TY - JOUR

T1 - Species-specific proteins in the oviducts of snail sibling species

T2 - Proteotranscriptomic study of littorina fabalis and l. obtusata

AU - Lobov, Arseniy A.

AU - Babkina, Irina Y.

AU - Danilov, Lavrentii G.

AU - Masharskiy, Alexey E.

AU - Predeus, Alexander V.

AU - Mikhailova, Natalia A.

AU - Granovitch, Andrei I.

AU - Maltseva, Arina L.

N1 - Lobov, A.A.; Babkina, I.Y.; Danilov, L.G.; Masharskiy, A.E.; Predeus, A.V.; Mikhailova, N.A.; Granovitch, A.I.; Maltseva, A.L. Species-Specific Proteins in the Oviducts of Snail Sibling Species: Proteotranscriptomic Study of Littorina fabalis and L. obtusata. Biology 2021, 10, 1087. https://doi.org/10.3390/biology10111087

PY - 2021/11

Y1 - 2021/11

N2 - Genus Littorina subgenus Neritrema (Mollusca, Caenogastropoda) includes the “obtusata” group of closely related species (Littorina obtusata and L. fabalis). The anatomy of the adult reproductive system (pallial oviduct) is the only reliable feature used for species identification in females of these species. Reproductive system anatomy and reproduction-associated proteins often diverge between sibling species. Despite being of high evolutionary interest, the molecular basis of this divergence remains poorly understood. We performed proteotranscriptomic comparison of oviducts of L. obtusata and L. fabalis by RNA-seq on Illumina HiSeq 2500 and two-dimensional protein electrophoresis (2D DIGE) with MS/MS identification of the species-specific proteins. The interspecies differences in the oviduct were associated with (1) metabolic proteins reflecting overall physiological differences between L. obtusata and L. fabalis, (2) receptor proteins, and (3) transcripts related to transposable elements (TEs). Various receptors identified may recognize a wide variety of ligands from pathogen-associated molecular patterns to specific carbohydrates on the sperm surface. Therefore, these may participate in immune defense as well as in sperm storage and regulation. Species-specificity of multiple TE sequences (coding for reverse transcriptase and ribonuclease H) may indicate the important role of these genomic elements in the Littorina species divergence, which has not been reported previously.

AB - Genus Littorina subgenus Neritrema (Mollusca, Caenogastropoda) includes the “obtusata” group of closely related species (Littorina obtusata and L. fabalis). The anatomy of the adult reproductive system (pallial oviduct) is the only reliable feature used for species identification in females of these species. Reproductive system anatomy and reproduction-associated proteins often diverge between sibling species. Despite being of high evolutionary interest, the molecular basis of this divergence remains poorly understood. We performed proteotranscriptomic comparison of oviducts of L. obtusata and L. fabalis by RNA-seq on Illumina HiSeq 2500 and two-dimensional protein electrophoresis (2D DIGE) with MS/MS identification of the species-specific proteins. The interspecies differences in the oviduct were associated with (1) metabolic proteins reflecting overall physiological differences between L. obtusata and L. fabalis, (2) receptor proteins, and (3) transcripts related to transposable elements (TEs). Various receptors identified may recognize a wide variety of ligands from pathogen-associated molecular patterns to specific carbohydrates on the sperm surface. Therefore, these may participate in immune defense as well as in sperm storage and regulation. Species-specificity of multiple TE sequences (coding for reverse transcriptase and ribonuclease H) may indicate the important role of these genomic elements in the Littorina species divergence, which has not been reported previously.

KW - 2D DIGE

KW - Invertebrate reproduction

KW - Littorina

KW - Mollusca

KW - RNA-seq

KW - Sibling species

UR - http://www.scopus.com/inward/record.url?scp=85118204602&partnerID=8YFLogxK

UR - https://www.mendeley.com/catalogue/128201da-4f30-3fb1-94d8-56cd0a380ad4/

U2 - 10.3390/biology10111087

DO - 10.3390/biology10111087

M3 - Article

AN - SCOPUS:85118204602

VL - 10

SP - 1087

JO - Biology

JF - Biology

SN - 2079-7737

IS - 11

M1 - 1087

ER -