TY - JOUR

T1 - Stoichiometry and affinity of thioflavin T binding to Sup35p amyloid fibrils

AU - Sulatskaya, Anna I.

AU - Kuznetsova, Irina M.

AU - Belousov, Mikhail V.

AU - Bondarev, Stanislav A.

AU - Zhouravleva, Galina A.

AU - Turoverov, Konstantin K.

PY - 2016

Y1 - 2016

N2 - In this work two modes of binding of the fluorescent probe thioflavin T to yeast prion protein Sup35p amyloid fibrils were revealed by absorption spectrometry of solutions prepared by equilibrium microdialysis. These binding modes exhibited significant differences in binding affinity and stoichiometry. Moreover, the absorption spectrum and the molar extinction coefficient of the dye bound in each mode were determined. The fluorescence quantum yield of the dye bound in each mode was determined via a spectrofluorimetric study of the same solutions in which the recorded fluorescence intensity was corrected for the primary inner filter effect. As previously predicted, the existence of one of the detected binding modes may be due to the incorporation of the dye into the grooves along the fiber axis perpendicular to the β-sheets of the fibrils. It was assumed that the second type of binding with higher affinity may be due to the existence of ThT binding sites that are localized to areas where amyloid fibrils are cl

AB - In this work two modes of binding of the fluorescent probe thioflavin T to yeast prion protein Sup35p amyloid fibrils were revealed by absorption spectrometry of solutions prepared by equilibrium microdialysis. These binding modes exhibited significant differences in binding affinity and stoichiometry. Moreover, the absorption spectrum and the molar extinction coefficient of the dye bound in each mode were determined. The fluorescence quantum yield of the dye bound in each mode was determined via a spectrofluorimetric study of the same solutions in which the recorded fluorescence intensity was corrected for the primary inner filter effect. As previously predicted, the existence of one of the detected binding modes may be due to the incorporation of the dye into the grooves along the fiber axis perpendicular to the β-sheets of the fibrils. It was assumed that the second type of binding with higher affinity may be due to the existence of ThT binding sites that are localized to areas where amyloid fibrils are cl

U2 - 10.1371/journal.pone.0156314

DO - 10.1371/journal.pone.0156314

M3 - Article

VL - 11

SP - e0156314 (1-14)

JO - PLoS ONE

JF - PLoS ONE

SN - 1932-6203

IS - 5

ER -