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Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage. / Bozhokin, Mikhail S. ; Bozhkova, Svetlana A. ; Rubel, Aleksandr A.; Sopova, Julia V.; Nashchekina, Yulia A. ; Bildyug, Natalya B. ; Khotin, Mikhail G. .

In: Methods and Protocols, Vol. 77, No. 4, 77, 27.10.2021.

Research output: Contribution to journal › Article › peer-review

Harvard

Bozhokin, MS, Bozhkova, SA, Rubel, AA, Sopova, JV, Nashchekina, YA, Bildyug, NB & Khotin, MG 2021, 'Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage', Methods and Protocols, vol. 77, no. 4, 77.

APA

Bozhokin, M. S., Bozhkova, S. A., Rubel, A. A., Sopova, J. V., Nashchekina, Y. A., Bildyug, N. B., & Khotin, M. G. (2021). Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage. Methods and Protocols, 77(4), [77].

Vancouver

Bozhokin MS, Bozhkova SA, Rubel AA, Sopova JV, Nashchekina YA, Bildyug NB et al. Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage. Methods and Protocols. 2021 Oct 27;77(4). 77.

Author

Bozhokin, Mikhail S. ; Bozhkova, Svetlana A. ; Rubel, Aleksandr A. ; Sopova, Julia V. ; Nashchekina, Yulia A. ; Bildyug, Natalya B. ; Khotin, Mikhail G. . / Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage. In: Methods and Protocols. 2021 ; Vol. 77, No. 4.

BibTeX

@article{bbb59053a1464bc7adbf34ed6c72f546,
title = "Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage",
abstract = "Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegradable scaffold with a premixed cell culture into the damaged area of the articular surface. However, current methods for analyzing the effectiveness of such CECs have significant limitations. This study aimed to compare the SEM technique, classical histology, and cryosectioning for the analysis of CECs transplanted to hyaline cartilage.",
keywords = "SEM, histology, cellular engineered constructs, tissue engineering, hyaline cartilage",
author = "Bozhokin, {Mikhail S.} and Bozhkova, {Svetlana A.} and Rubel, {Aleksandr A.} and Sopova, {Julia V.} and Nashchekina, {Yulia A.} and Bildyug, {Natalya B.} and Khotin, {Mikhail G.}",
note = "Bozhokin, M.S.; Bozhkova, S.A.; Rubel, A.A.; Sopova, J.V.; Nashchekina, Y.A.; Bildyug, N.B.; Khotin, M.G. Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage. Methods Protoc. 2021, 4, 77. https://doi.org/10.3390/mps4040077",
year = "2021",
month = oct,
day = "27",
language = "English",
volume = "77",
journal = "Methods and Protocols — Open Access Journal ",
issn = "2409-9279",
publisher = "MDPI AG",
number = "4",

}

RIS

TY - JOUR

T1 - Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage

AU - Bozhokin, Mikhail S.

AU - Bozhkova, Svetlana A.

AU - Rubel, Aleksandr A.

AU - Sopova, Julia V.

AU - Nashchekina, Yulia A.

AU - Bildyug, Natalya B.

AU - Khotin, Mikhail G.

N1 - Bozhokin, M.S.; Bozhkova, S.A.; Rubel, A.A.; Sopova, J.V.; Nashchekina, Y.A.; Bildyug, N.B.; Khotin, M.G. Specificities of Scanning Electron Microscopy and Histological Methods in Assessing Cell-Engineered Construct Effectiveness for the Recovery of Hyaline Cartilage. Methods Protoc. 2021, 4, 77. https://doi.org/10.3390/mps4040077

PY - 2021/10/27

Y1 - 2021/10/27

N2 - Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegradable scaffold with a premixed cell culture into the damaged area of the articular surface. However, current methods for analyzing the effectiveness of such CECs have significant limitations. This study aimed to compare the SEM technique, classical histology, and cryosectioning for the analysis of CECs transplanted to hyaline cartilage.

AB - Damage to the hyaline layer of the articular surface is an urgent problem for millions of people around the world. At present, a large number of experimental methods are being developed to address this problem, including the transplantation of a cell-engineered construct (CEC) composed of a biodegradable scaffold with a premixed cell culture into the damaged area of the articular surface. However, current methods for analyzing the effectiveness of such CECs have significant limitations. This study aimed to compare the SEM technique, classical histology, and cryosectioning for the analysis of CECs transplanted to hyaline cartilage.

KW - SEM

KW - histology

KW - cellular engineered constructs

KW - tissue engineering

KW - hyaline cartilage

UR - https://www.mdpi.com/2409-9279/4/4/77

M3 - Article

VL - 77

JO - Methods and Protocols — Open Access Journal

JF - Methods and Protocols — Open Access Journal

SN - 2409-9279

IS - 4

M1 - 77

ER -

ID: 87716813