Satellite DNA binding and cellular localisation of RNA helicase P68

Standard

Satellite DNA binding and cellular localisation of RNA helicase P68. / Enukashvily, N; Donev, R; Sheer, D; Podgornaya, O.

In: Journal of Cell Science, Vol. 118, No. 3, 2005, p. 611-622.

Research output: Contribution to journal › Article

Author

Enukashvily, N ; Donev, R ; Sheer, D ; Podgornaya, O. / Satellite DNA binding and cellular localisation of RNA helicase P68. In: Journal of Cell Science. 2005 ; Vol. 118, No. 3. pp. 611-622.

BibTeX

@article{77077addcc2442519b14effa5bcf9c11,

title = "Satellite DNA binding and cellular localisation of RNA helicase P68",

abstract = "We purified a 68-kDa protein from the mouse nuclear matrix using ion exchange and affinity chromatography. Column fractions were tested for specific binding to mouse minor satellite DNA using a gel mobility shift assay. The protein was identified by mass spectrometry as RNA helicase P68. In fixed cells, P68 was found to shuttle in and out of SC35 domains, forming fibres and granules in a cell-cycle dependent manner. Analysis of the P68 sequence revealed a short potential coiled-coil domain that might be involved in the formation of P68 fibres. Contacts between centromeres and P68 granules were observed during all phases of the cycle but they were most prominent in mitosis. At this stage, P68 was found in both the centromeric regions and the connections between chromosomes. Direct interaction of P68/DEAD box RNA helicase with satellite DNAs in vitro has not been demonstrated for any other members of the RNA helicase family.",

keywords = "RNA helicase P68 Satellite DNA Centromere",

author = "N Enukashvily and R Donev and D Sheer and O. Podgornaya",

year = "2005",

doi = "10.1242/jcs.01605",

language = "не определен",

volume = "118",

pages = "611--622",

journal = "Journal of Cell Science",

issn = "0021-9533",

publisher = "Company of Biologists Ltd",

number = "3",

}

RIS

TY - JOUR

T1 - Satellite DNA binding and cellular localisation of RNA helicase P68

AU - Enukashvily, N

AU - Donev, R

AU - Sheer, D

AU - Podgornaya, O.

PY - 2005

Y1 - 2005

N2 - We purified a 68-kDa protein from the mouse nuclear matrix using ion exchange and affinity chromatography. Column fractions were tested for specific binding to mouse minor satellite DNA using a gel mobility shift assay. The protein was identified by mass spectrometry as RNA helicase P68. In fixed cells, P68 was found to shuttle in and out of SC35 domains, forming fibres and granules in a cell-cycle dependent manner. Analysis of the P68 sequence revealed a short potential coiled-coil domain that might be involved in the formation of P68 fibres. Contacts between centromeres and P68 granules were observed during all phases of the cycle but they were most prominent in mitosis. At this stage, P68 was found in both the centromeric regions and the connections between chromosomes. Direct interaction of P68/DEAD box RNA helicase with satellite DNAs in vitro has not been demonstrated for any other members of the RNA helicase family.

AB - We purified a 68-kDa protein from the mouse nuclear matrix using ion exchange and affinity chromatography. Column fractions were tested for specific binding to mouse minor satellite DNA using a gel mobility shift assay. The protein was identified by mass spectrometry as RNA helicase P68. In fixed cells, P68 was found to shuttle in and out of SC35 domains, forming fibres and granules in a cell-cycle dependent manner. Analysis of the P68 sequence revealed a short potential coiled-coil domain that might be involved in the formation of P68 fibres. Contacts between centromeres and P68 granules were observed during all phases of the cycle but they were most prominent in mitosis. At this stage, P68 was found in both the centromeric regions and the connections between chromosomes. Direct interaction of P68/DEAD box RNA helicase with satellite DNAs in vitro has not been demonstrated for any other members of the RNA helicase family.

KW - RNA helicase P68 Satellite DNA Centromere

U2 - 10.1242/jcs.01605

DO - 10.1242/jcs.01605

M3 - статья

VL - 118

SP - 611

EP - 622

JO - Journal of Cell Science

JF - Journal of Cell Science

SN - 0021-9533

IS - 3

ER -

ID: 5454698