TY - JOUR

T1 - Regulation of the later stages of nodulation stimulated by IPD3/CYCLOPS transcription factor and cytokinin in pea Pisum sativum L.

AU - Rudaya, Elizaveta S.

AU - Kozyulina, Polina Yu

AU - Pavlova, Olga A.

AU - Dolgikh, Alexandra V.

AU - Ivanova, Alexandra N.

AU - Dolgikh, Elena A.

N1 - Rudaya, E.S.; Kozyulina, P.Y.; Pavlova, O.A.; Dolgikh, A.V.; Ivanova, A.N.; Dolgikh, E.A. Regulation of the Later Stages of Nodulation Stimulated by IPD3/CYCLOPS Transcription Factor and Cytokinin in Pea Pisum sativum L. Plants 2022, 11, 56. https://doi.org/10.3390/plants11010056

PY - 2022/1/1

Y1 - 2022/1/1

N2 - The IPD3/CYCLOPS transcription factor was shown to be involved in the regulation of nodule primordia development and subsequent stages of nodule differentiation. In contrast to early stages, the stages related to nodule differentiation remain less studied. Recently, we have shown that the accumulation of cytokinin at later stages may significantly impact nodule development. This conclusion was based on a comparative analysis of cytokinin localization between pea wild type and ipd3/cyclops mutants. However, the role of cytokinin at these later stages of nodulation is still far from understood. To determine a set of genes involved in the regulation of later stages of nodule development connected with infection progress, intracellular accommodation, as well as plant tissue and bacteroid differentiation, the RNA-seq analysis of pea mutant SGEFix--2 (sym33) nodules impaired in these processes compared to wild type SGE nodules was performed. To verify cytokinin’s influence on late nodule development stages, the comparative RNA-seq analysis of SGEFix--2 (sym33) mutant plants treated with cytokinin was also conducted. Findings suggest a significant role of cytokinin in the regulation of later stages of nodule development.

AB - The IPD3/CYCLOPS transcription factor was shown to be involved in the regulation of nodule primordia development and subsequent stages of nodule differentiation. In contrast to early stages, the stages related to nodule differentiation remain less studied. Recently, we have shown that the accumulation of cytokinin at later stages may significantly impact nodule development. This conclusion was based on a comparative analysis of cytokinin localization between pea wild type and ipd3/cyclops mutants. However, the role of cytokinin at these later stages of nodulation is still far from understood. To determine a set of genes involved in the regulation of later stages of nodule development connected with infection progress, intracellular accommodation, as well as plant tissue and bacteroid differentiation, the RNA-seq analysis of pea mutant SGEFix--2 (sym33) nodules impaired in these processes compared to wild type SGE nodules was performed. To verify cytokinin’s influence on late nodule development stages, the comparative RNA-seq analysis of SGEFix--2 (sym33) mutant plants treated with cytokinin was also conducted. Findings suggest a significant role of cytokinin in the regulation of later stages of nodule development.

KW - Cytokinin

KW - Gene expression

KW - IPD3/CYCLOPS transcription factor

KW - Pisum sativum

KW - Transcriptomic data

KW - NODULE DEVELOPMENT

KW - RECEPTOR KINASE GENE

KW - CYCLOPS transcription factor

KW - RHIZOBIUM-LEGUMINOSARUM

KW - DEPENDENT PROTEIN-KINASE

KW - transcriptomic data

KW - ROOT HAIRS

KW - LOTUS-JAPONICUS

KW - cytokinin

KW - IPD3

KW - LEGUME NODULE

KW - MEDICAGO-TRUNCATULA

KW - INFECTION

KW - EXPRESSION

KW - gene expression

UR - http://www.scopus.com/inward/record.url?scp=85122334115&partnerID=8YFLogxK

U2 - 10.3390/plants11010056

DO - 10.3390/plants11010056

M3 - Article

AN - SCOPUS:85122334115

VL - 11

JO - Plants

JF - Plants

SN - 2223-7747

IS - 1

M1 - 56

ER -