TY - JOUR

T1 - Study of a new strain of Sanchytrium tribonematis expands our knowledge on Sanchytriomycota (Fungi)

AU - Вишняков, Андрей Экскустадианович

AU - Селюк, Алексей Олегович

AU - Цветкова, Виктория Сергеевна

AU - Карпов, Сергей Алексеевич

PY - 2023/10/1

Y1 - 2023/10/1

N2 - Recently, phylogenomic analyses of two parasitic fungi with amoeboid zoospores and long kinetosomes, the sanchytrids Amoeboradix gromovi and Sanchytrium tribonematis, showed that they formed a clade Sanchytriomycota sister to Blastocladiomycota. Sanchytrid species diversity is still very low and most of the isolates from different places belong to S. tribonematis. Here, we present a new strain X-137 CCPP ZIN RAS of fresh-water S. tribonematis having a peculiar morphology and an unusual stage in the life cycle. Its zoospores have a kinetosome composed of 9 microtubular singlets along its whole length (1.2 μm) with the pseudocilium containing an axoneme of 4 microtubules and enable to form an extremely long posterior filopodium (up to 10 body lengths). The kinetosome develops from a centriole of the centrosomal apparatus in sporangium, while another centriole becomes a non-flagellar kinetosome. Big zoospores with several pseudocilia and many (up to eleven) kinetosomes per cell have been found in S. tribonematis culture. They are probably a result of several zoospore or gamete fusions. These findings expand our still poor knowledge on the cell structure and biology of Sanchytriomycota.

AB - Recently, phylogenomic analyses of two parasitic fungi with amoeboid zoospores and long kinetosomes, the sanchytrids Amoeboradix gromovi and Sanchytrium tribonematis, showed that they formed a clade Sanchytriomycota sister to Blastocladiomycota. Sanchytrid species diversity is still very low and most of the isolates from different places belong to S. tribonematis. Here, we present a new strain X-137 CCPP ZIN RAS of fresh-water S. tribonematis having a peculiar morphology and an unusual stage in the life cycle. Its zoospores have a kinetosome composed of 9 microtubular singlets along its whole length (1.2 μm) with the pseudocilium containing an axoneme of 4 microtubules and enable to form an extremely long posterior filopodium (up to 10 body lengths). The kinetosome develops from a centriole of the centrosomal apparatus in sporangium, while another centriole becomes a non-flagellar kinetosome. Big zoospores with several pseudocilia and many (up to eleven) kinetosomes per cell have been found in S. tribonematis culture. They are probably a result of several zoospore or gamete fusions. These findings expand our still poor knowledge on the cell structure and biology of Sanchytriomycota.

KW - Centrioles

KW - Kinetid structure

KW - Life cycle

KW - Pseudocilium

KW - Sanchytrids

UR - https://www.mendeley.com/catalogue/ba914daa-722f-3f43-be34-bb1d2e536ebe/

U2 - 10.1007/s11557-023-01917-1

DO - 10.1007/s11557-023-01917-1

M3 - Article

VL - 22

JO - Mycological Progress

JF - Mycological Progress

SN - 1617-416X

IS - 10

M1 - 69

ER -