TY - JOUR

T1 - Specific Detection of Neisseria meningitidis by Dual Priming Isothermal Amplification with Scorpion-Shaped Probes

AU - Шкоденко, Любовь Алексеевна

AU - Kuznetsova, Veronica S.

AU - Рубель, Мария Сергеевна

PY - 2025/10/1

Y1 - 2025/10/1

N2 - Abstract: Objective: The real-time isothermal Scorpio-DAMP assay for the determination of bacterial pathogens, specifically targeting commensal organisms, is important for clinical practice. Methods: The assay for Neisseria meningitidis was characterized using a set of lower respiratory tract pathogens, with sensitivity assessed at a genomic equivalent (GE) input of 100 000 for a positive reaction, and had no cross-reactivity. Results: The Scorpio-DAMP assay demonstrated a reaction time less than 30 min for positive slope recognition, with negative percent accuracy and positive percent accuracy of 91.8 and 70.6%, respectively. Discussion: Compared to the conventional PCR, which requires temperature cycling and longer processing times, the Scorpio-DAMP assay offers a more accessible solution for high-throughput and low-resource testing environments. Conclusions: The Scorpio-DAMP assay represents a promising alternative for rapid bacterial pathogen detection, particularly suitable for settings with limited resources.

AB - Abstract: Objective: The real-time isothermal Scorpio-DAMP assay for the determination of bacterial pathogens, specifically targeting commensal organisms, is important for clinical practice. Methods: The assay for Neisseria meningitidis was characterized using a set of lower respiratory tract pathogens, with sensitivity assessed at a genomic equivalent (GE) input of 100 000 for a positive reaction, and had no cross-reactivity. Results: The Scorpio-DAMP assay demonstrated a reaction time less than 30 min for positive slope recognition, with negative percent accuracy and positive percent accuracy of 91.8 and 70.6%, respectively. Discussion: Compared to the conventional PCR, which requires temperature cycling and longer processing times, the Scorpio-DAMP assay offers a more accessible solution for high-throughput and low-resource testing environments. Conclusions: The Scorpio-DAMP assay represents a promising alternative for rapid bacterial pathogen detection, particularly suitable for settings with limited resources.

KW - DAMP

KW - Neisseria meningitidis

KW - Scorpio probe

KW - lower respiratory system

UR - https://www.mendeley.com/catalogue/1e636d10-9e20-379c-8f4b-fa6b27c54232/

U2 - 10.1134/S1990519X25600413

DO - 10.1134/S1990519X25600413

M3 - Article

VL - 19

SP - 481

EP - 486

JO - Cell and Tissue Biology

JF - Cell and Tissue Biology

SN - 1990-519X

IS - 5

ER -