Regulation of Apolipoprotein A-I Gene Expression in Human Macrophages by Oxidized Low-Density Lipoprotein

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Regulation of Apolipoprotein A-I Gene Expression in Human Macrophages by Oxidized Low-Density Lipoprotein. / Nekrasova, Ekaterina V.; Larionova, Ekaterina E.; Danko, Katerina ; Kuzmina, Darya O.; Shavva, Vladimir S.; Kudriavtsev, Igor V.; Orlov, Sergey V.

In: Biochemistry (Moscow), Vol. 86, No. 10, 10.2021, p. 1201-1213.

Research output: Contribution to journal › Article › peer-review

Author

Nekrasova, Ekaterina V. ; Larionova, Ekaterina E. ; Danko, Katerina ; Kuzmina, Darya O. ; Shavva, Vladimir S. ; Kudriavtsev, Igor V. ; Orlov, Sergey V. / Regulation of Apolipoprotein A-I Gene Expression in Human Macrophages by Oxidized Low-Density Lipoprotein. In: Biochemistry (Moscow). 2021 ; Vol. 86, No. 10. pp. 1201-1213.

BibTeX

@article{7cf2aabd0fa04903b3ccb715a09298f4,

title = "Regulation of Apolipoprotein A-I Gene Expression in Human Macrophages by Oxidized Low-Density Lipoprotein",

abstract = "Abstract: Apolipoprotein A-I (ApoA-I) is a key component of reverse cholesterol transport in humans. In the previous studies, we demonstrated expression of the apoA-I gene in human monocytes and macrophages; however, little is known on the regulation of the apoA-I expression in macrophages during the uptake of modified low-density lipoprotein (LDL), which is one of the key processes in the early stages of atherogenesis leading to formation of foam cells. Here, we demonstrate a complex nature of the apoA-I regulation in human macrophages during the uptake of oxidized LDL (oxLDL). Incubation of macrophages with oxLDL induced expression of the apoA-I gene within the first 24 hours, but suppressed it after 48 h. Both effects depended on the interaction of oxLDL with the TLR4 receptor, rather than on the oxLDL uptake by the macrophages. The oxLDL-mediated downregulation of the apoA-I gene depended on the ERK1/2 and JNK cascades, as well as on the NF-κB cascade.",

keywords = "apoA-I gene, atherosclerosis, foam cells, gene expression regulation, macrophages, oxLDL, THP-1 cells, TLR4",

author = "Nekrasova, {Ekaterina V.} and Larionova, {Ekaterina E.} and Katerina Danko and Kuzmina, {Darya O.} and Shavva, {Vladimir S.} and Kudriavtsev, {Igor V.} and Orlov, {Sergey V.}",

note = "Publisher Copyright: {\textcopyright} 2021, Pleiades Publishing, Ltd.",

year = "2021",

month = oct,

doi = "10.1134/S0006297921100047",

language = "English",

volume = "86",

pages = "1201--1213",

journal = "Biochemistry (Moscow)",

issn = "0006-2979",

publisher = "МАИК {"}Наука/Интерпериодика{"}",

number = "10",

}

RIS

TY - JOUR

T1 - Regulation of Apolipoprotein A-I Gene Expression in Human Macrophages by Oxidized Low-Density Lipoprotein

AU - Nekrasova, Ekaterina V.

AU - Larionova, Ekaterina E.

AU - Danko, Katerina

AU - Kuzmina, Darya O.

AU - Shavva, Vladimir S.

AU - Kudriavtsev, Igor V.

AU - Orlov, Sergey V.

PY - 2021/10

Y1 - 2021/10

N2 - Abstract: Apolipoprotein A-I (ApoA-I) is a key component of reverse cholesterol transport in humans. In the previous studies, we demonstrated expression of the apoA-I gene in human monocytes and macrophages; however, little is known on the regulation of the apoA-I expression in macrophages during the uptake of modified low-density lipoprotein (LDL), which is one of the key processes in the early stages of atherogenesis leading to formation of foam cells. Here, we demonstrate a complex nature of the apoA-I regulation in human macrophages during the uptake of oxidized LDL (oxLDL). Incubation of macrophages with oxLDL induced expression of the apoA-I gene within the first 24 hours, but suppressed it after 48 h. Both effects depended on the interaction of oxLDL with the TLR4 receptor, rather than on the oxLDL uptake by the macrophages. The oxLDL-mediated downregulation of the apoA-I gene depended on the ERK1/2 and JNK cascades, as well as on the NF-κB cascade.

AB - Abstract: Apolipoprotein A-I (ApoA-I) is a key component of reverse cholesterol transport in humans. In the previous studies, we demonstrated expression of the apoA-I gene in human monocytes and macrophages; however, little is known on the regulation of the apoA-I expression in macrophages during the uptake of modified low-density lipoprotein (LDL), which is one of the key processes in the early stages of atherogenesis leading to formation of foam cells. Here, we demonstrate a complex nature of the apoA-I regulation in human macrophages during the uptake of oxidized LDL (oxLDL). Incubation of macrophages with oxLDL induced expression of the apoA-I gene within the first 24 hours, but suppressed it after 48 h. Both effects depended on the interaction of oxLDL with the TLR4 receptor, rather than on the oxLDL uptake by the macrophages. The oxLDL-mediated downregulation of the apoA-I gene depended on the ERK1/2 and JNK cascades, as well as on the NF-κB cascade.

KW - apoA-I gene

KW - atherosclerosis

KW - foam cells

KW - gene expression regulation

KW - macrophages

KW - oxLDL

KW - THP-1 cells

KW - TLR4

UR - http://www.scopus.com/inward/record.url?scp=85115076410&partnerID=8YFLogxK

U2 - 10.1134/S0006297921100047

DO - 10.1134/S0006297921100047

M3 - Article

AN - SCOPUS:85115076410

VL - 86

SP - 1201

EP - 1213

JO - Biochemistry (Moscow)

JF - Biochemistry (Moscow)

SN - 0006-2979

IS - 10

ER -

ID: 91160221