TY - JOUR

T1 - Study of the variability of pea response to transformation with Agrobacterium tumefaciens and Agrobacterium rhizogenes strains

AU - Lutova, L. A.

AU - Sharova, N. V.

PY - 1993

Y1 - 1993

N2 - The work is devoted to studying in the pea interspecies variability of capacity to be transformed by A. tumefaciens and A. rhizogenes strains. Transformation experiments were accomplished on inbred lines from genetic collection of All-Russian Research Institute for Agricultural Microbiology, as well as on unable to nodulation mutant lines (Nod- sym 5 and sym 8) and their parental Sparkle variety. Axenic seedlings, stem and leaf explants of different genetic forms were inoculated with bacterial suspension; tumor- and root formation in inoculation sites were assessed visually 40 days after inoculation by comparison with control plants and explants. The variability of pea susceptibility to transformation was observed. The line 32/11 with highest ability for both transformation and regeneration was revealed; the possible connection between these processes is discussed. The genetic forms with untypical genetic response to inoculation with Agrobacterium strains were revealed: for example, those forming roots instead of undifferentiated tumors when transformed with A. tumefaciens wildtype strains or, vice versa, forming tumors when transformed with A. rhizogenes. The variability of phenotypic characters of pea tissues transformed with certain Agrobacterium strains reflects the variability of pea hormone balances and hormone susceptibilities. The direct connection between nodulation and transformation capacities was not revealed, but the phenotypic effects of mutants Nod- and the parental variety transformation makes it possible to discuss the peculiarities of hormone status of these forms. The strain-specificity of transformation and its dependence on plant genotype-bacterial strain combination was demonstrated.

AB - The work is devoted to studying in the pea interspecies variability of capacity to be transformed by A. tumefaciens and A. rhizogenes strains. Transformation experiments were accomplished on inbred lines from genetic collection of All-Russian Research Institute for Agricultural Microbiology, as well as on unable to nodulation mutant lines (Nod- sym 5 and sym 8) and their parental Sparkle variety. Axenic seedlings, stem and leaf explants of different genetic forms were inoculated with bacterial suspension; tumor- and root formation in inoculation sites were assessed visually 40 days after inoculation by comparison with control plants and explants. The variability of pea susceptibility to transformation was observed. The line 32/11 with highest ability for both transformation and regeneration was revealed; the possible connection between these processes is discussed. The genetic forms with untypical genetic response to inoculation with Agrobacterium strains were revealed: for example, those forming roots instead of undifferentiated tumors when transformed with A. tumefaciens wildtype strains or, vice versa, forming tumors when transformed with A. rhizogenes. The variability of phenotypic characters of pea tissues transformed with certain Agrobacterium strains reflects the variability of pea hormone balances and hormone susceptibilities. The direct connection between nodulation and transformation capacities was not revealed, but the phenotypic effects of mutants Nod- and the parental variety transformation makes it possible to discuss the peculiarities of hormone status of these forms. The strain-specificity of transformation and its dependence on plant genotype-bacterial strain combination was demonstrated.

UR - http://www.scopus.com/inward/record.url?scp=0027762521&partnerID=8YFLogxK

M3 - Article

AN - SCOPUS:0027762521

VL - 29

SP - 1656

EP - 1672

JO - ГЕНЕТИКА

JF - ГЕНЕТИКА

SN - 0016-6758

IS - 10

ER -