Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review
Studying Porifera WBR Using the Calcerous Sponges Leucosolenia. / Лавров, Андрей Игоревич; Ересковский, Александр Вадимович.
Whole-body regeneration. Methods in Molecular Biology. ed. / Simon Blanchoud; Brigitte Galliot. Springer Nature, 2022. p. 69-93 (Methods in Molecular Biology; Vol. 2450).Research output: Chapter in Book/Report/Conference proceeding › Chapter › Research › peer-review
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TY - CHAP
T1 - Studying Porifera WBR Using the Calcerous Sponges Leucosolenia
AU - Лавров, Андрей Игоревич
AU - Ересковский, Александр Вадимович
N1 - Publisher Copyright: © 2022, The Author(s).
PY - 2022
Y1 - 2022
N2 - Sponges (Porifera), basal nonbilaterian metazoans, are well known for their high regenerative capacities ranging from reparation of a lost body wall to whole-body regeneration from a small piece of tissues or even from dissociated cells. Sponges from different clades utilize different cell sources and various morphological processes to complete the regeneration. This variety makes these animals promising models for studying the evolution of regeneration in Metazoa. However, there are few publications concerning the regenerative mechanisms in sponges. This could be partially explained by the delicacy of sponge tissues, which requires modifying and fine adjusting of common research protocols. The current chapter describes various methods for studying regeneration processes in the marine calcareous sponge, Leucosolenia. Provided protocols span all significant research steps: from sponge collection and surgical operations to various types of microscopy and immunohistochemical studies.
AB - Sponges (Porifera), basal nonbilaterian metazoans, are well known for their high regenerative capacities ranging from reparation of a lost body wall to whole-body regeneration from a small piece of tissues or even from dissociated cells. Sponges from different clades utilize different cell sources and various morphological processes to complete the regeneration. This variety makes these animals promising models for studying the evolution of regeneration in Metazoa. However, there are few publications concerning the regenerative mechanisms in sponges. This could be partially explained by the delicacy of sponge tissues, which requires modifying and fine adjusting of common research protocols. The current chapter describes various methods for studying regeneration processes in the marine calcareous sponge, Leucosolenia. Provided protocols span all significant research steps: from sponge collection and surgical operations to various types of microscopy and immunohistochemical studies.
KW - Apoptosis
KW - Microscopy
KW - Proliferation
KW - Protocols
KW - Regeneration
KW - Sponges
KW - Surgical operations
KW - Ultrastructure
KW - Animals
KW - Porifera
UR - http://www.scopus.com/inward/record.url?scp=85127389296&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/193ee72a-11a2-3928-b9dd-7fd565942072/
U2 - 10.1007/978-1-0716-2172-1_4
DO - 10.1007/978-1-0716-2172-1_4
M3 - Chapter
C2 - 35359303
T3 - Methods in Molecular Biology
SP - 69
EP - 93
BT - Whole-body regeneration. Methods in Molecular Biology
A2 - Blanchoud, Simon
A2 - Galliot, Brigitte
PB - Springer Nature
ER -
ID: 93882935