Links

DOI

  • J. Dong
  • Q. Wang
  • T. Gu
  • G. Liu
  • Y.V. Petrov
  • V.E. Baulin
  • A.Yu Tsivadze
  • D. Jia
  • Y. Zhou
  • H. Yuan
  • B. Li
Suppression of vascular cell senescence is of great significance in preventing cardiovascular diseases such as hypertension and atherosclerosis. The oxidative stress damage caused by reactive oxygen species (ROS) can lead to cellular senescence. Rapamycin (Rapa) is well known to suppress cell senescence via mammalian target of rapamycin (mTOR) pathway. However, poor water solubility and lack of ROS scavenging ability limit the further development of Rapa. To improve the solubility of Rapa and endow with ROS scavenging ability, Rapa functionalized carbon dots (Rapa-CDs) are target-oriented synthesized via free radical polymerization combination with hydrothermal carbonization. Rapa-CDs improve the solubility of Rapa and show ROS scavenging abilities. The solubility of Rapa-CDs with 9.41 g is improved 3.6 × 104 times higher than that of Rapa (2.6 × 10-4 g). The half maximal inhibitory concentration (IC50) of Rapa-CDs toward hydroxyl radical (•OH) and 2,2-Diphenyl-1-picrylhydrazyl free radical (DPPH•) are 0.18 and 0.17 mg/mL, respectively. Rapa-CDs show anti-oxidative stress effect in HEVECs (Human Umbilical Vein Endothelial Cells) via reducing ROS levels by 87 %. Rapa-CDs alleviate HUVECs senescence by suppressing mTOR overactivation, attenuate the expression of P53, P21 and P16. The study demonstrates the target-oriented synthesis of drugs functionalized CDs with anti-senescence via dual-pathway of anti-oxidative stress and mTOR. © 2024 Elsevier Inc.
Original languageEnglish
Pages (from-to)534-544
Number of pages11
JournalJournal of Colloid and Interface Science
Volume660
DOIs
StatePublished - 1 Apr 2024

    Research areas

  • Carbon dots, Cell senescence, Chitosan, Rapamycin, ROS, Target-oriented synthesis, Antibiotics, Carbon, Carbonization, Controlled drug delivery, Cytology, Diseases, Endothelial cells, Mammals, Oxidative stress, Solubility, Cell senescences, Functionalized, Mammalian target, Reactive oxygen species, Scavenging ability, Target of rapamycin, Target oriented, Free radicals, carbon, chitosan, hydroxyl radical, mammalian target of rapamycin, protein p16, protein p21, protein p53, reactive oxygen metabolite, sirolimus, Article, carbonization, cell aging, human, human cell, IC50, mTOR signaling, oxidative stress, polymerization, signal transduction, synthesis, umbilical vein endothelial cell, vascularization, water solubility, article, atherosclerosis, controlled study, nonhuman, pharmacology

ID: 117803161