Research output: Contribution to journal › Article › peer-review
Spectrophotometric Evaluation of L-Lysine Concentrations in Water–Organic Solutions. / Nashchekina, Yu A.; Kurdyukova, K. E.; Zorin, I. M.; Mikhailova, N. A.; Bilibin, A. Yu.
In: Technical Physics, Vol. 63, No. 9, 01.09.2018, p. 1341-1344.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Spectrophotometric Evaluation of L-Lysine Concentrations in Water–Organic Solutions
AU - Nashchekina, Yu A.
AU - Kurdyukova, K. E.
AU - Zorin, I. M.
AU - Mikhailova, N. A.
AU - Bilibin, A. Yu
PY - 2018/9/1
Y1 - 2018/9/1
N2 - Abstract: In this work, the conditions of spectrophotometric evaluation of the ninhydrin assay for L-lysine in a water dimethyl sulfoxide solution were investigated. The absorption spectrum of the L-lysine–ninhydrin reaction product exhibited two maxima at 420 and 500 nm. It was shown that pH of the reaction medium affected the intensity of the product color. The stability of the ninhydrin reaction products was the highest at pH 8–9. The phase proportion of the water–organic solution had little effect on the optical density of the product. The absorption spectra of the ninhydrin reaction products of L-lysine derivatives: Nα-boc-lysine and Nε-z-lysine, indicated that it was the α amino group of lysine that participated in the reaction.
AB - Abstract: In this work, the conditions of spectrophotometric evaluation of the ninhydrin assay for L-lysine in a water dimethyl sulfoxide solution were investigated. The absorption spectrum of the L-lysine–ninhydrin reaction product exhibited two maxima at 420 and 500 nm. It was shown that pH of the reaction medium affected the intensity of the product color. The stability of the ninhydrin reaction products was the highest at pH 8–9. The phase proportion of the water–organic solution had little effect on the optical density of the product. The absorption spectra of the ninhydrin reaction products of L-lysine derivatives: Nα-boc-lysine and Nε-z-lysine, indicated that it was the α amino group of lysine that participated in the reaction.
UR - http://www.scopus.com/inward/record.url?scp=85053902472&partnerID=8YFLogxK
UR - http://link.springer.com/10.1134/S106378421809013X
UR - http://www.mendeley.com/research/spectrophotometric-evaluation-llysine-concentrations-waterorganic-solutions
U2 - 10.1134/S106378421809013X
DO - 10.1134/S106378421809013X
M3 - Article
AN - SCOPUS:85053902472
VL - 63
SP - 1341
EP - 1344
JO - Technical Physics
JF - Technical Physics
SN - 1063-7842
IS - 9
ER -
ID: 36559663