Research output: Contribution to journal › Conference article › peer-review
Regulation of the synaptic vesicle liquid phase by dynamic SH3 domain binding. / Brodin, Lennart; Sopova, E. ; Gerth, Fabian; Freund, Christian; Shupliakov, O. .
In: European Neuropsychopharmacology, Vol. 53 , No. Supplement 1, 12.2021, p. S205.Research output: Contribution to journal › Conference article › peer-review
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TY - JOUR
T1 - Regulation of the synaptic vesicle liquid phase by dynamic SH3 domain binding
AU - Brodin, Lennart
AU - Sopova, E.
AU - Gerth, Fabian
AU - Freund, Christian
AU - Shupliakov, O.
PY - 2021/12
Y1 - 2021/12
N2 - Synaptic vesicle (SV) clusters are essential for neurotransmitter release at high rates of stimulation [1] with a role in pathological states, such as epilepsy. It was proposed that the resting SV cluster is organized as a liquid phase maintained by interactions of the intrinsically disordered region (IDR) of synapsin. To address how the SV liquid phase is regulated during synaptic activity we used intersectin (ITSN) as model protein. Intersectin, which is accumulated in the SV cluster, interacts with the synapsin IDR through a conserved phospho-regulated switch mechanism. In dephospho-intersectin, synapsin-binding to an ITSN SH3 domain is autoinhibited while in phospho-intersectin it strongly binds synapsin.
AB - Synaptic vesicle (SV) clusters are essential for neurotransmitter release at high rates of stimulation [1] with a role in pathological states, such as epilepsy. It was proposed that the resting SV cluster is organized as a liquid phase maintained by interactions of the intrinsically disordered region (IDR) of synapsin. To address how the SV liquid phase is regulated during synaptic activity we used intersectin (ITSN) as model protein. Intersectin, which is accumulated in the SV cluster, interacts with the synapsin IDR through a conserved phospho-regulated switch mechanism. In dephospho-intersectin, synapsin-binding to an ITSN SH3 domain is autoinhibited while in phospho-intersectin it strongly binds synapsin.
U2 - 10.1016/j.euroneuro.2021.10.269
DO - 10.1016/j.euroneuro.2021.10.269
M3 - Conference article
VL - 53
SP - S205
JO - European Neuropsychopharmacology
JF - European Neuropsychopharmacology
SN - 0924-977X
IS - Supplement 1
Y2 - 2 October 2021 through 5 October 2021
ER -
ID: 83997307