Reconstruction of phylogenetic relationships in dermatomycete genus Trichophyton Malmsten 1848 based on ribosomal internal transcribed spacer region, partial 28S rRNA and beta-tubulin genes sequences

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Reconstruction of phylogenetic relationships in dermatomycete genus Trichophyton Malmsten 1848 based on ribosomal internal transcribed spacer region, partial 28S rRNA and beta-tubulin genes sequences. / Pchelin, Ivan M.; Zlatogursky, Vasily V.; Rudneva, Mariya V.; Chilina, Galina A.; Rezaei-Matehkolaei, Ali; Lavnikevich, Dmitry M.; Vasilyeva, Natalya V.; Taraskina, Anastasia E.

In: Mycoses, Vol. 59, No. 9, 01.09.2016, p. 566-575.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{7d9ab6391220494ab9352bb9f90dbb2b,

title = "Reconstruction of phylogenetic relationships in dermatomycete genus Trichophyton Malmsten 1848 based on ribosomal internal transcribed spacer region, partial 28S rRNA and beta-tubulin genes sequences",

abstract = "Trichophyton spp. are important causative agents of superficial mycoses. The phylogeny of the genus and accurate strain identification, based on the ribosomal ITS region sequencing, are still under development. The present work is aimed at (i) inferring the genus phylogeny from partial ITS, LSU and BT2 sequences (ii) description of ribosomal ITS region polymorphism in 15 strains of Trichophyton interdigitale. We performed DNA sequence-based species identification and phylogenetic analysis on 48 strains belonging to the genus Trichophyton. Phylogenetic relationships were inferred by maximum likelihood and Bayesian methods on concatenated ITS, LSU and BT2 sequences. Ribosomal ITS region polymorphisms were assessed directly on the alignment. By phylogenetic reconstruction, we reveal major anthropophilic and zoophilic species clusters in the genus Trichophyton. We describe several sequences of the ITS region of T. interdigitale, which do not fit in the traditional polymorphism scheme and propose emendations in this scheme for discrimination between ITS sequence types in T. interdigitale. The new polymorphism scheme will allow inclusion of a wider spectrum of isolates while retaining its explanatory power. This scheme was also found to be partially congruent with NTS typing technique.",

keywords = "28S rRNA, beta-tubulin, Dermatophyte, internal transcribed spacer, phylogeny, Trichophyton",

author = "Pchelin, {Ivan M.} and Zlatogursky, {Vasily V.} and Rudneva, {Mariya V.} and Chilina, {Galina A.} and Ali Rezaei-Matehkolaei and Lavnikevich, {Dmitry M.} and Vasilyeva, {Natalya V.} and Taraskina, {Anastasia E.}",

note = "Publisher Copyright: {\textcopyright} 2016 Blackwell Verlag GmbH",

year = "2016",

month = sep,

day = "1",

doi = "10.1111/myc.12505",

language = "English",

volume = "59",

pages = "566--575",

journal = "Mycoses",

issn = "0933-7407",

publisher = "Wiley-Blackwell",

number = "9",

}

RIS

TY - JOUR

T1 - Reconstruction of phylogenetic relationships in dermatomycete genus Trichophyton Malmsten 1848 based on ribosomal internal transcribed spacer region, partial 28S rRNA and beta-tubulin genes sequences

AU - Pchelin, Ivan M.

AU - Zlatogursky, Vasily V.

AU - Rudneva, Mariya V.

AU - Chilina, Galina A.

AU - Rezaei-Matehkolaei, Ali

AU - Lavnikevich, Dmitry M.

AU - Vasilyeva, Natalya V.

AU - Taraskina, Anastasia E.

PY - 2016/9/1

Y1 - 2016/9/1

N2 - Trichophyton spp. are important causative agents of superficial mycoses. The phylogeny of the genus and accurate strain identification, based on the ribosomal ITS region sequencing, are still under development. The present work is aimed at (i) inferring the genus phylogeny from partial ITS, LSU and BT2 sequences (ii) description of ribosomal ITS region polymorphism in 15 strains of Trichophyton interdigitale. We performed DNA sequence-based species identification and phylogenetic analysis on 48 strains belonging to the genus Trichophyton. Phylogenetic relationships were inferred by maximum likelihood and Bayesian methods on concatenated ITS, LSU and BT2 sequences. Ribosomal ITS region polymorphisms were assessed directly on the alignment. By phylogenetic reconstruction, we reveal major anthropophilic and zoophilic species clusters in the genus Trichophyton. We describe several sequences of the ITS region of T. interdigitale, which do not fit in the traditional polymorphism scheme and propose emendations in this scheme for discrimination between ITS sequence types in T. interdigitale. The new polymorphism scheme will allow inclusion of a wider spectrum of isolates while retaining its explanatory power. This scheme was also found to be partially congruent with NTS typing technique.

AB - Trichophyton spp. are important causative agents of superficial mycoses. The phylogeny of the genus and accurate strain identification, based on the ribosomal ITS region sequencing, are still under development. The present work is aimed at (i) inferring the genus phylogeny from partial ITS, LSU and BT2 sequences (ii) description of ribosomal ITS region polymorphism in 15 strains of Trichophyton interdigitale. We performed DNA sequence-based species identification and phylogenetic analysis on 48 strains belonging to the genus Trichophyton. Phylogenetic relationships were inferred by maximum likelihood and Bayesian methods on concatenated ITS, LSU and BT2 sequences. Ribosomal ITS region polymorphisms were assessed directly on the alignment. By phylogenetic reconstruction, we reveal major anthropophilic and zoophilic species clusters in the genus Trichophyton. We describe several sequences of the ITS region of T. interdigitale, which do not fit in the traditional polymorphism scheme and propose emendations in this scheme for discrimination between ITS sequence types in T. interdigitale. The new polymorphism scheme will allow inclusion of a wider spectrum of isolates while retaining its explanatory power. This scheme was also found to be partially congruent with NTS typing technique.

KW - 28S rRNA

KW - beta-tubulin

KW - Dermatophyte

KW - internal transcribed spacer

KW - phylogeny

KW - Trichophyton

UR - http://www.scopus.com/inward/record.url?scp=84983567910&partnerID=8YFLogxK

U2 - 10.1111/myc.12505

DO - 10.1111/myc.12505

M3 - Article

C2 - 27071492

AN - SCOPUS:84983567910

VL - 59

SP - 566

EP - 575

JO - Mycoses

JF - Mycoses

SN - 0933-7407

IS - 9

ER -

ID: 99808105