Stabilization of Double-Stranded Poly(A)·Poly(U) with ZnTMPyP4 Metalloporphyrin in Aqueous Solution

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Stabilization of Double-Stranded Poly(A)·Poly(U) with ZnTMPyP4 Metalloporphyrin in Aqueous Solution. / Kudrev, A. G.

In: Russian Journal of General Chemistry, Vol. 90, No. 12, 12.2020, p. 2281-2288.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{123b49ed9bc24394b1795c21a69766e8,

title = "Stabilization of Double-Stranded Poly(A)·Poly(U) with ZnTMPyP4 Metalloporphyrin in Aqueous Solution",

abstract = "Abstract: UV-Vis absorption spectra of aqueous solutions containing both metalloporphyrin Zn(X)TMPyP4 [H2TMPyP4—5,10,15,20-tetrakis(1-methylpyridin-4-yl)-21H,23H-porphyrin] and synthetic polyadenylic-polyuridylic acid Poly(A)·Poly(U) over the temperature range from 20 to 70°C (pH = 7.0, I = 0.15 M.) have been analyzed. Deconvolution of the spectrometric data matrix, without postulating a physicochemical equilibrium model, has allowed estimation of the contribution of the Poly(A)·Poly(U)·(ZnTMPyP4)n complex to the total change in the spectra. Chemometric analysis has shown an increase in the melting temperature of this ternary complex by 9.4°С compared to pure polyribonucleotide, which indicates the stabilization of the bonds between nucleic bases in the Poly(A)·Poly(U) polynucleotide under the influence of bound porphyrin.",

keywords = "biopolymer stabilization, chemometric analysis, Poly(A)·Poly(U), spectrophotometry, ZnTMPyP4",

author = "Kudrev, {A. G.}",

note = "Publisher Copyright: {\textcopyright} 2020, Pleiades Publishing, Ltd.",

year = "2020",

month = dec,

doi = "10.1134/S1070363220120105",

language = "English",

volume = "90",

pages = "2281--2288",

journal = "Russian Journal of General Chemistry",

issn = "1070-3632",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "12",

}

RIS

TY - JOUR

T1 - Stabilization of Double-Stranded Poly(A)·Poly(U) with ZnTMPyP4 Metalloporphyrin in Aqueous Solution

AU - Kudrev, A. G.

PY - 2020/12

Y1 - 2020/12

N2 - Abstract: UV-Vis absorption spectra of aqueous solutions containing both metalloporphyrin Zn(X)TMPyP4 [H2TMPyP4—5,10,15,20-tetrakis(1-methylpyridin-4-yl)-21H,23H-porphyrin] and synthetic polyadenylic-polyuridylic acid Poly(A)·Poly(U) over the temperature range from 20 to 70°C (pH = 7.0, I = 0.15 M.) have been analyzed. Deconvolution of the spectrometric data matrix, without postulating a physicochemical equilibrium model, has allowed estimation of the contribution of the Poly(A)·Poly(U)·(ZnTMPyP4)n complex to the total change in the spectra. Chemometric analysis has shown an increase in the melting temperature of this ternary complex by 9.4°С compared to pure polyribonucleotide, which indicates the stabilization of the bonds between nucleic bases in the Poly(A)·Poly(U) polynucleotide under the influence of bound porphyrin.

AB - Abstract: UV-Vis absorption spectra of aqueous solutions containing both metalloporphyrin Zn(X)TMPyP4 [H2TMPyP4—5,10,15,20-tetrakis(1-methylpyridin-4-yl)-21H,23H-porphyrin] and synthetic polyadenylic-polyuridylic acid Poly(A)·Poly(U) over the temperature range from 20 to 70°C (pH = 7.0, I = 0.15 M.) have been analyzed. Deconvolution of the spectrometric data matrix, without postulating a physicochemical equilibrium model, has allowed estimation of the contribution of the Poly(A)·Poly(U)·(ZnTMPyP4)n complex to the total change in the spectra. Chemometric analysis has shown an increase in the melting temperature of this ternary complex by 9.4°С compared to pure polyribonucleotide, which indicates the stabilization of the bonds between nucleic bases in the Poly(A)·Poly(U) polynucleotide under the influence of bound porphyrin.

KW - biopolymer stabilization

KW - chemometric analysis

KW - Poly(A)·Poly(U)

KW - spectrophotometry

KW - ZnTMPyP4

UR - http://www.scopus.com/inward/record.url?scp=85100584442&partnerID=8YFLogxK

U2 - 10.1134/S1070363220120105

DO - 10.1134/S1070363220120105

M3 - Article

AN - SCOPUS:85100584442

VL - 90

SP - 2281

EP - 2288

JO - Russian Journal of General Chemistry

JF - Russian Journal of General Chemistry

SN - 1070-3632

IS - 12

ER -

ID: 88872040