TY - JOUR

T1 - Screening for amyloid proteins in the yeast proteome

AU - Ryzhova, Tatyana

AU - Sopova, Julia V

AU - Zadorsky, Sergey P

AU - Siniukova, Vera A

AU - Sergeeva, Aleksandra V

AU - Galkina, Svetlana A

AU - Nizhnikov, Anton A

AU - Shenfeld, Aleksandr A

AU - Volkov, Kirill V

AU - Galkin, Alexey P.

PY - 2018/4/1

Y1 - 2018/4/1

N2 - The search for novel pathological and functional amyloids represents one of the most important tasks of contemporary biomedicine. Formation of pathological amyloid fibrils in the aging brain causes incurable neurodegenerative disorders such as Alzheimer's, Parkinson's Huntington's diseases. At the same time, a set of amyloids regulates vital processes in archaea, prokaryotes and eukaryotes. Our knowledge of the prevalence and biological significance of amyloids is limited due to the lack of universal methods for their identification. Here, using our original method of proteomic screening PSIA-LC-MALDI, we identified a number of proteins that form amyloid-like detergent-resistant aggregates in Saccharomyces cerevisiae. We revealed in yeast strains of different origin known yeast prions, prion-associated proteins, and a set of proteins whose amyloid properties were not shown before. A substantial number of the identified proteins are cell wall components, suggesting that amyloids may play important roles in the formation of this extracellular protective sheath. Two proteins identified in our screen, Gas1 and Ygp1, involved in biogenesis of the yeast cell wall, were selected for detailed analysis of amyloid properties. We show that Gas1 and Ygp1 demonstrate amyloid properties both in vivo in yeast cells and using the bacteria-based system C-DAG. Taken together, our data show that this proteomic approach is very useful for identification of novel amyloids.

AB - The search for novel pathological and functional amyloids represents one of the most important tasks of contemporary biomedicine. Formation of pathological amyloid fibrils in the aging brain causes incurable neurodegenerative disorders such as Alzheimer's, Parkinson's Huntington's diseases. At the same time, a set of amyloids regulates vital processes in archaea, prokaryotes and eukaryotes. Our knowledge of the prevalence and biological significance of amyloids is limited due to the lack of universal methods for their identification. Here, using our original method of proteomic screening PSIA-LC-MALDI, we identified a number of proteins that form amyloid-like detergent-resistant aggregates in Saccharomyces cerevisiae. We revealed in yeast strains of different origin known yeast prions, prion-associated proteins, and a set of proteins whose amyloid properties were not shown before. A substantial number of the identified proteins are cell wall components, suggesting that amyloids may play important roles in the formation of this extracellular protective sheath. Two proteins identified in our screen, Gas1 and Ygp1, involved in biogenesis of the yeast cell wall, were selected for detailed analysis of amyloid properties. We show that Gas1 and Ygp1 demonstrate amyloid properties both in vivo in yeast cells and using the bacteria-based system C-DAG. Taken together, our data show that this proteomic approach is very useful for identification of novel amyloids.

KW - Alzheimer Disease/genetics

KW - Amyloid/genetics

KW - Amyloidogenic Proteins/genetics

KW - Humans

KW - Prion Proteins/genetics

KW - Prokaryotic Cells/metabolism

KW - Proteome/genetics

KW - Proteomics

KW - Saccharomyces cerevisiae/genetics

KW - Yeast

KW - Ygp1

KW - Gas1

KW - Amyloid

KW - Prion

KW - Proteomic screen

KW - SACCHAROMYCES-CEREVISIAE

KW - CELL-WALL

KW - HSP104

KW - MEMBRANE

KW - PRION

KW - IDENTIFICATION

KW - RESPONSES

KW - CHAPERONES

KW - WALL GLUCANTRANSFERASE BGL2P

KW - PROPAGATION

UR - http://www.scopus.com/inward/record.url?scp=85031416256&partnerID=8YFLogxK

UR - http://www.mendeley.com/research/screening-amyloid-proteins-yeast-proteome

U2 - 10.1007/s00294-017-0759-7

DO - 10.1007/s00294-017-0759-7

M3 - Article

C2 - 29027580

VL - 64

SP - 469

EP - 478

JO - Current Genetics

JF - Current Genetics

SN - 0172-8083

IS - 2

ER -