Research output: Contribution to journal › Article › peer-review
Structure of DNA complexes with chromosomal protein HMGB1 and histone H1 in the presence of manganese ions: 2. Vibrational circular dichroism spectroscopy. / Polyanichko, A.M.; Vorob'ev, V.I.; Chikhirzhina, E.V.
In: Molecular Biology, Vol. 47, No. 2, 2013, p. 299-306.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Structure of DNA complexes with chromosomal protein HMGB1 and histone H1 in the presence of manganese ions: 2. Vibrational circular dichroism spectroscopy
AU - Polyanichko, A.M.
AU - Vorob'ev, V.I.
AU - Chikhirzhina, E.V.
PY - 2013
Y1 - 2013
N2 - DNA complexes with nonhistone HMGB1 chromatin protein and histone H1 in the presence of manganese ions were studied using methods of absorption and circular dichroism spectroscopy in the infrared region. It was demonstrated that the method provides good results, even for solutions that contain large particles, which cause scattering in UV region. It was determined that manganese ions in the complex are able to coordinate not only to different chemical groups in DNA, but also to dicarboxylic acid residues of the HMGB1 protein, which stimulates DNA condensation and slightly weakens DNA-protein interactions in the complex. © 2013 Pleiades Publishing, Ltd.
AB - DNA complexes with nonhistone HMGB1 chromatin protein and histone H1 in the presence of manganese ions were studied using methods of absorption and circular dichroism spectroscopy in the infrared region. It was demonstrated that the method provides good results, even for solutions that contain large particles, which cause scattering in UV region. It was determined that manganese ions in the complex are able to coordinate not only to different chemical groups in DNA, but also to dicarboxylic acid residues of the HMGB1 protein, which stimulates DNA condensation and slightly weakens DNA-protein interactions in the complex. © 2013 Pleiades Publishing, Ltd.
U2 - 10.1134/S0026893313020118
DO - 10.1134/S0026893313020118
M3 - Article
VL - 47
SP - 299
EP - 306
JO - Molecular Biology
JF - Molecular Biology
SN - 0026-8933
IS - 2
ER -
ID: 5700745