TY - JOUR

T1 - Receptor-like kinase LYK9 in Pisum sativum L. Is the CERK1-like receptor that controls both plant immunity and AM symbiosis development

AU - Leppyanen, Irina V.

AU - Shakhnazarova, Vlada Y.

AU - Shtark, Oksana Y.

AU - Vishnevskaya, Nadezhda A.

AU - Tikhonovich, Igor A.

AU - Dolgikh, Elena A.

N1 - Funding Information: Acknowledgments: This work was supported by Russian Science Foundation (RSF project No. 16-16-10043). The research was performed using the equipment of the Core Center “Genomic technologies, proteomics and cellular biology”. English-language editing of this manuscript was provided by Journal Prep. Publisher Copyright: © 2017 by the authors. Licensee MDPI, Basel, Switzerland. Copyright: Copyright 2018 Elsevier B.V., All rights reserved.

PY - 2018/1

Y1 - 2018/1

N2 - Plants are able to discriminate and respond to structurally related chitooligosaccharide (CO) signals from pathogenic and symbiotic fungi. In model plants Arabidopsis thaliana and Oryza sativa LysM-receptor like kinases (LysM-RLK) AtCERK1 and OsCERK1 (chitin elicitor receptor kinase 1) were shown to be involved in response to CO signals. Based on phylogenetic analysis, the pea Pisum sativum L. LysM-RLK PsLYK9 was chosen as a possible candidate given its role on the CERK1-like receptor. The knockdown regulation of the PsLyk9 gene by RNA interference led to increased susceptibility to fungal pathogen Fusarium culmorum. Transcript levels of PsPAL2, PsPR10 defense-response genes were significantly reduced in PsLyk9 RNAi roots. PsLYK9‘s involvement in recognizing short-chain COs as most numerous signals of arbuscular mycorrhizal (AM) fungi, was also evaluated. In transgenic roots with PsLyk9 knockdown treated with short-chain CO5, downregulation of AM symbiosis marker genes (PsDELLA3, PsNSP2, PsDWARF27) was observed. These results clearly indicate that PsLYK9 appears to be involved in the perception of COs and subsequent signal transduction in pea roots. It allows us to conclude that PsLYK9 is the most likely CERK1-like receptor in pea to be involved in the control of plant immunity and AM symbiosis formation.

AB - Plants are able to discriminate and respond to structurally related chitooligosaccharide (CO) signals from pathogenic and symbiotic fungi. In model plants Arabidopsis thaliana and Oryza sativa LysM-receptor like kinases (LysM-RLK) AtCERK1 and OsCERK1 (chitin elicitor receptor kinase 1) were shown to be involved in response to CO signals. Based on phylogenetic analysis, the pea Pisum sativum L. LysM-RLK PsLYK9 was chosen as a possible candidate given its role on the CERK1-like receptor. The knockdown regulation of the PsLyk9 gene by RNA interference led to increased susceptibility to fungal pathogen Fusarium culmorum. Transcript levels of PsPAL2, PsPR10 defense-response genes were significantly reduced in PsLyk9 RNAi roots. PsLYK9‘s involvement in recognizing short-chain COs as most numerous signals of arbuscular mycorrhizal (AM) fungi, was also evaluated. In transgenic roots with PsLyk9 knockdown treated with short-chain CO5, downregulation of AM symbiosis marker genes (PsDELLA3, PsNSP2, PsDWARF27) was observed. These results clearly indicate that PsLYK9 appears to be involved in the perception of COs and subsequent signal transduction in pea roots. It allows us to conclude that PsLYK9 is the most likely CERK1-like receptor in pea to be involved in the control of plant immunity and AM symbiosis formation.

KW - AM symbiosis

KW - Agrobacterium rhizogenes

KW - Gene expression

KW - Knockdown regulation

KW - Lysin-motif receptor-like kinase PsLYK9

KW - Pea Pisum sativum L.

KW - Phytopathogenic fungi

UR - http://www.scopus.com/inward/record.url?scp=85039698638&partnerID=8YFLogxK

U2 - 10.3390/ijms19010008

DO - 10.3390/ijms19010008

M3 - Article

C2 - 29267197

AN - SCOPUS:85039698638

VL - 19

JO - International Journal of Molecular Sciences

JF - International Journal of Molecular Sciences

SN - 1422-0067

IS - 1

M1 - 8

ER -