TY - JOUR

T1 - Solid-liquid extraction into hydrophilic deep eutectic solvent followed by preconcentration into primary amine-based supramolecular solvent: liquid chromatography-mass spectrometry determination of tetracyclines in chicken meat

AU - Курашов, Ярослав Владимирович

AU - Почивалов, Алексей Сергеевич

AU - Фролова, Дарья Александровна

AU - Гадельшин, Руслан Радикович

AU - Гармонов, Сергей

AU - Булатов, Андрей Васильевич

PY - 2025/10/11

Y1 - 2025/10/11

N2 - The phenomenon of supramolecular solvent formation in an aqueous phase containing a primary amine, in the presence of a hydrophilic three-component deep eutectic solvent based on choline chloride, a polar carboxylic acid and water was described for the first time. The established phenomenon was implemented for the preconcentration of polar analytes after their separation from solid samples into the three-component deep eutectic solvent. The mixed supramolecular aggregates were formed by mixing the extract phase containing analytes and deep eutectic solvent precursors with the primary amine. The developed approach was applied to the determination of tetracyclines (minocycline, oxytetracycline, tetracycline, chlortetracycline, and doxycycline) in chicken meat samples by liquid chromatography-mass spectrometry. Various three-component deep eutectic solvents were investigated for the separation of tetracyclines from meat matrices. The most appropriate composition contained choline chloride and glycolic acid at a molar ratio of 1:1 with water (20 %). Effective protein precipitation during solid-liquid extraction from meat samples into the deep eutectic solvent was established. For supramolecular solvent-based liquid-phase microextraction 1-nonylamine was chosen as an amphiphile. Limits of detection of tetracyclines in chicken meat samples were from 1 to 4 μg kg-1.

AB - The phenomenon of supramolecular solvent formation in an aqueous phase containing a primary amine, in the presence of a hydrophilic three-component deep eutectic solvent based on choline chloride, a polar carboxylic acid and water was described for the first time. The established phenomenon was implemented for the preconcentration of polar analytes after their separation from solid samples into the three-component deep eutectic solvent. The mixed supramolecular aggregates were formed by mixing the extract phase containing analytes and deep eutectic solvent precursors with the primary amine. The developed approach was applied to the determination of tetracyclines (minocycline, oxytetracycline, tetracycline, chlortetracycline, and doxycycline) in chicken meat samples by liquid chromatography-mass spectrometry. Various three-component deep eutectic solvents were investigated for the separation of tetracyclines from meat matrices. The most appropriate composition contained choline chloride and glycolic acid at a molar ratio of 1:1 with water (20 %). Effective protein precipitation during solid-liquid extraction from meat samples into the deep eutectic solvent was established. For supramolecular solvent-based liquid-phase microextraction 1-nonylamine was chosen as an amphiphile. Limits of detection of tetracyclines in chicken meat samples were from 1 to 4 μg kg-1.

KW - супрамолекулярные растворители

KW - микроэкстракция

KW - Deep eutectic solvent

KW - HPLC-MS/MS

KW - Liquid-phase microextraction

KW - Solid-liquid extraction

KW - Supramolecular solvent

KW - Tetracyclines

KW - chicken meat

UR - https://www.sciencedirect.com/science/article/abs/pii/S0021967325006168?via%3Dihub

UR - https://www.mendeley.com/catalogue/77589d2d-e7bc-3d78-b474-59927b9697c5/

U2 - 10.1016/j.chroma.2025.466271

DO - 10.1016/j.chroma.2025.466271

M3 - Article

VL - 1760

JO - Journal of Chromatography

JF - Journal of Chromatography

SN - 0021-9673

M1 - 466271

ER -