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Reassortment between avian apathogenic and human attenuated cold-adapted viruses as an approach for preparing influenza pandemic vaccines. / Desheva, JA; Rudenko, LG; Alexandrova, GI; Lu, C.D.; Rekstin, AR; Katz, JM; Cox, NJ; Klimov, AI.

OPTIONS FOR THE CONTROL OF INFLUENZA V. ed. / Y Kawaoka. Elsevier, 2004. p. 724-727 (INTERNATIONAL CONGRESS SERIES; Vol. 1263).

Research output: Chapter in Book/Report/Conference proceeding › Conference contribution › Research › peer-review

Harvard

Desheva, JA, Rudenko, LG, Alexandrova, GI, Lu, CD, Rekstin, AR, Katz, JM, Cox, NJ & Klimov, AI 2004, Reassortment between avian apathogenic and human attenuated cold-adapted viruses as an approach for preparing influenza pandemic vaccines. in Y Kawaoka (ed.), OPTIONS FOR THE CONTROL OF INFLUENZA V. INTERNATIONAL CONGRESS SERIES, vol. 1263, Elsevier, pp. 724-727, 5th International Conference on Options for the Control of Influenza, Okinawa, Japan, 7/10/03. https://doi.org/10.1016/j.ics.2004.02.043

APA

Desheva, JA., Rudenko, LG., Alexandrova, GI., Lu, C. D., Rekstin, AR., Katz, JM., Cox, NJ., & Klimov, AI. (2004). Reassortment between avian apathogenic and human attenuated cold-adapted viruses as an approach for preparing influenza pandemic vaccines. In Y. Kawaoka (Ed.), OPTIONS FOR THE CONTROL OF INFLUENZA V (pp. 724-727). (INTERNATIONAL CONGRESS SERIES; Vol. 1263). Elsevier. https://doi.org/10.1016/j.ics.2004.02.043

Vancouver

Desheva JA, Rudenko LG, Alexandrova GI, Lu CD, Rekstin AR, Katz JM et al. Reassortment between avian apathogenic and human attenuated cold-adapted viruses as an approach for preparing influenza pandemic vaccines. In Kawaoka Y, editor, OPTIONS FOR THE CONTROL OF INFLUENZA V. Elsevier. 2004. p. 724-727. (INTERNATIONAL CONGRESS SERIES). https://doi.org/10.1016/j.ics.2004.02.043

Author

Desheva, JA ; Rudenko, LG ; Alexandrova, GI ; Lu, C.D. ; Rekstin, AR ; Katz, JM ; Cox, NJ ; Klimov, AI. / Reassortment between avian apathogenic and human attenuated cold-adapted viruses as an approach for preparing influenza pandemic vaccines. OPTIONS FOR THE CONTROL OF INFLUENZA V. editor / Y Kawaoka. Elsevier, 2004. pp. 724-727 (INTERNATIONAL CONGRESS SERIES).

BibTeX

@inproceedings{a1fae93182d5437fbbe3d073500e818d,
title = "Reassortment between avian apathogenic and human attenuated cold-adapted viruses as an approach for preparing influenza pandemic vaccines",
abstract = "To prepare candidate influenza pandemic vaccines, we are developing an approach based on reassortment of antigenically appropriate nonpathogenic avian viruses of different subtypes (H5, H9, H7) with the cold-adapted master strain (MS) A/Leningrad/134/17/57 (Len/17) that is currently used in Russia for preparing licensed live attenuated vaccines for adults and children. In the present study, reassortants between A/Duck/Potsdam/1402-6/86(H5N2) (H5N2-wt) and Len/17 were obtained. One of the clones, A/17/Duck/Potsdam/86-92(H5N2) (Len17/H5), was chosen for further detailed genetic and antigenic analysis. Len17/1-15 inherited the HA gene from the H5N2-wt and all other genes from Len/17 (7:1 genome composition). The HA gene sequence of Len17/H5 was identical to that of the parent H5N2-wt virus. The antigenic profile of the reassortant virus was similar to that of the H5N2-wt parent strain in the hemagglutination-inhibition (HI) test with a panel of antisera to different avian and human H5 viruses. The reassortant demonstrated high growth ability (9.3+0.3 lg EID50/ml) in embryonated hens' eggs (CE) at optimal (34 degreesC) temperature, comparable with that of the parent Len/17 MS. Also, Len17/H5 demonstrated cold-adapted (ca) and temperature-sensitive (ts) phenotypes similar to those of Len/17 and was attenuated for mice. (C) 2004 Elsevier B.V. All rights reserved.",
keywords = "avian influenza, live attenuated reassortant vaccine",
author = "JA Desheva and LG Rudenko and GI Alexandrova and C.D. Lu and AR Rekstin and JM Katz and NJ Cox and AI Klimov",
year = "2004",
doi = "10.1016/j.ics.2004.02.043",
language = "Английский",
isbn = "0-444-51639-5",
series = "INTERNATIONAL CONGRESS SERIES",
publisher = "Elsevier",
pages = "724--727",
editor = "Y Kawaoka",
booktitle = "OPTIONS FOR THE CONTROL OF INFLUENZA V",
address = "Нидерланды",
note = "null ; Conference date: 07-10-2003 Through 11-10-2003",

