Research output: Contribution to journal › Article › peer-review
Rapid generation of functional dopaminergic neurons from human induced pluripotent stem cells through a single-step procedure using cell lineage transcription factors. / Theka, Ilda; Caiazzo, Massimiliano; Dvoretskova, Elena; Leo, Damiana; Ungaro, Federica; Curreli, Sebastiano; Managò, Francesca; Dell'Anno, Maria Teresa; Pezzoli, Gianni; Gainetdinov, Raul R.; Dityatev, Alexander; Broccoli, Vania.
In: Stem cells translational medicine, Vol. 2, No. 6, 2013, p. 473-479.Research output: Contribution to journal › Article › peer-review
}
TY - JOUR
T1 - Rapid generation of functional dopaminergic neurons from human induced pluripotent stem cells through a single-step procedure using cell lineage transcription factors
AU - Theka, Ilda
AU - Caiazzo, Massimiliano
AU - Dvoretskova, Elena
AU - Leo, Damiana
AU - Ungaro, Federica
AU - Curreli, Sebastiano
AU - Managò, Francesca
AU - Dell'Anno, Maria Teresa
AU - Pezzoli, Gianni
AU - Gainetdinov, Raul R.
AU - Dityatev, Alexander
AU - Broccoli, Vania
PY - 2013
Y1 - 2013
N2 - Current protocols for in vitro differentiation of human induced pluripotent stem cells (hiPSCs) to generate dopamine (DA) neurons are laborious and time-expensive. In order to accelerate the overall process, we have established a fast protocol by expressing the developmental transcription factors ASCL1, NURR1, and LMX1A. With this method, we were able to generate mature and functional dopaminergic neurons in as few as 21 days, skipping all the intermediate steps for inducting and selecting embryoid bodies and rosette-neural precursors. Strikingly, the resulting neuronal conversion process was very proficient, with an overall efficiency that was more than 93% of all the coinfected cells. hiPSC-derived DA neurons expressed all the critical molecular markers of the DA molecular machinery and exhibited sophisticated functional features including spontaneous electrical activity and dopamine release. This one-step protocol holds important implications for in vitro disease modeling and is particularly amenable for exploitation in high-throughput screening protocols.
AB - Current protocols for in vitro differentiation of human induced pluripotent stem cells (hiPSCs) to generate dopamine (DA) neurons are laborious and time-expensive. In order to accelerate the overall process, we have established a fast protocol by expressing the developmental transcription factors ASCL1, NURR1, and LMX1A. With this method, we were able to generate mature and functional dopaminergic neurons in as few as 21 days, skipping all the intermediate steps for inducting and selecting embryoid bodies and rosette-neural precursors. Strikingly, the resulting neuronal conversion process was very proficient, with an overall efficiency that was more than 93% of all the coinfected cells. hiPSC-derived DA neurons expressed all the critical molecular markers of the DA molecular machinery and exhibited sophisticated functional features including spontaneous electrical activity and dopamine release. This one-step protocol holds important implications for in vitro disease modeling and is particularly amenable for exploitation in high-throughput screening protocols.
KW - Direct cell conversion
KW - Dopamine
KW - Neuron
KW - Pluripotent stem cells
KW - Reprogramming
UR - http://www.scopus.com/inward/record.url?scp=84878759743&partnerID=8YFLogxK
U2 - 10.5966/sctm.2012-0133
DO - 10.5966/sctm.2012-0133
M3 - Article
AN - SCOPUS:84878759743
VL - 2
SP - 473
EP - 479
JO - Stem cells translational medicine
JF - Stem cells translational medicine
SN - 2157-6564
IS - 6
ER -
ID: 99380427