Rate of spontaneous polyploidization in haploid yeast Saccharomyces cerevisiae

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Rate of spontaneous polyploidization in haploid yeast Saccharomyces cerevisiae. / Andreychuk, Yulia ; Zhuk, Anna ; Tarakhovskaya, Elena ; Inge-Vechtomov, Sergei ; Stepchenkova, Elena .

In: Biological Communications, Vol. 67, No. 2, 04.2022, p. 88-96.

Research output: Contribution to journal › Article › peer-review

BibTeX

@article{06662746d72048fab4cc8bf4636064dd,

title = "Rate of spontaneous polyploidization in haploid yeast Saccharomyces cerevisiae",

abstract = "Polyploidization is involved in a variety of biological processes. It is one of the principal mechanisms of new species formation since it provides material for gene diversification and subsequent selection. Multiple cases of polyploidization were registered in different branches of the evolutionary tree of eukaryotes. Besides its role in evolution, polyploidization affects gene expression in living cells: pathological genome duplications often happen in cancer cells. The mechanisms and consequences of polyploidization are being studied extensively. However, quantitative determination of the polyploidization rate is challenging due to its low frequency and the absence of selective genetic markers that would phenotypically distinguish between haploids and polyploids. Our work describes a robust and straightforward method for discriminating haploid and polyploid states in the yeast Saccharomyces cerevisiae, a model organism for studying different aspects of polyploidization. The measurement of polyploidization rate showed that in yeast cells this process is mainly caused by autodiploidization rather than mating-type switching followed by hybridization.",

keywords = "Saccharomyces cerevisiae, POLYPLOIDIZATION, haploid, diploid, Whole-genome duplication, polyploidization, whole-genome duplication",

author = "Yulia Andreychuk and Anna Zhuk and Elena Tarakhovskaya and Sergei Inge-Vechtomov and Elena Stepchenkova",

note = "Publisher Copyright: {\textcopyright} 2022 Andreychuk et al.",

year = "2022",

month = apr,

doi = "10.21638/spbu03.2022.202",

language = "English",

volume = "67",

pages = "88--96",

journal = "Biological Communications",

issn = "2542-2154",

publisher = "Издательство Санкт-Петербургского университета",

number = "2",

}

RIS

TY - JOUR

T1 - Rate of spontaneous polyploidization in haploid yeast Saccharomyces cerevisiae

AU - Andreychuk, Yulia

AU - Zhuk, Anna

AU - Tarakhovskaya, Elena

AU - Inge-Vechtomov, Sergei

AU - Stepchenkova, Elena

PY - 2022/4

Y1 - 2022/4

N2 - Polyploidization is involved in a variety of biological processes. It is one of the principal mechanisms of new species formation since it provides material for gene diversification and subsequent selection. Multiple cases of polyploidization were registered in different branches of the evolutionary tree of eukaryotes. Besides its role in evolution, polyploidization affects gene expression in living cells: pathological genome duplications often happen in cancer cells. The mechanisms and consequences of polyploidization are being studied extensively. However, quantitative determination of the polyploidization rate is challenging due to its low frequency and the absence of selective genetic markers that would phenotypically distinguish between haploids and polyploids. Our work describes a robust and straightforward method for discriminating haploid and polyploid states in the yeast Saccharomyces cerevisiae, a model organism for studying different aspects of polyploidization. The measurement of polyploidization rate showed that in yeast cells this process is mainly caused by autodiploidization rather than mating-type switching followed by hybridization.

AB - Polyploidization is involved in a variety of biological processes. It is one of the principal mechanisms of new species formation since it provides material for gene diversification and subsequent selection. Multiple cases of polyploidization were registered in different branches of the evolutionary tree of eukaryotes. Besides its role in evolution, polyploidization affects gene expression in living cells: pathological genome duplications often happen in cancer cells. The mechanisms and consequences of polyploidization are being studied extensively. However, quantitative determination of the polyploidization rate is challenging due to its low frequency and the absence of selective genetic markers that would phenotypically distinguish between haploids and polyploids. Our work describes a robust and straightforward method for discriminating haploid and polyploid states in the yeast Saccharomyces cerevisiae, a model organism for studying different aspects of polyploidization. The measurement of polyploidization rate showed that in yeast cells this process is mainly caused by autodiploidization rather than mating-type switching followed by hybridization.

KW - Saccharomyces cerevisiae

KW - POLYPLOIDIZATION

KW - haploid

KW - diploid

KW - Whole-genome duplication

KW - polyploidization

KW - whole-genome duplication

UR - https://www.mendeley.com/catalogue/966a8635-7560-3134-a777-f1b36c15b52f/

UR - http://www.scopus.com/inward/record.url?scp=85140652693&partnerID=8YFLogxK

U2 - 10.21638/spbu03.2022.202

DO - 10.21638/spbu03.2022.202

M3 - Article

VL - 67

SP - 88

EP - 96

JO - Biological Communications

JF - Biological Communications

SN - 2542-2154

IS - 2

ER -

ID: 98410188