}

RIS

TY - GEN

T1 - Reassortment between avian apathogenic and human attenuated cold-adapted viruses as an approach for preparing influenza pandemic vaccines

AU - Desheva, JA

AU - Rudenko, LG

AU - Alexandrova, GI

AU - Lu, C.D.

AU - Rekstin, AR

AU - Katz, JM

AU - Cox, NJ

AU - Klimov, AI

PY - 2004

Y1 - 2004

N2 - To prepare candidate influenza pandemic vaccines, we are developing an approach based on reassortment of antigenically appropriate nonpathogenic avian viruses of different subtypes (H5, H9, H7) with the cold-adapted master strain (MS) A/Leningrad/134/17/57 (Len/17) that is currently used in Russia for preparing licensed live attenuated vaccines for adults and children. In the present study, reassortants between A/Duck/Potsdam/1402-6/86(H5N2) (H5N2-wt) and Len/17 were obtained. One of the clones, A/17/Duck/Potsdam/86-92(H5N2) (Len17/H5), was chosen for further detailed genetic and antigenic analysis. Len17/1-15 inherited the HA gene from the H5N2-wt and all other genes from Len/17 (7:1 genome composition). The HA gene sequence of Len17/H5 was identical to that of the parent H5N2-wt virus. The antigenic profile of the reassortant virus was similar to that of the H5N2-wt parent strain in the hemagglutination-inhibition (HI) test with a panel of antisera to different avian and human H5 viruses. The reassortant demonstrated high growth ability (9.3+0.3 lg EID50/ml) in embryonated hens' eggs (CE) at optimal (34 degreesC) temperature, comparable with that of the parent Len/17 MS. Also, Len17/H5 demonstrated cold-adapted (ca) and temperature-sensitive (ts) phenotypes similar to those of Len/17 and was attenuated for mice. (C) 2004 Elsevier B.V. All rights reserved.

AB - To prepare candidate influenza pandemic vaccines, we are developing an approach based on reassortment of antigenically appropriate nonpathogenic avian viruses of different subtypes (H5, H9, H7) with the cold-adapted master strain (MS) A/Leningrad/134/17/57 (Len/17) that is currently used in Russia for preparing licensed live attenuated vaccines for adults and children. In the present study, reassortants between A/Duck/Potsdam/1402-6/86(H5N2) (H5N2-wt) and Len/17 were obtained. One of the clones, A/17/Duck/Potsdam/86-92(H5N2) (Len17/H5), was chosen for further detailed genetic and antigenic analysis. Len17/1-15 inherited the HA gene from the H5N2-wt and all other genes from Len/17 (7:1 genome composition). The HA gene sequence of Len17/H5 was identical to that of the parent H5N2-wt virus. The antigenic profile of the reassortant virus was similar to that of the H5N2-wt parent strain in the hemagglutination-inhibition (HI) test with a panel of antisera to different avian and human H5 viruses. The reassortant demonstrated high growth ability (9.3+0.3 lg EID50/ml) in embryonated hens' eggs (CE) at optimal (34 degreesC) temperature, comparable with that of the parent Len/17 MS. Also, Len17/H5 demonstrated cold-adapted (ca) and temperature-sensitive (ts) phenotypes similar to those of Len/17 and was attenuated for mice. (C) 2004 Elsevier B.V. All rights reserved.

KW - avian influenza

KW - live attenuated reassortant vaccine

U2 - 10.1016/j.ics.2004.02.043

DO - 10.1016/j.ics.2004.02.043

M3 - статья в сборнике материалов конференции

SN - 0-444-51639-5

T3 - INTERNATIONAL CONGRESS SERIES

SP - 724

EP - 727

BT - OPTIONS FOR THE CONTROL OF INFLUENZA V

A2 - Kawaoka, Y

PB - Elsevier

Y2 - 7 October 2003 through 11 October 2003

ER -

ID: 61121232