Research output: Contribution to journal › Article › peer-review
Spread Layers of Lysozyme Microgel at Liquid Surface. / Milyaeva, Olga Yu; Akentiev, Alexander V.; Bykov, Alexey G.; Lin, Shi Yow; Loglio, Giuseppe; Miller, Reinhard; Michailov, Alexander V.; Rotanova, Ksenia Yu; Noskov, Boris A.
In: Polymers, Vol. 14, No. 19, 3979, 23.09.2022.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Spread Layers of Lysozyme Microgel at Liquid Surface
AU - Milyaeva, Olga Yu
AU - Akentiev, Alexander V.
AU - Bykov, Alexey G.
AU - Lin, Shi Yow
AU - Loglio, Giuseppe
AU - Miller, Reinhard
AU - Michailov, Alexander V.
AU - Rotanova, Ksenia Yu
AU - Noskov, Boris A.
N1 - Milyaeva, O.Y.; Akentiev, A.V.; Bykov, A.G.; Lin, S.-Y.; Loglio, G.; Miller, R.; Michailov, A.V.; Rotanova, K.Y.; Noskov, B.A. Spread Layers of Lysozyme Microgel at Liquid Surface. Polymers 2022, 14, 3979. https://doi.org/10.3390/polym14193979
PY - 2022/9/23
Y1 - 2022/9/23
N2 - The spread layers of lysozyme (LYS) microgel particles were studied by surface dilational rheology, infrared reflection–absorption spectra, Brewster angle microscopy, atomic force microscopy, and scanning electron microscopy. It is shown that the properties of LYS microgel layers differ significantly from those of ß-lactoglobulin (BLG) microgel layers. In the latter case, the spread protein layer is mainly a monolayer, and the interactions between particles lead to the increase in the dynamic surface elasticity by up to 140 mN/m. In contrast, the dynamic elasticity of the LYS microgel layer does not exceed the values for pure protein layers. The compression isotherms also do not exhibit specific features of the layer collapse that are characteristic for the layers of BLG aggregates. LYS aggregates form trough three-dimensional clusters directly during the spreading process, and protein spherulites do not spread further along the interface. As a result, the liquid surface contains large, almost empty regions and some patches of high local concentration of the microgel particles.
AB - The spread layers of lysozyme (LYS) microgel particles were studied by surface dilational rheology, infrared reflection–absorption spectra, Brewster angle microscopy, atomic force microscopy, and scanning electron microscopy. It is shown that the properties of LYS microgel layers differ significantly from those of ß-lactoglobulin (BLG) microgel layers. In the latter case, the spread protein layer is mainly a monolayer, and the interactions between particles lead to the increase in the dynamic surface elasticity by up to 140 mN/m. In contrast, the dynamic elasticity of the LYS microgel layer does not exceed the values for pure protein layers. The compression isotherms also do not exhibit specific features of the layer collapse that are characteristic for the layers of BLG aggregates. LYS aggregates form trough three-dimensional clusters directly during the spreading process, and protein spherulites do not spread further along the interface. As a result, the liquid surface contains large, almost empty regions and some patches of high local concentration of the microgel particles.
KW - AFM
KW - BAM
KW - IRRAS
KW - lysozyme
KW - microgel particles
KW - SEM
KW - spread layers
KW - surface dilational viscoelasticity
KW - β-lactoglobulin
UR - http://www.scopus.com/inward/record.url?scp=85139876198&partnerID=8YFLogxK
UR - https://www.mendeley.com/catalogue/3f431178-2ed6-35ec-af8b-52a729ec380f/
U2 - 10.3390/polym14193979
DO - 10.3390/polym14193979
M3 - Article
C2 - 36235927
AN - SCOPUS:85139876198
VL - 14
JO - Polymers
JF - Polymers
SN - 2073-4360
IS - 19
M1 - 3979
ER -
ID: 99694916