Research output: Contribution to journal › Article › peer-review
Rhizobia isolated from the relict legume vavilovia formosa represent a genetically specific group within rhizobium leguminosarum biovar viciae. / Kimeklis, Anastasiia K.; Chirak, Elizaveta R.; Kuznetsova, Irina G.; Sazanova, Anna L.; Safronova, Vera I.; Belimov, Andrey A.; Onishchuk, Olga P.; Kurchak, Oksana N.; Aksenova, Тatyana S.; Pinaev, Alexander G.; Andronov, Evgeny E.; Provorov, Nikolay A.
In: Genes, Vol. 10, No. 12, 991, 12.2019.Research output: Contribution to journal › Article › peer-review
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TY - JOUR
T1 - Rhizobia isolated from the relict legume vavilovia formosa represent a genetically specific group within rhizobium leguminosarum biovar viciae
AU - Kimeklis, Anastasiia K.
AU - Chirak, Elizaveta R.
AU - Kuznetsova, Irina G.
AU - Sazanova, Anna L.
AU - Safronova, Vera I.
AU - Belimov, Andrey A.
AU - Onishchuk, Olga P.
AU - Kurchak, Oksana N.
AU - Aksenova, Тatyana S.
AU - Pinaev, Alexander G.
AU - Andronov, Evgeny E.
AU - Provorov, Nikolay A.
N1 - Publisher Copyright: © 2019 by the authors. Licensee MDPI, Basel, Switzerland.
PY - 2019/12
Y1 - 2019/12
N2 - Twenty-two rhizobia strains isolated from three distinct populations (North Ossetia, Dagestan, and Armenia) of a relict legume Vavilovia formosa were analysed to determine their position within Rhizobium leguminosarum biovar viciae (Rlv). These bacteria are described as symbionts of four plant genera Pisum, Vicia, Lathyrus, and Lens from the Fabeae tribe, of which Vavilovia is considered to be closest to its last common ancestor (LCA). In contrast to biovar viciae, bacteria from Rhizobium leguminosarum biovar trifolii (Rlt) inoculate plants from the Trifolieae tribe. Comparison of house-keeping (hkg: 16S rRNA, glnII, gltA, and dnaK) and symbiotic (sym: nodA, nodC, nodD, and nifH) genes of the symbionts of V. formosa with those of other Rlv and Rlt strains reveals a significant group separation, which was most pronounced for sym genes. A remarkable feature of the strains isolated from V. formosa was the presence of the nodX gene, which was commonly found in Rlv strains isolated from Afghanistan pea genotypes. Tube testing of different strains on nine plant species, including all genera from the Fabeae tribe, demonstrated that the strains from V. formosa nodulated the same cross inoculation group as the other Rlv strains. Comparison of nucleotide similarity in sym genes suggested that their diversification within sym-biotypes of Rlv was elicited by host plants. Contrariwise, that of hkg genes could be caused by either local adaptation to soil niches or by genetic drift. Long-term ecological isolation, genetic separation, and the ancestral position of V. formosa suggested that symbionts of V. formosa could be responsible for preserving ancestral genotypes of the Rlv biovar.
AB - Twenty-two rhizobia strains isolated from three distinct populations (North Ossetia, Dagestan, and Armenia) of a relict legume Vavilovia formosa were analysed to determine their position within Rhizobium leguminosarum biovar viciae (Rlv). These bacteria are described as symbionts of four plant genera Pisum, Vicia, Lathyrus, and Lens from the Fabeae tribe, of which Vavilovia is considered to be closest to its last common ancestor (LCA). In contrast to biovar viciae, bacteria from Rhizobium leguminosarum biovar trifolii (Rlt) inoculate plants from the Trifolieae tribe. Comparison of house-keeping (hkg: 16S rRNA, glnII, gltA, and dnaK) and symbiotic (sym: nodA, nodC, nodD, and nifH) genes of the symbionts of V. formosa with those of other Rlv and Rlt strains reveals a significant group separation, which was most pronounced for sym genes. A remarkable feature of the strains isolated from V. formosa was the presence of the nodX gene, which was commonly found in Rlv strains isolated from Afghanistan pea genotypes. Tube testing of different strains on nine plant species, including all genera from the Fabeae tribe, demonstrated that the strains from V. formosa nodulated the same cross inoculation group as the other Rlv strains. Comparison of nucleotide similarity in sym genes suggested that their diversification within sym-biotypes of Rlv was elicited by host plants. Contrariwise, that of hkg genes could be caused by either local adaptation to soil niches or by genetic drift. Long-term ecological isolation, genetic separation, and the ancestral position of V. formosa suggested that symbionts of V. formosa could be responsible for preserving ancestral genotypes of the Rlv biovar.
KW - Evolution of symbiosis
KW - Group separation
KW - Housekeeping genes (hkg)
KW - Rhizobium leguminosarum bv. Viciae
KW - Symbiotic (sym) genes
KW - Tribe Fabeae
KW - Vavilovia formosa (Stev.) Fed
UR - http://www.scopus.com/inward/record.url?scp=85076272274&partnerID=8YFLogxK
U2 - 10.3390/genes10120991
DO - 10.3390/genes10120991
M3 - Article
C2 - 31805683
AN - SCOPUS:85076272274
VL - 10
JO - Genes
JF - Genes
SN - 2073-4425
IS - 12
M1 - 991
ER -
ID: 95576708