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Spectrophotometric Evaluation of L-Lysine Concentrations in Water–Organic Solutions. / Nashchekina, Yu A.; Kurdyukova, K. E.; Zorin, I. M.; Mikhailova, N. A.; Bilibin, A. Yu.

In: Technical Physics, Vol. 63, No. 9, 01.09.2018, p. 1341-1344.

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Nashchekina, YA, Kurdyukova, KE, Zorin, IM, Mikhailova, NA & Bilibin, AY 2018, 'Spectrophotometric Evaluation of L-Lysine Concentrations in Water–Organic Solutions', Technical Physics, vol. 63, no. 9, pp. 1341-1344. https://doi.org/10.1134/S106378421809013X

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Nashchekina, Yu A. ; Kurdyukova, K. E. ; Zorin, I. M. ; Mikhailova, N. A. ; Bilibin, A. Yu. / Spectrophotometric Evaluation of L-Lysine Concentrations in Water–Organic Solutions. In: Technical Physics. 2018 ; Vol. 63, No. 9. pp. 1341-1344.

BibTeX

@article{e61eb6980012416fae8728c664236c88,
title = "Spectrophotometric Evaluation of L-Lysine Concentrations in Water–Organic Solutions",
abstract = "Abstract: In this work, the conditions of spectrophotometric evaluation of the ninhydrin assay for L-lysine in a water dimethyl sulfoxide solution were investigated. The absorption spectrum of the L-lysine–ninhydrin reaction product exhibited two maxima at 420 and 500 nm. It was shown that pH of the reaction medium affected the intensity of the product color. The stability of the ninhydrin reaction products was the highest at pH 8–9. The phase proportion of the water–organic solution had little effect on the optical density of the product. The absorption spectra of the ninhydrin reaction products of L-lysine derivatives: Nα-boc-lysine and Nε-z-lysine, indicated that it was the α amino group of lysine that participated in the reaction.",
author = "Nashchekina, {Yu A.} and Kurdyukova, {K. E.} and Zorin, {I. M.} and Mikhailova, {N. A.} and Bilibin, {A. Yu}",
year = "2018",
month = sep,
day = "1",
doi = "10.1134/S106378421809013X",
language = "English",
volume = "63",
pages = "1341--1344",
journal = "Technical Physics",
issn = "1063-7842",
publisher = "Pleiades Publishing",
number = "9",

}

RIS

TY - JOUR

T1 - Spectrophotometric Evaluation of L-Lysine Concentrations in Water–Organic Solutions

AU - Nashchekina, Yu A.

AU - Kurdyukova, K. E.

AU - Zorin, I. M.

AU - Mikhailova, N. A.

AU - Bilibin, A. Yu

PY - 2018/9/1

Y1 - 2018/9/1

N2 - Abstract: In this work, the conditions of spectrophotometric evaluation of the ninhydrin assay for L-lysine in a water dimethyl sulfoxide solution were investigated. The absorption spectrum of the L-lysine–ninhydrin reaction product exhibited two maxima at 420 and 500 nm. It was shown that pH of the reaction medium affected the intensity of the product color. The stability of the ninhydrin reaction products was the highest at pH 8–9. The phase proportion of the water–organic solution had little effect on the optical density of the product. The absorption spectra of the ninhydrin reaction products of L-lysine derivatives: Nα-boc-lysine and Nε-z-lysine, indicated that it was the α amino group of lysine that participated in the reaction.

AB - Abstract: In this work, the conditions of spectrophotometric evaluation of the ninhydrin assay for L-lysine in a water dimethyl sulfoxide solution were investigated. The absorption spectrum of the L-lysine–ninhydrin reaction product exhibited two maxima at 420 and 500 nm. It was shown that pH of the reaction medium affected the intensity of the product color. The stability of the ninhydrin reaction products was the highest at pH 8–9. The phase proportion of the water–organic solution had little effect on the optical density of the product. The absorption spectra of the ninhydrin reaction products of L-lysine derivatives: Nα-boc-lysine and Nε-z-lysine, indicated that it was the α amino group of lysine that participated in the reaction.

UR - http://www.scopus.com/inward/record.url?scp=85053902472&partnerID=8YFLogxK

UR - http://link.springer.com/10.1134/S106378421809013X

UR - http://www.mendeley.com/research/spectrophotometric-evaluation-llysine-concentrations-waterorganic-solutions

U2 - 10.1134/S106378421809013X

DO - 10.1134/S106378421809013X

M3 - Article

AN - SCOPUS:85053902472

VL - 63

SP - 1341

EP - 1344

JO - Technical Physics

JF - Technical Physics

SN - 1063-7842

IS - 9

ER -

ID: 36559663