography significantly reduced. In this paper, a sub–1 min separation using medium polar cyano monolithic column (5 mm × 4.6 mm) resulted in fast and green separation with sample throughput comparable with non-separation flow methods The separation of three synthetic water-soluble dyes (sunset yellow FCF, carmoisine and green S) was in a gradient elution mode (0.02% ammonium acetate, pH 6.7 – water) with flow rate of 3.0 mL min−1 corresponding with sample throughput of 30 h−1. Spectrophotometric detection wavelengths were set to 480, 516 and 630 nm and 10 Hz data collection rate. The performance of the separation was described and discussed (peak capacities 3.48–7.67, peak symmetries 1.72–1.84 and resolutions 1.42–1.88). The method was represented by validation parameters: LODs of 0.15-0.35 mg L−1, LOQs of 0.50–1.25 mg L−1, calibration ranges 0.50-150.00 mg L−1 (r > 0.998) and repeatability at 10.0 mg L−1 of RSD ≤ 0.98% (n = 6). The method was used for determination of the dyes in “forest berries” colored pharmaceutical cough-cold formulation. The sample matrix – pharmaceuticals and excipients were not interfering with vis determination because of no retention in the separation column and colorless nature. The results proved the concept of fast and green chromatography approach using very short medium polar monolithic column in SIC.

KW - Cyano monolithic column

KW - Fast separation

KW - Green chromatography

KW - No-solvent mobile phase

KW - Sequential injection chromatography

KW - Water-soluble food dyes

UR - http://www.scopus.com/inward/record.url?scp=85019984072&partnerID=8YFLogxK

U2 - 10.1016/j.jpba.2017.05.036

DO - 10.1016/j.jpba.2017.05.036

M3 - Article

C2 - 28586724

AN - SCOPUS:85019984072

VL - 143

SP - 123

EP - 129

JO - Journal of Pharmaceutical and Biomedical Analysis

JF - Journal of Pharmaceutical and Biomedical Analysis

SN - 0731-7085

ER -