TY - JOUR

T1 - Recovery process in sponges: morphogenesis and cell sources

AU - Lavrov, Andrey

AU - Bolshakov , Fyodor

AU - Borisenko, Ilya

AU - Frolova, Veronika

AU - Tokina, Daria

AU - Ereskovsky, Alexander

N1 - ISJ – Invertebrate Survival Journal. 17:28-29.

PY - 2020/2

Y1 - 2020/2

N2 - Sponges (Porifera) represent one of the most ancient metazoan lineages. They possess unique anatomical and tissue structure, making them promising models for evolutionary studies. The high plasticity of sponge tissue and cells provide them with outstanding recovery abilities, ranging from wound healing to re-building of a functional body from dissociated cells.We have combined several microscopy techniques to elucidate morphogeneses, cellular mechanisms and cell sources during reparative regeneration and cell reaggregation in five species from different clades: Halisarca dujardinii (Demospongiae), Aplysina cavernicola (Demosponigae), Sycon sp. (Calcarea), Leucosolenia variabilis (Calcarea) and Clathrina arnesenae (Calcarea).The main mechanism of reparative regeneration in studied Demospongiae is cell migrations and epithelio-mesenchymal transformations, involving archaeocytes and choanocytes, which are a cell source for the recovery of lost structures. In contrast, the reparative regeneration in Calcarea occurs due to extensive remodeling of intact tissues near the wound through epithelial morphogeneses, accompanied by cell transdifferentiations.The cell reaggregation in both Demospongiae and Calcarea involves mass cell dedifferentiation on the early stages of the process. During progressive development of multicellular aggregates, individual cell migrations and transdifferentiations ensure restoration of required cell types and intact anatomical structures. However, epithelial morphogeneses contribute to the development of calcareous sponge aggregates.Thus, during recovery processes, sponges utilize diverse and complex morphogenetic mechanisms, with a particular importance of cell transdifferentiation. While all sponges demonstrate high recovery abilities, the morphogeneses and cell sources for the recovery of lost structures varies in different clades.The study was support by RFBR nos.16-04-00084, 16-34-00145, 19-04-00545 and 19-04-00563 and RSF no.17-14-01089.

AB - Sponges (Porifera) represent one of the most ancient metazoan lineages. They possess unique anatomical and tissue structure, making them promising models for evolutionary studies. The high plasticity of sponge tissue and cells provide them with outstanding recovery abilities, ranging from wound healing to re-building of a functional body from dissociated cells.We have combined several microscopy techniques to elucidate morphogeneses, cellular mechanisms and cell sources during reparative regeneration and cell reaggregation in five species from different clades: Halisarca dujardinii (Demospongiae), Aplysina cavernicola (Demosponigae), Sycon sp. (Calcarea), Leucosolenia variabilis (Calcarea) and Clathrina arnesenae (Calcarea).The main mechanism of reparative regeneration in studied Demospongiae is cell migrations and epithelio-mesenchymal transformations, involving archaeocytes and choanocytes, which are a cell source for the recovery of lost structures. In contrast, the reparative regeneration in Calcarea occurs due to extensive remodeling of intact tissues near the wound through epithelial morphogeneses, accompanied by cell transdifferentiations.The cell reaggregation in both Demospongiae and Calcarea involves mass cell dedifferentiation on the early stages of the process. During progressive development of multicellular aggregates, individual cell migrations and transdifferentiations ensure restoration of required cell types and intact anatomical structures. However, epithelial morphogeneses contribute to the development of calcareous sponge aggregates.Thus, during recovery processes, sponges utilize diverse and complex morphogenetic mechanisms, with a particular importance of cell transdifferentiation. While all sponges demonstrate high recovery abilities, the morphogeneses and cell sources for the recovery of lost structures varies in different clades.The study was support by RFBR nos.16-04-00084, 16-34-00145, 19-04-00545 and 19-04-00563 and RSF no.17-14-01089.

UR - https://f1000research.com/posters/8-490

UR - https://hal.archives-ouvertes.fr/hal-02354447/document

U2 - 10.25431/1824-307X/isj.v0i0.24-31

DO - 10.25431/1824-307X/isj.v0i0.24-31

M3 - Meeting Abstract

VL - 176

SP - 28

EP - 29

JO - Invertebrate Survival Journal

JF - Invertebrate Survival Journal

SN - 1824-307X

T2 - 2nd MARISTEM Workshop - Omics approaches to identify and characterize marine/aquatic invertebrate stem cells

Y2 - 8 April 2019 through 11 April 2019

ER -