Research output: Contribution to journal › Article › peer-review
Small-angle X-ray scattering study of the influence of solvent replacement (from H2O to D2O) on the initial crystallization stage of tetragonal lysozyme. / Boikova, A. S.; D’yakova, Yu A.; Il’ina, K. B.; Konarev, P. V.; Kryukova, A. E.; Marchenkova, M. A.; Blagov, A. E.; Pisarevskii, Yu V.; Koval’chuk, M. V.
In: Crystallography Reports, Vol. 62, No. 6, 01.11.2017, p. 837-842.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Small-angle X-ray scattering study of the influence of solvent replacement (from H2O to D2O) on the initial crystallization stage of tetragonal lysozyme
AU - Boikova, A. S.
AU - D’yakova, Yu A.
AU - Il’ina, K. B.
AU - Konarev, P. V.
AU - Kryukova, A. E.
AU - Marchenkova, M. A.
AU - Blagov, A. E.
AU - Pisarevskii, Yu V.
AU - Koval’chuk, M. V.
N1 - Publisher Copyright: © 2017, Pleiades Publishing, Inc.
PY - 2017/11/1
Y1 - 2017/11/1
N2 - The composition of lysozyme solutions in D2O under conditions favorable for the formation of tetragonal crystals has been investigated at different protein concentrations by small-angle X-ray scattering using the synchrotron radiation. In addition to lysozyme monomers, dimeric and octameric species are found in the crystallization solutions; the octamer content increases with an increase in the protein concentration. A comparison of the data with those obtained under similar conditions but with H2O used as a solvent has shown that the replacement of light water with heavy one leads to increase of octamer volume fraction in solution.
AB - The composition of lysozyme solutions in D2O under conditions favorable for the formation of tetragonal crystals has been investigated at different protein concentrations by small-angle X-ray scattering using the synchrotron radiation. In addition to lysozyme monomers, dimeric and octameric species are found in the crystallization solutions; the octamer content increases with an increase in the protein concentration. A comparison of the data with those obtained under similar conditions but with H2O used as a solvent has shown that the replacement of light water with heavy one leads to increase of octamer volume fraction in solution.
UR - http://www.scopus.com/inward/record.url?scp=85034572397&partnerID=8YFLogxK
U2 - 10.1134/S1063774517060074
DO - 10.1134/S1063774517060074
M3 - Article
AN - SCOPUS:85034572397
VL - 62
SP - 837
EP - 842
JO - Crystallography Reports
JF - Crystallography Reports
SN - 1063-7745
IS - 6
ER -
ID: 88201069